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Article

Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes

  • Authors:
    • Libin Yang
    • Zhixia Bai
    • Danni Lv
    • Haibo Liu
    • Xiaohui Li
    • Xuexin Chen
  • View Affiliations / Copyright

    Affiliations: Clinical Medicine School, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Anesthesiology, Tumor Hospital of the General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
  • Pages: 3739-3747
    |
    Published online on: May 26, 2015
       https://doi.org/10.3892/mmr.2015.3852
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Abstract

The aim of the present study was to investigate the mechanism underlying the rescue effect of lipid emulsion on bupivacaine (BPV)‑induced cardiomyocyte toxicity. The inhibitory effects of BPV on H9c2 myoblast cell proliferation were investigated using an MTT assay. The H9c2 myoblast cells were treated with either 1 mM BPV or 1% lipid emulsion (LE) alone, or co‑treated with both of the drugs. Cell apoptosis was detected using both Annexin V/propidium iodide staining and a terminal deoxynucleotidyl transferase dUTP assay. The protein expression levels of apoptosis-associated proteins were quantified using western blot analysis, and the mRNA expression levels were quantified by reverse transcription‑quantitative polymerase chain reaction. The expression levels of reactive oxidative species, malondialdehyde, superoxide dismutase, and catalase were quantified using the optical density values obtained from a spectrophotometer. In addition, the mechanism underlying the mitochondrial function of the H9c2 myoblast cells was investigated using both JC‑1 staining, and cyclosporin A and atractyloside treatment. The results indicated that the H9c2 myoblast cells treated with BPV exhibited significantly higher levels of apoptosis. Furthermore, BPV treatment increased the levels of oxidative stress, and caused mitochondrial dysfunction within the H9c2 myoblast cells. LE treatment reversed the effects of BPV treatment in the H9c2 myoblast cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Bai Z, Lv D, Liu H, Li X and Chen X: Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes. Mol Med Rep 12: 3739-3747, 2015.
APA
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., & Chen, X. (2015). Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes. Molecular Medicine Reports, 12, 3739-3747. https://doi.org/10.3892/mmr.2015.3852
MLA
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., Chen, X."Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes". Molecular Medicine Reports 12.3 (2015): 3739-3747.
Chicago
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., Chen, X."Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes". Molecular Medicine Reports 12, no. 3 (2015): 3739-3747. https://doi.org/10.3892/mmr.2015.3852
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Bai Z, Lv D, Liu H, Li X and Chen X: Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes. Mol Med Rep 12: 3739-3747, 2015.
APA
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., & Chen, X. (2015). Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes. Molecular Medicine Reports, 12, 3739-3747. https://doi.org/10.3892/mmr.2015.3852
MLA
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., Chen, X."Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes". Molecular Medicine Reports 12.3 (2015): 3739-3747.
Chicago
Yang, L., Bai, Z., Lv, D., Liu, H., Li, X., Chen, X."Rescue effect of lipid emulsion on bupivacaine‑induced cardiac toxicity in cardiomyocytes". Molecular Medicine Reports 12, no. 3 (2015): 3739-3747. https://doi.org/10.3892/mmr.2015.3852
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