Open Access

Overexpression of caudal‑related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice

  • Authors:
    • Jian‑Bao Zheng
    • Li‑Na Qiao
    • Xue‑Jun Sun
    • Jie Qi
    • Hai‑Liang Ren
    • Guang‑Bing Wei
    • Pei‑Hua Zhou
    • Jian‑Feng Yao
    • Li Zhang
    • Peng‑Bo Jia
  • View Affiliations

  • Published online on: May 25, 2015     https://doi.org/10.3892/mmr.2015.3838
  • Pages: 3409-3415
  • Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Caudal‑related homeobox transcription factor 2 (CDX2) is a transcription factor, which is specifically expressed in the adult intestine. It is essential for the development and homeostasis of the intestinal epithelium and its functions as a tumor suppressor have been demonstrated in the adult colon. The present study aimed to examine the inhibitory effects of the overexpression of CDX2 on subcutaneously‑transplanted tumors, derived from LoVo colon cancer cells, in nude mice, and to provide experimental evidence for the biotherapy of colon cancer. A pEGFP‑C1‑CDX2 eukaryotic expression vector was transfected into the LoVo cells via lipofection, and LoVo cells stably‑expressing CDX2 (pEGFP‑C1‑CDX2 cells) were obtained using G418 selection. A nude mouse subcutaneously‑transplanted tumor model was established by inoculating the nude mice with the pEGFP‑C1‑CDX2 cells, and the effects of overexpression of CDX2 on transplanted tumor growth in the LoVo cells were observed. Western blotting results demonstrated that the protein expression of CDX2 in the LoVo cells was higher in the pEGFP‑C1‑CDX2 cell group, compared with that in the pEGFP‑C1 cell group and the untreated cell group. At 20 days post‑inoculation with either pEGFP‑C1‑CDX2 or pEGFP‑C1, the transplanted tumor masses were significantly lower in the pEGFP‑C1‑CDX2 group, compared with those in the pEGFP‑C1 and untreated groups. Immunohistochemistry revealed that the expression levels of CDX2 and matrix metalloproteinase‑2 (MMP‑2) were detected in each group, and the protein expression of CDX2 was increased in the tumor tissues from the nude mice in the pEGFP‑C1‑CDX2 group. However the expression of MMP‑2 was downregulated in the tumor tissues of the nude mice in the pEGFP‑C1‑CDX2 group. Taken together, these data suggested that pEGFP‑C1‑CDX2 cells exhibited suppressed tumor growth in vivo. Overexpression of CDX2 was observed in transplanted tumors in the pEGFP‑C1‑CDX2 group, and the gene expression of MMP‑2 was reduced. These results indicate that CDX2 inhibited the growth of colorectal tumor cells, possibly by downregulating the gene expression.
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September-2015
Volume 12 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zheng JB, Qiao LN, Sun XJ, Qi J, Ren HL, Wei GB, Zhou PH, Yao JF, Zhang L, Jia PB, Jia PB, et al: Overexpression of caudal‑related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice. Mol Med Rep 12: 3409-3415, 2015
APA
Zheng, J., Qiao, L., Sun, X., Qi, J., Ren, H., Wei, G. ... Jia, P. (2015). Overexpression of caudal‑related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice. Molecular Medicine Reports, 12, 3409-3415. https://doi.org/10.3892/mmr.2015.3838
MLA
Zheng, J., Qiao, L., Sun, X., Qi, J., Ren, H., Wei, G., Zhou, P., Yao, J., Zhang, L., Jia, P."Overexpression of caudal‑related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice". Molecular Medicine Reports 12.3 (2015): 3409-3415.
Chicago
Zheng, J., Qiao, L., Sun, X., Qi, J., Ren, H., Wei, G., Zhou, P., Yao, J., Zhang, L., Jia, P."Overexpression of caudal‑related homeobox transcription factor 2 inhibits the growth of transplanted colorectal tumors in nude mice". Molecular Medicine Reports 12, no. 3 (2015): 3409-3415. https://doi.org/10.3892/mmr.2015.3838