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Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues

  • Authors:
    • Pengfei Hu
    • Yabin Pu
    • Xiayun Li
    • Zhiqiang Zhu
    • Yuhua Zhao
    • Weijun Guan
    • Yuehui Ma
  • View Affiliations / Copyright

    Affiliations: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China, Harbin Institute of Physical Education, Harbin, Heilongjiang 150008, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3331-3338
    |
    Published online on: May 27, 2015
       https://doi.org/10.3892/mmr.2015.3854
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Abstract

Lung‑derived mesenchymal stem cells (LMSCs) are considered to be important in lung tissue repair and regenerative processes. However, the biological characteristics and differentiation potential of LMSCs remain to be elucidated. In the present study, fetal lung‑derived mesenchymal stem cells (FLMSCs) were isolated from fetal bovine lung tissues by collagenase digestion. The in vitro culture conditions were optimized and stabilized and the self‑renewal ability and differentiation potential were evaluated. The results demonstrated that the FLMSCs were morphologically consistent with fibroblasts, were able to be cultured and passaged for at least 33 passages and the cell morphology and proliferative ability were stable during the first 10 passages. In addition, FLMSCs were found to express CD29, CD44, CD73 and CD166, however, they did not express hematopoietic cell specific markers, including CD34, CD45 and BOLA‑DRα. The growth kinetics of FLMSCs consisted of a lag phase, a logarithmic phase and a plateau phase, and as the passages increased, the proliferative ability of cells gradually decreased. The majority of FLMSCs were in G0/G1 phase. Following osteogenic induction, FLMSCs were positive for the expression of osteopontin and collagen type I α2. Following neurogenic differentiation, the cells were morphologically consistent with neuronal cells and positive for microtubule‑associated protein 2 and nestin expression. It was concluded that the isolated FLMSCs exhibited typical characteristics of mesenchymal stem cells and that the culture conditions were suitable for their proliferation and the maintenance of stemness. The present study illustrated the potential application of lung tissue as an adult stem cell source for regenerative therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Hu P, Pu Y, Li X, Zhu Z, Zhao Y, Guan W and Ma Y: Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues. Mol Med Rep 12: 3331-3338, 2015.
APA
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., & Ma, Y. (2015). Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues. Molecular Medicine Reports, 12, 3331-3338. https://doi.org/10.3892/mmr.2015.3854
MLA
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., Ma, Y."Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues". Molecular Medicine Reports 12.3 (2015): 3331-3338.
Chicago
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., Ma, Y."Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues". Molecular Medicine Reports 12, no. 3 (2015): 3331-3338. https://doi.org/10.3892/mmr.2015.3854
Copy and paste a formatted citation
x
Spandidos Publications style
Hu P, Pu Y, Li X, Zhu Z, Zhao Y, Guan W and Ma Y: Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues. Mol Med Rep 12: 3331-3338, 2015.
APA
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., & Ma, Y. (2015). Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues. Molecular Medicine Reports, 12, 3331-3338. https://doi.org/10.3892/mmr.2015.3854
MLA
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., Ma, Y."Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues". Molecular Medicine Reports 12.3 (2015): 3331-3338.
Chicago
Hu, P., Pu, Y., Li, X., Zhu, Z., Zhao, Y., Guan, W., Ma, Y."Isolation, in vitro culture and identification of a new type of mesenchymal stem cell derived from fetal bovine lung tissues". Molecular Medicine Reports 12, no. 3 (2015): 3331-3338. https://doi.org/10.3892/mmr.2015.3854
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