Obesity‑associated microRNA‑26b regulates the proliferation of human preadipocytes via arrest of the G1/S transition

Xu,Guangfeng; Ji,Chenbo; Song,Guixian; Shi,Chunmei; Shen,Yahui; Chen,Ling; Yang,Lei; Zhao,Yaping; Guo,Xirong

doi:10.3892/mmr.2015.3858

September-2015 Volume 12 Issue 3

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September-2015 Volume 12 Issue 3

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Article

Obesity‑associated microRNA‑26b regulates the proliferation of human preadipocytes via arrest of the G1/S transition

Authors:
- Guangfeng Xu
- Chenbo Ji
- Guixian Song
- Chunmei Shi
- Yahui Shen
- Ling Chen
- Lei Yang
- Yaping Zhao
- Xirong Guo
View Affiliations / Copyright

Affiliations: Department of Laboratory Medicine, The 82nd Hospital of The People's Liberation Army, Huaian, Jiangsu 223001, P.R. China, Department of Child Health Care, Affiliated Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Institute of Pediatrics, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Pages: 3648-3654
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Published online on: May 27, 2015

https://doi.org/10.3892/mmr.2015.3858
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Abstract

MicroRNAs (miRNAs) are short, 20‑24 nucleotide non‑coding RNAs, which are involved in multiple biological processes, including obesity. Our previous investigation revealed that miRNA (miR)‑26b is differentially expressed in preadipocytes and mature adipocytes in humans. However, its role in the proliferation of human preadipocytes remains to be fully elucidated. In the present study, intracellular lipid accumulation was assessed using oil red O staining and the trigycerlide (TG) content was quantified using a TG assay kit, adipogenesis associated genes and cyclin D2 were analyzed using western blotting, and the effects of miR‑26b on the proliferation of preadipocytes was investigated using Cell Counting Kit‑8 assays and cell cycle analysis. Human preadipocytes overexpressing miR‑26b exhibited increased TG content in the adipocytes. During differentiation, the protein expression levels of adipogenesis‑associated marker genes, including peroxisome proliferator‑activated receptor γ, CCAAT/enhancer‑binding protein α, fatty acid‑binding protein and hormone‑sensitive lipase were upregulated in cells overexpressing miR‑26b, compared with the negative control cells. In addition, growth of human preadipocytes overexpressing miR‑26b occurred a slower rate and more remained in the G1 phase, compared with the negative control cells. In addition, miR‑26b downregulated the protein expression of cyclin D2. These results demonstrated that miR‑26b promoted differentiation and, at least party by targeting cyclin D2, attenuated cell proliferation via arresting the G1/S transition.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Obesity‑associated microRNA‑26b regulates the proliferation of human preadipocytes via arrest of the G1/S transition

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