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Article

Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure

  • Authors:
    • Yong Li
    • Xiaohong Liu
    • Shouyue Huang
    • Ping Huang
    • Yisheng Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Tongren Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200336, P.R. China, Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200025, P.R. China, Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200025, P.R. China
  • Pages: 3993-3998
    |
    Published online on: June 3, 2015
       https://doi.org/10.3892/mmr.2015.3882
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Abstract

The A2A receptor (A2AR) antagonist has been considered as an attractive option to improve the treatment of neurological disorders, and the function of A2AR antagonist may inhibit the release of glutamate and prevent neuron damage. The aim of the present study was to investigate whether SCH442416 can modulate the glutamate uptake in retinal Müller cells under increased hydrostatic pressure. The levels of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) were assessed in retinal Müller cells under 40 mmHg pressure for 24 h using reverse transcription‑quantitative polymerase chain reaction and western blotting, and a glutamate uptake assay was performed using a scintillation counting method. Following treatment of the Müller cells with 100 nM SCH442416 under 40 mmHg pressure for 24 h, the mRNA and protein expression levels of GS and GLAST, and glutamate uptake activity were investigated. Under 40 mmHg pressure, the expression levels of GS and GLAST in the Müller cells, and glutamate uptake activity were significantly reduced. Treatment with SCH442416 significantly ameliorated the decreased expression levels of GS and GLAST, and improved the glutamate uptake activity in the retinal Müller cells exposed to 40 mmHg pressure, resulting in increased expression levels of GS and GLAST, and increased glutamate uptake activity in the Müller cells under pressure. These results suggested that SCH442416 may be a potential candidate as a beneficial neuroprotective agent for the treatment of glaucoma by accelerating the clearance of extracellular glutamate.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Liu X, Huang S, Huang P and Zhong Y: Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure. Mol Med Rep 12: 3993-3998, 2015.
APA
Li, Y., Liu, X., Huang, S., Huang, P., & Zhong, Y. (2015). Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure. Molecular Medicine Reports, 12, 3993-3998. https://doi.org/10.3892/mmr.2015.3882
MLA
Li, Y., Liu, X., Huang, S., Huang, P., Zhong, Y."Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure". Molecular Medicine Reports 12.3 (2015): 3993-3998.
Chicago
Li, Y., Liu, X., Huang, S., Huang, P., Zhong, Y."Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure". Molecular Medicine Reports 12, no. 3 (2015): 3993-3998. https://doi.org/10.3892/mmr.2015.3882
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Liu X, Huang S, Huang P and Zhong Y: Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure. Mol Med Rep 12: 3993-3998, 2015.
APA
Li, Y., Liu, X., Huang, S., Huang, P., & Zhong, Y. (2015). Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure. Molecular Medicine Reports, 12, 3993-3998. https://doi.org/10.3892/mmr.2015.3882
MLA
Li, Y., Liu, X., Huang, S., Huang, P., Zhong, Y."Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure". Molecular Medicine Reports 12.3 (2015): 3993-3998.
Chicago
Li, Y., Liu, X., Huang, S., Huang, P., Zhong, Y."Effect of SCH442416 on glutamate uptake in retinal Müller cells at increased hydrostatic pressure". Molecular Medicine Reports 12, no. 3 (2015): 3993-3998. https://doi.org/10.3892/mmr.2015.3882
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