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Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3

  • Authors:
    • Jingwei Yu
    • Cuifen Bao
    • Yanru Dong
    • Xia Liu
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Key Laboratory of Molecular Cell Biology and New Drug Development, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3339-3344
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/mmr.2015.3850
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Abstract

The present study aimed to investigate the activation of Atg1/pULK, and LC3 in the cerebral cortex following focal cerebral ischemia reperfusion (CIR) injury, thereby examining its effect on autophagy in brain cells. Rat CIR models were established using the technique of middle cerebral artery occlusion. The neurological function score, TTC staining and the water content of brain tissue were used to evaluate the CIR model. Levels of autophagy in the brain cells were examined at different time‑points following CIR damage using electron microscopy. Immunohistochemistry and western blot analysis were also used for the qualitative and quantitative detection of levels of Atg1/pULK and LC3 in the cerebral cortex. Autophagy was observed in the early stage of CIR, and the expression of Atg1/pULK and LC3 were observed 1 h following CIR in the rats and reached peak expression levels after12 h, which following which the they gradually decreased. These results suggested Atg1/pULK and LC3 are key in the regulation of autophagy following CIR in the rat brain.
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Copy and paste a formatted citation
Spandidos Publications style
Yu J, Bao C, Dong Y and Liu X: Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3. Mol Med Rep 12: 3339-3344, 2015.
APA
Yu, J., Bao, C., Dong, Y., & Liu, X. (2015). Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3. Molecular Medicine Reports, 12, 3339-3344. https://doi.org/10.3892/mmr.2015.3850
MLA
Yu, J., Bao, C., Dong, Y., Liu, X."Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3". Molecular Medicine Reports 12.3 (2015): 3339-3344.
Chicago
Yu, J., Bao, C., Dong, Y., Liu, X."Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3". Molecular Medicine Reports 12, no. 3 (2015): 3339-3344. https://doi.org/10.3892/mmr.2015.3850
Copy and paste a formatted citation
x
Spandidos Publications style
Yu J, Bao C, Dong Y and Liu X: Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3. Mol Med Rep 12: 3339-3344, 2015.
APA
Yu, J., Bao, C., Dong, Y., & Liu, X. (2015). Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3. Molecular Medicine Reports, 12, 3339-3344. https://doi.org/10.3892/mmr.2015.3850
MLA
Yu, J., Bao, C., Dong, Y., Liu, X."Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3". Molecular Medicine Reports 12.3 (2015): 3339-3344.
Chicago
Yu, J., Bao, C., Dong, Y., Liu, X."Activation of autophagy in rat brain cells following focal cerebral ischemia reperfusion through enhanced expression of Atg1/pULK and LC3". Molecular Medicine Reports 12, no. 3 (2015): 3339-3344. https://doi.org/10.3892/mmr.2015.3850
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