Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells

Retraction in: /10.3892/mmr.2023.12965
  • Authors:
    • Yuncheng Qiao
    • Dongming Guo
    • Lei Meng
    • Qingnan Liu
    • Xiaohui Liu
    • Chaoke Tang
    • Guanghui Yi
    • Zuo Wang
    • Weidong Yin
    • Guoping Tian
    • Zhonghua Yuan
  • View Affiliations / Copyright

    Affiliations: Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Basic Nursing, Yiyang Medical College, Yiyang, Hunan 413000, P.R. China, Cyrus Tang Hematology Center (Research Partnership), Jiangsu Institute of Hematology, The First Affiliated Hospital, Soochow University, Suzhou, Jiangsu 215400, P.R. China, Department of Cardiovascular Medicine, The Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 3599-3606
    |
    Published online on: May 27, 2015
       https://doi.org/10.3892/mmr.2015.3864
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Oxidized low‑density lipoprotein (ox‑LDL) can increase the expression of adipophilin and the accumulation of intracellular lipid droplets. However, the detailed mechanisms remain to be fully elucidated. The present study aimed to investigate the mechanism underlying the effect of ox‑LDL on the expression of adipophilin and the accumulation of intracellular cholesterol esters. The results revealed that ox‑LDL increased the activation of protein kinase C α (PKCα), expression of adipophilin and acyl‑coenzymeA: cholesterol acyltransferse 1 (ACAT1) and increased accumulation of intracellular cholesterol esters. In addition, PKCα siRNA abrogated ox‑LDL‑induced adipophilin, expression of ATAC1 and accumulation of cholesterol esters. Furthermore, ox‑LDL increased the accumulation of intracellular cholesterol esters and expression of ACAT1, and this effect were reversed by transfection with adipophilin siRNA. Taken together, these results demonstrated that ox‑LDL induces the accumulation of cholesterol esters, which is mediated by the PKCα‑adipophilin‑ACAT1 pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Paim LR, Schreiber R, Matos-Souza JR, Silva AA, Campos LF, et al: Oxidized low-density lipoprotein, matrix-metalloproteinase-8 and carotid atherosclerosis in spinal cord injured subjects. Atherosclerosis. 231:341–345. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Pirillo A, Norata GD and Catapano AL: LOX-1, OxLDL and atherosclerosis. Mediators Inflamm. 2013:1527862013. View Article : Google Scholar

3 

Yang H, Mohamed AS and Zhou SH: Oxidized low density lipoprotein, stem cells and atherosclerosis. Lipids Health Dis. 11:2–9. 2012. View Article : Google Scholar

4 

Straub BK, Gyoengyoesi B, Koenig M, Hashani M, Pawella LM, et al: Adipophilin/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation. Histopathology. 62:617–631. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Buers I, Hofnagel O, Ruebel A, Severs NJ and Robenek H: Lipid droplet associated proteins: an emerging role in atherogenesis. Histol Histopathol. 26:631–642. 2011.PubMed/NCBI

6 

Song Y, Li H, Zhang LY, Ye Q and Li Q: Expression profile of ADRP in macrophages and foam cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 25:309–311. 2009.In Chinese. PubMed/NCBI

7 

Nuotio K, Isoviita PM, Saksi J, Ijäs P, Pitkäniemi J, Sonninen R, et al: Adipophilin expression is increased in symptomatic carotid atherosclerosis: correlation with red blood cells and cholesterol crystals. Stroke. 38:1791–1798. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Via DP, Pons L, Dennison DK, et al: Induction of acetyl' LDL receptor activity by phorbol ester in human monocyte cell line THP-1. J Lipid Res. 30:1515–1524. 1989.PubMed/NCBI

9 

Slater SJ, Seiz JL, Cook AC, Stagliano BA and Buzas CJ: Inhibition of protein kinase C by resveratrol. Biochim Biophys Acta. 1637:59–69. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Freeman NE, Rusinol AE, Linton M, Hachey DL, Fazio S, Sinensky MS and Thewke D: Acyl-coenzyme A: Cholesterol acyltransferase promotes oxidized LDL/oxysterol-induced apoptosis in macrophages. J Lipid Res. 46:1933–1943. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Liang Y, Yang X, Ma L, Cai X, Wang L, Yang C, et al: Homocysteine-mediated cholesterol efflux via ABCA1 and ACAT1 DNA methylation in THP-1 monocyte-derived foam cells. Acta Biochim Biophys Sin (Shanghai). 45:220–228. 2013. View Article : Google Scholar

12 

Robenek H, Robenek MJ and Troyer D: PAT family proteins pervade lipid droplet cores. J Lipid Res. 46:1331–1338. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Gao J, Ye H and Serrero G: Stimulation of adipose differentiation related protein (ADRP) expression in adipocyte precursors by long-chain fatty acids. J Cell Physiol. 182:297–302. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Liu Q, Dai Z, Liu Z, Liu X, Tang C, Wang Z, et al: Oxidized low-density lipoprotein activates adipophilin through ERK1/2 signal pathway in RAW264.7 cells. Acta Biochim Biophys Sin (Shanghai). 42:635–645. 2010. View Article : Google Scholar

15 

Yuan ZH, Liu XH, Wang ZQ, Jia W, Niu XL, Huang AF, et al: Adipophilin accumulates cellular cholesteryl ester by PKC and ACAT1 in THP-1 macrophages. Chinese J Pathophysiol. 24:833–842. 2008.In Chinese.

