Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts

  • Authors:
    • Gen Kuroyanagi
    • Haruhiko Tokuda
    • Naohiro Yamamoto
    • Rie Matsushima‑Nishiwaki
    • Jun Mizutani
    • Osamu Kozawa
    • Takanobu Otsuka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu 501‑1194, Japan
  • Pages: 3849-3854
    |
    Published online on: June 3, 2015
       https://doi.org/10.3892/mmr.2015.3877
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Resveratrol is a naturally occurring polyphenol that possesses health‑related properties, and is predominantly found in grapes and berries. Bone morphogenetic protein‑4 (BMP‑4) stimulates osteocalcin synthesis via p38 mitogen‑activated protein (MAP) kinase in osteoblast‑like MC3T3‑E1 cells. The present study aimed to investigate the effects of resveratrol on BMP‑4‑induced osteoprotegerin (OPG) synthesis in MC3T3‑E1 cells. Resveratrol alone had no effect on OPG expression levels, but significantly enhanced BMP‑4‑induced OPG release. In addition, resveratrol markedly amplified the mRNA expression levels of BMP‑4‑induced OPG. SB203580 is an inhibitor of p38 MAP kinase, which was shown to suppress BMP‑4‑stimulated OPG release. BMP‑4‑induced phosphorylation of p38 MAP kinase was also enhanced by resveratrol. Furthermore, SB203580 significantly reduced the resveratrol‑induced amplification of BMP‑4‑stimulated OPG release. These results suggested that resveratrol was able to upregulate BMP‑4‑stimulated OPG synthesis via the amplification of p38 MAP kinase activity in osteoblasts.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Karsenty G and Wagner EF: Reaching a genetic and molecular understanding of skeletal development. Dev Cell. 2:389–406. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Boyce BF, Rosenberg E, de Papp AE and Duong le T: The osteoclast, bone remodelling and treatment of metabolic bone disease. Eur J Clin Invest. 42:1332–1341. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Parfitt AM: Targeted and nontargeted bone remodeling: Relationship to basic multicellular unit origination and progression. Bone. 30:5–7. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Simonet WS, Lacey DL, Dunstan CR, Kelley M, Chang MS, Lüthy R, Nguyen HQ, Wooden S, Bennett L and Boone T: Osteoprotegerin: A novel secreted protein involved in the regu lation of bone density. Cell. 89:309–319. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Mizuno A, Kanno T, Hoshi M, Shibata O, Yano K, Fujise N, Kinosaki M, Yamaguchi K, Tsuda E, Murakami A, et al: Transgenic mice overexpressing soluble osteoclast differen tiation factor (sODF) exhibit severe osteoporosis. J Bone Miner Metab. 20:337–344. 2002. View Article : Google Scholar

6 

Kwan Tat S, Padrines M, Théoleyre S, Heymann D and Fortun Y: IL 6, RANKL, TNF alpha/IL 1: Interrelations in bone resorption pathophysiology. Cytokine Growth Factor Rev. 15:49–60. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Miyazono K, Maeda S and Imamura T: BMP receptor signaling: Transcriptional targets, regulation of signals, and signaling cross talk. Cytokine Growth Factor Rev. 16:251–263. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Krause C, de Gorter DJJ, Karperien M and ten Dijke P: Signal trans duction cascades controlling osteoblast differentiation. Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. Rosen CJ: 7th. ASBMR; Washington: 2008, View Article : Google Scholar

9 

Moustakas A and Heldin CH: Non Smad TGF beta signals. J Cell Sci. 118:3573–3584. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Cai J, Pardali E, Sánchez Duffhues G and ten Dijke P: BMP signaling in vascular diseases. FEBS Lett. 586:1993–2002. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kozawa O, Hatakeyama D and Uematsu T: Divergent regulation by p44/p42 MAP kinase and p38 MAP kinase of bone morpho genetic protein 4 stimulated osteocalcin synthesis in osteoblasts. J Cell Biochem. 84:583–589. 2002. View Article : Google Scholar

12 

Tokuda H, Hatakeyama D, Shibata T, Akamatsu S, Oiso Y and Kozawa O: p38 MAP kinase regulates BMP-4-stimulated VEGF synthesis via p70 S6 kinase in osteoblasts. Am J Physiol Endocrinol Metab. 284:E1202–E1209. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Hofbauer LC, Dunstan CR, Spelsberg TC, Riggs BL and Khosla S: Osteoprotegerin production by human osteoblast lineage cells is stimulated by vitamin D, bone morpho genetic protein 2, and cytokines. Biochem Biophys Res Commun. 250:776–781. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Jankun J, Selman SH, Swiercz R and Skrzypczak Jankun E: Why drinking green tea could prevent cancer. Nature. 387:5611997. View Article : Google Scholar : PubMed/NCBI

