MicroRNA-148a inhibits migration and invasion of ovarian cancer cells via targeting sphingosine-1-phosphate receptor 1 Retraction in /10.3892/mmr.2021.12298

Wen,Zhengfang; Zhao,Shuzhen; Liu,Shanshan; Liu,Ying; Li,Xiaorui; Li,Shaoru

doi:10.3892/mmr.2015.3827

September-2015 Volume 12 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

September-2015 Volume 12 Issue 3

Full Size Image

Article

MicroRNA-148a inhibits migration and invasion of ovarian cancer cells via targeting sphingosine-1-phosphate receptor 1

Retraction in: /10.3892/mmr.2021.12298

Authors:
- Zhengfang Wen
- Shuzhen Zhao
- Shanshan Liu
- Ying Liu
- Xiaorui Li
- Shaoru Li
View Affiliations / Copyright

Affiliations: Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Oncology, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China
Pages: 3775-3780
|
Published online on: May 25, 2015

https://doi.org/10.3892/mmr.2015.3827
Expand metrics +

Abstract

MicroRNAs, a group of small non-coding RNA molecules that are involved in gene silencing, function as fine‑tuning regulators during cancer progression. MicroRNA (miR)-148a has previously been demonstrated to be associated with ovarian cancer. However, whether miR‑148a influences the migration and invasion of ovarian cancer cells has remained elusive. In the present study, reverse transcription‑quantitative polymerase chain reaction and western blotting were conducted to examine mRNA and protein expression levels, respectively. Luciferase reporter assay was used to determine target relationship, and a Transwell assay was used to study cell migration and invasion. The results of the present study indicated that miR‑148a expression was markedly downregulated in ovarian cancer tissues compared with that of their matched normal adjacent tissues. In addition, miR‑148a expression levels were reduced in three ovarian cancer cell lines, SKOV3, OVCAR and A2780, when compared with those of HUM‑CELL‑0088 normal ovarian epithelial cells. Furthermore, sphingosine‑1‑phosphate receptor 1 (S1PR1), which is upregulated in ovarian cancer tissues and cell lines, was identified as a novel target of miR‑148a in SKOV3 ovarian cancer cells. The protein expression of S1PR1 was negatively regulated by miR‑148a in SKOV3 cells. Furthermore, overexpression of miR‑148a or inhibition of S1PR1 suppressed SKOV3 cell migration and invasion, while restoration of S1PR1 expression reversed the suppressive effect of miR‑148a upregulation on SKOV3 cell migration and invasion. In conclusion, it was hypothesized that miR‑148a may potentially be used as a molecular agent for the prevention and treatment of invasion and metastasis in ovarian cancer, while S1PR1 may present a promising target for clinical applications.

