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Article

Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs

  • Authors:
    • Keran Song
    • Tao Gu
    • Feng Shuang
    • Jiaguang Tang
    • Dongfeng Ren
    • Jiang Qin
    • Shuxun Hou
  • View Affiliations / Copyright

    Affiliations: Institute of Orthopaedics, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, P.R. China, Department of Orthopedic Surgery, Navy General Hospital, Beijing 100048, P.R. China, Department of Orthopedic Surgery, The 94th Hospital of Chinese PLA, Nanchang, Jiangxi 330002, P.R. China
  • Pages: 4664-4668
    |
    Published online on: June 8, 2015
       https://doi.org/10.3892/mmr.2015.3895
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Abstract

Patients with degenerative disc disease (DDD) experience serious clinical symptoms, including chronic low back pain. A series of therapies have been developed to treat DDD, including physical therapy and surgical treatment. However, the therapeutic effect of such treatments has remained insufficient. Recently, stem cell‑based therapy, in which stem cells are injected into the nucleus pulposus in degenerated intervertebral disc tissue, has appeared to be effective in the treatment of DDD. In the present study, the effect of adipose‑derived stem cells on degenerated nucleus pulposus cells was investigated using a co‑culture system to evaluate the biological activity of degenerated nucleus pulposus cells. Human degenerated nucleus pulposus tissue was obtained from surgical specimens and the adipose‑derived stem cells were derived from adipose tissue. The degenerated nucleus pulposus cells were cultured in a mono‑culture or in a co‑culture with adipose‑derived stem cells using 0.4‑µm Transwell inserts. The results indicated that adipose‑derived stem cells were able to stimulate matrix synthesis and the cell proliferation of degenerated nucleus pulposus cells, promoting the restoration of nucleus pulposus cells in the degenerated intervertebral disc.
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Copy and paste a formatted citation
Spandidos Publications style
Song K, Gu T, Shuang F, Tang J, Ren D, Qin J and Hou S: Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Mol Med Rep 12: 4664-4668, 2015.
APA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., & Hou, S. (2015). Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Molecular Medicine Reports, 12, 4664-4668. https://doi.org/10.3892/mmr.2015.3895
MLA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12.3 (2015): 4664-4668.
Chicago
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12, no. 3 (2015): 4664-4668. https://doi.org/10.3892/mmr.2015.3895
Copy and paste a formatted citation
x
Spandidos Publications style
Song K, Gu T, Shuang F, Tang J, Ren D, Qin J and Hou S: Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Mol Med Rep 12: 4664-4668, 2015.
APA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., & Hou, S. (2015). Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs. Molecular Medicine Reports, 12, 4664-4668. https://doi.org/10.3892/mmr.2015.3895
MLA
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12.3 (2015): 4664-4668.
Chicago
Song, K., Gu, T., Shuang, F., Tang, J., Ren, D., Qin, J., Hou, S."Adipose-derived stem cells improve the viability of nucleus pulposus cells in degenerated intervertebral discs". Molecular Medicine Reports 12, no. 3 (2015): 4664-4668. https://doi.org/10.3892/mmr.2015.3895
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