16 

Qiao YC, Huang AF, Tian GP, Liu XH and Yuan ZH: Adipophilin facilitate Acyl-coenzyme A: cholesterol acyltransferase 1 expression in RAW264.7 cells. Chin J Arterioscler. 17:643–647. 2009.In Chinese.

17 

Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, et al: Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol. 25:1711–1717. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Thomas JP, Geiger PG, Maiorino M, Ursini F and Girotti AW: Enzymatic reduction of phospholipid and cholesterol hydroper-oxides in artificial bilayers and lipoproteins. Biochim Biophys Acta. 1045:252–260. 1990. View Article : Google Scholar : PubMed/NCBI

19 

Chang TY, Li BL, Chang CC and Urano Y: Acyl-coenzyme A: cholesterol acyltransferases. Am J Physiol Endocrinol Metab. 297:E1–E9. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Larigauderie G, Furman C, Jaye M, Lasselin C, Copin C, Fruchart JC, et al: Adipophilin enhances lipid accumulation and prevents lipid efflux from THP-1 macrophages: Potential role in atherogenesis. Arterioscler Thromb Vasc Biol. 24:504–510. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Imamura M, Inoguchi J, Ikuyama S, Taniguchi S, Kobayashi K, Nakashima N and Nawata H: ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. Am J Physiol Endocrinol Metab. 283:E775–E783. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Yuan ZH, Yang YZ, Yin WD, et al: Induced expression of adipo-philin with high cholesterol diet in rabbit atherosclerotic lesions and reduced lipid accumulation with adipophilin antisense in mouse macrophages. Prog Biochem Biophys. 30:549–554. 2003.

23 

Kitayama K, Tanimoto T, Koga T, Terasaka N, Fujioka T, Inaba T, et al: Importance of acyl-coenzyme A:cholesterol acyl-transferase 1/2 dual inhibition for anti-atherosclerotic potency of pactimibe. Eur J Pharmacol. 540:121–130. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Rong JX, Blachford C, Feig JE, Bander I, Mayne J, Kusunoki J, et al: ACAT inhibition reduces the progression of preexisting, advanced atherosclerotic mouse lesions without plaque or systemic toxicity. Arterioscler Thromb Vasc Biol. 33:4–12. 2013. View Article : Google Scholar :

25 

Yang L, Yang JB, Chen J, Yu GY, Zhou P, Lei L, et al: Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res. 14:315–323. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Daugherty A, Rateri DL and Lu H: As macrophages indulge, atherosclerotic lesions bulge. Circ Res. 102:1445–1447. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Kruth HS, Huang W, Ishii I and Zhang WY: Macrophage foam cell formation with native low density lipoprotein. J Biol Chem. 277:34573–34580. 2002. View Article : Google Scholar : PubMed/NCBI

28 

Karunakaran D, Kockx M, Owen DM, Burnett JR, Jessup W and Kritharides L: Protein kinase C controls vesicular transport and secretion of apolipoprotein E from primary human macrophages. J Biol Chem. 288:5186–5197. 2013. View Article : Google Scholar : PubMed/NCBI

29 

von Knethen A, Soller M, Tzieply N, Weigert A and Johann AM: PPARgamma1 attenuates cytosol to membrane translocation of PKCalpha to desensitize monocytes/macrophages. J Cell Biol. 176:681–694. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Kang J, Cheng B and Jiang L: PPARγ signal transduction pathway in the foam cell formation induced by visfatin. Sheng Li Xue Bao. 62:427–432. 2010.In Chinese. PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Qiao Y, Guo D, Meng L, Liu Q, Liu X, Tang C, Yi G, Wang Z, Yin W, Tian G, Tian G, et al: Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965. Mol Med Rep 12: 3599-3606, 2015.
APA
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C. ... Yuan, Z. (2015). Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965. Molecular Medicine Reports, 12, 3599-3606. https://doi.org/10.3892/mmr.2015.3864
MLA
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C., Yi, G., Wang, Z., Yin, W., Tian, G., Yuan, Z."Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965". Molecular Medicine Reports 12.3 (2015): 3599-3606.
Chicago
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C., Yi, G., Wang, Z., Yin, W., Tian, G., Yuan, Z."Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965". Molecular Medicine Reports 12, no. 3 (2015): 3599-3606. https://doi.org/10.3892/mmr.2015.3864
Copy and paste a formatted citation
x
Spandidos Publications style
Qiao Y, Guo D, Meng L, Liu Q, Liu X, Tang C, Yi G, Wang Z, Yin W, Tian G, Tian G, et al: Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965. Mol Med Rep 12: 3599-3606, 2015.
APA
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C. ... Yuan, Z. (2015). Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965. Molecular Medicine Reports, 12, 3599-3606. https://doi.org/10.3892/mmr.2015.3864
MLA
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C., Yi, G., Wang, Z., Yin, W., Tian, G., Yuan, Z."Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965". Molecular Medicine Reports 12.3 (2015): 3599-3606.
Chicago
Qiao, Y., Guo, D., Meng, L., Liu, Q., Liu, X., Tang, C., Yi, G., Wang, Z., Yin, W., Tian, G., Yuan, Z."Oxidized‑low density lipoprotein accumulates cholesterol esters via the PKCα‑adipophilin‑ACAT1 pathway in RAW264.7 cells Retraction in /10.3892/mmr.2023.12965". Molecular Medicine Reports 12, no. 3 (2015): 3599-3606. https://doi.org/10.3892/mmr.2015.3864
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team