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Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis

  • Authors:
    • Hui‑Ning Fan
    • Ni‑Wei Chen
    • Wei‑Lin Shen
    • Xiang‑Yun Zhao
    • Jing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3351-3358
    |
    Published online on: June 2, 2015
       https://doi.org/10.3892/mmr.2015.3873
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Abstract

The present study aimed to investigate the effects of endogenous hydrogen sulfide (H2S) on the expression levels of angiotensin II type 1 receptor (AGTR1) in a rat model of carbon tetrachloride (CCl4)‑induced hepatic fibrosis. A total of 56 Wistar rats were randomly divided into four groups: Normal control group, model group, sodium hydrosulfide (NaHS) group, and DL‑propargylglycine (PAG) group. Hepatic fibrosis was induced by CCl4. The rats in the PAG group were intraperitoneally injected with PAG, an inhibitor of cystathionine‑γ‑lyase (CSE). The rats in the NaHS group were intraperitoneally injected with NaHS. An equal volume of saline solution was intraperitoneally injected into both the control and model groups. All rats were sacrificed at week three or four following treatment. The serum levels of hyaluronidase (HA), laminin protein (LN), procollagen III (PcIII), and collagen IV (cIV) were detected using ELISA. The serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and albumin (ALB) were detected using an automatic biochemical analyzer. The liver mRNA expression levels of CSE were detected by reverse transcription‑quantitative polymerase chain reaction. The liver expression levels of AGTR1 and the plasma expression levels of H2S were detected using western blot analyses. The results indicated that the severity of hepatic fibrosis, the serum expression levels of HA, LN, PcIII, cIV, ALT, and AST, the liver expression levels of CSE and AGTR1, and the plasma expression levels of H2S were significantly higher in the PAG group, as compared with the model group (P<0.05). Conversely, the expression levels of ALB were significantly lower in the PAG group, as compared with the model group. In addition, the severity of hepatic fibrosis, the serum expression levels of HA, LN, PcIII, cIV, ALT, and AST, the liver expression levels of CSE and AGTR1, and the plasma expression levels of H2S were significantly lower in the NaHS group, as compared with the model group (P<0.05). These results suggest that endogenous H2S is associated with CCl4‑induced hepatic fibrosis in rats, and may exhibit anti‑fibrotic effects. Furthermore, H2S reduced the liver expression levels of AGTR1, which may be associated with the delayed progression of hepatic fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Fan HN, Chen NW, Shen WL, Zhao XY and Zhang J: Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis. Mol Med Rep 12: 3351-3358, 2015.
APA
Fan, H., Chen, N., Shen, W., Zhao, X., & Zhang, J. (2015). Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis. Molecular Medicine Reports, 12, 3351-3358. https://doi.org/10.3892/mmr.2015.3873
MLA
Fan, H., Chen, N., Shen, W., Zhao, X., Zhang, J."Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis". Molecular Medicine Reports 12.3 (2015): 3351-3358.
Chicago
Fan, H., Chen, N., Shen, W., Zhao, X., Zhang, J."Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis". Molecular Medicine Reports 12, no. 3 (2015): 3351-3358. https://doi.org/10.3892/mmr.2015.3873
Copy and paste a formatted citation
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Spandidos Publications style
Fan HN, Chen NW, Shen WL, Zhao XY and Zhang J: Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis. Mol Med Rep 12: 3351-3358, 2015.
APA
Fan, H., Chen, N., Shen, W., Zhao, X., & Zhang, J. (2015). Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis. Molecular Medicine Reports, 12, 3351-3358. https://doi.org/10.3892/mmr.2015.3873
MLA
Fan, H., Chen, N., Shen, W., Zhao, X., Zhang, J."Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis". Molecular Medicine Reports 12.3 (2015): 3351-3358.
Chicago
Fan, H., Chen, N., Shen, W., Zhao, X., Zhang, J."Endogenous hydrogen sulfide is associated with angiotensin II type 1 receptor in a rat model of carbon tetrachloride‑induced hepatic fibrosis". Molecular Medicine Reports 12, no. 3 (2015): 3351-3358. https://doi.org/10.3892/mmr.2015.3873
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