15 

Harborne JB and Williams CA: Advances in flavonoid research since 1992. Phytochemistry. 55:481–504. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez Lluch G, Lewis K, et al: Resveratrol improves health and survival of mice on a high calorie diet. Nature. 444:337–342. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Renaud S and de Lorgeril M: Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet. 339:1523–1526. 1992. View Article : Google Scholar : PubMed/NCBI

18 

Mizutani K, Ikeda K, Kawai Y and Yamori Y: Resveratrol stimulates the proliferation and differentiation of osteoblastic MC3T3 E1 cells. Biochem Biophys Res Commun. 253:859–863. 1998. View Article : Google Scholar

19 

Sudo H, Kodama HA, Amagai Y, Yamamoto S and Kasai S: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96:191–198. 1983. View Article : Google Scholar : PubMed/NCBI

20 

Kozawa O, Tokuda H, Miwa M, Kotoyori J and Oiso Y: Cross talk regulation between cyclic AMP production and phosphoinositide hydrolysis induced by prostaglandin E2 in osteoblast-like cells. Exp Cell Res. 198:130–134. 1992. View Article : Google Scholar : PubMed/NCBI

21 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

22 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress induced synthesis of hsp27 and alpha B crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Simpson DA, Feeney S, Boyle C and Stitt AW: Retinal VEGF mRNA measured by SYBR green I fluorescence: A versatile approach to quantitative PCR. Mol Vis. 6:178–183. 2000.PubMed/NCBI

24 

Cuenda A, Rouse J, Doza YN, Meier R, Cohen P, Gallagher TF, Young PR and Lee JC: SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin 1. FEBS Lett. 364:229–233. 1995. View Article : Google Scholar : PubMed/NCBI

25 

Kondo A, Otsuka T, Kuroyanagi G, Yamamoto N, Matsushima Nishiwaki R, Mizutani J, Kozawa O and Tokuda H: Resveratrol inhibits BMP-4-stimulated VEGF synthesis in osteo blasts: Suppression of S6 kinase. Int J Mol Med. 33:1013–1018. 2014.PubMed/NCBI

26 

Blander G and Guarente L: The Sir2 family of protein deacetylases. Annu Rev Biochem. 73:417–435. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Koo SH and Montminy M: In vino veritas: a tale of two sirt1s? Cell. 127:1091–1093. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kuroyanagi G, Tokuda H, Yamamoto N, Matsushima‑Nishiwaki R, Mizutani J, Kozawa O and Otsuka T: Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts. Mol Med Rep 12: 3849-3854, 2015.
APA
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., & Otsuka, T. (2015). Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts. Molecular Medicine Reports, 12, 3849-3854. https://doi.org/10.3892/mmr.2015.3877
MLA
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., Otsuka, T."Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts". Molecular Medicine Reports 12.3 (2015): 3849-3854.
Chicago
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., Otsuka, T."Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts". Molecular Medicine Reports 12, no. 3 (2015): 3849-3854. https://doi.org/10.3892/mmr.2015.3877
Copy and paste a formatted citation
x
Spandidos Publications style
Kuroyanagi G, Tokuda H, Yamamoto N, Matsushima‑Nishiwaki R, Mizutani J, Kozawa O and Otsuka T: Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts. Mol Med Rep 12: 3849-3854, 2015.
APA
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., & Otsuka, T. (2015). Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts. Molecular Medicine Reports, 12, 3849-3854. https://doi.org/10.3892/mmr.2015.3877
MLA
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., Otsuka, T."Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts". Molecular Medicine Reports 12.3 (2015): 3849-3854.
Chicago
Kuroyanagi, G., Tokuda, H., Yamamoto, N., Matsushima‑Nishiwaki, R., Mizutani, J., Kozawa, O., Otsuka, T."Resveratrol amplifies BMP‑4‑stimulated osteoprotegerin synthesis via p38 MAP kinase in osteoblasts". Molecular Medicine Reports 12, no. 3 (2015): 3849-3854. https://doi.org/10.3892/mmr.2015.3877
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team