View Figures

View References

1	Miles GD, Seiler M, Rodriguez L, Rajagopal G and Bhanot G: Identifying microRNA/mRNA dysregulations in ovarian cancer. BMC Res Notes. 5:1642012. View Article : Google Scholar : PubMed/NCBI
2	Ozols RF: Treatment goals in ovarian cancer. Int J Gynecol Cancer. 15(Suppl 1): S3–S11. 2005. View Article : Google Scholar
3	Kleppe M, Amkreutz LC, Van Gorp T, Slangen BF, Kruse AJ and Kruitwagen RF: Lymph-node metastasis in stage I and II sex cord stromal and malignant germ cell tumours of the ovary: A systematic review. Gynecol Oncol. 133:124–127. 2014. View Article : Google Scholar : PubMed/NCBI
4	Titone R, Morani F, Follo C, Vidoni C, Mezzanzanica D and Isidoro C: Epigenetic control of autophagy by microRNAs in ovarian cancer. Biomed Res Int. 2014:3435422014. View Article : Google Scholar : PubMed/NCBI
5	Imbar T and Eisenberg I: Regulatory role of microRNAs in ovarian function. Fertil Steril. 101:1524–1530. 2014. View Article : Google Scholar : PubMed/NCBI
6	Llauradó M, Majem B, Altadill T, et al: MicroRNAs as prognostic markers in ovarian cancer. Mol Cell Endocrinol. 390:73–84. 2014. View Article : Google Scholar : PubMed/NCBI
7	Xia J, Guo X, Yan J and Deng K: The role of miR-148a in gastric cancer. J Cancer Res Clin Oncol. 140:1451–1456. 2014. View Article : Google Scholar : PubMed/NCBI
8	Takahashi M, Cuatrecasas M, Balaguer F, et al: The clinical significance of MiR-148a as a predictive biomarker in patients with advanced colorectal cancer. PLoS One. 7:e466842012. View Article : Google Scholar : PubMed/NCBI
9	Li J, Song Y, Wang Y, Luo J and Yu W: MicroRNA-148a suppresses epithelial-to-mesenchymal transition by targeting ROCK1 in non-small cell lung cancer cells. Mol Cell Biochem. 380:277–282. 2013. View Article : Google Scholar : PubMed/NCBI
10	Zhou X, Zhao F, Wang ZN, et al: Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation. Oncol Rep. 27:447–454. 2012.
11	Tabasinezhad M, Samadi N, Ghanbari P, et al: Sphingosin 1-phosphate contributes in tumor progression. J Cancer Res Ther. 9:556–563. 2013. View Article : Google Scholar
12	Selvam SP and Ogretmen B: Sphingosine kinase/sphingosine 1-phosphate signaling in cancer therapeutics and drug resistance. Handb Exp Pharmacol. 216:3–27. 2013. View Article : Google Scholar : PubMed/NCBI
13	Lin H, Dai T, Xiong H, et al: Unregulated miR-96 induces cell proliferation in human breast cancer by downregulating transcriptional factor FOXO3a. PloS one. 5:e157972010. View Article : Google Scholar
14	Zheng B, Liang L, Wang C, et al: MicroRNA-148a suppresses tumor cell invasion and metastasis by downregulating ROCK1 in gastric cancer. Clin Cancer Res. 17:7574–7583. 2011. View Article : Google Scholar : PubMed/NCBI
15	Fujita Y, Kojima K, Ohhashi R, et al: MiR-148a attenuates paclitaxel resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression. J Biol Chem. 285:19076–19084. 2010. View Article : Google Scholar : PubMed/NCBI
16	Hong G, Baudhuin LM and Xu Y: Sphingosine-1-phosphate modulates growth and adhesion of ovarian cancer cells. FEBS Lett. 460:513–518. 1999. View Article : Google Scholar : PubMed/NCBI
17	Wang D, Zhao Z, Caperell-Grant A, et al: S1P differentially regulates migration of human ovarian cancer and human ovarian surface epithelial cells. Mol Cancer Ther. 7:1993–2002. 2008. View Article : Google Scholar : PubMed/NCBI
18	Dai L, Xia P and Di W: Sphingosine 1-phosphate: A potential molecular target for ovarian cancer therapy? Cancer Invest. 32:71–80. 2014. View Article : Google Scholar : PubMed/NCBI
19	Smicun Y, Reierstad S, Wang FQ, Lee C and Fishman DA: S1P regulation of ovarian carcinoma invasiveness. Gynecol Oncol. 103:952–959. 2006. View Article : Google Scholar : PubMed/NCBI
20	Park KS, Kim MK, Lee HY, et al: S1P stimulates chemotactic migration and invasion in OVCAR3 ovarian cancer cells. Biochem Biophys Res Commun. 356:239–244. 2007. View Article : Google Scholar : PubMed/NCBI
21	Bradaric MJ, Barua A, Penumatsa K, et al: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 4:42011. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA-148a inhibits migration and invasion of ovarian cancer cells via targeting sphingosine-1-phosphate receptor 1

Retraction in: /10.3892/mmr.2021.12298

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles