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Expression of HAX-1 in colorectal cancer and its role in cancer cell growth

  • Authors:
    • Xiaolan Li
    • Jianwu Jiang
    • Rui Yang
    • Xiangshang Xu
    • Fayong Hu
    • Anding Liu
    • Deding Tao
    • Yan Leng
    • Junbo Hu
    • Jianping Gong
    • Xuelai Luo
  • View Affiliations / Copyright

    Affiliations: Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China, Experimental Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 4071-4078
    |
    Published online on: June 11, 2015
       https://doi.org/10.3892/mmr.2015.3905
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Abstract

Colorectal cancer (CRC) is one of the most common types of cancer worldwide. Hematopoietic cell‑specific protein 1‑associated protein X‑1 (HAX‑1) has been found to be involved in several types of cancer. However, the role of HAX‑1 in CRC remains to be elucidated. The aim of the present study was to investigate whether the expression of HAX‑1 is associated with the progression of CRC, and to determine the effects of HAX‑1 on the apoptosis and proliferation of CRC cells. Tumor tissues and adjacent noncancerous tissues were collected from 60 patients with CRC, following the provision of informed consent. The expression levels of HAX‑1 and the association with clinical and pathological characteristics were then analyzed. The expression levels of HAX‑1 were significantly higher in the cancerous tissues from the patients with CRC, particularly in tissues of an advanced stage of cancer. In addition, HAX‑1 expression was associated with malignant progression and poor prognosis. Furthermore, SW480 CRC cells, overexpressing HAX‑1, exhibited increased resistance to camptothecin in vitro, and promoted proliferation in vitro and in vivo. By contrast, HAX‑1 knockdown significantly decreased the proliferation. In addition, the expression levels of ki‑67 and phosphorylated‑akt were inhibited following HAX‑1 knockdown. In conclusion, the expression levels of HAX‑1 were increased in cancerous tissue from patients with CRC, and were associated with progression of the disease. These results suggested that HAX‑1 may contribute to chemotherapy resistance and malignant progression in CRC.
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1 

Siegel R, Desantis C and Jemal A: Colorectal cancer statistics, 2014. CA Cancer J Clin. 64:104–117. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Eilstein D, Hedelin G and Schaffer P: Incidence of colorectal cancer in Bas-Rhin, trend and prediction in 2009. Bull Cancer. 87:595–599. 2000.In French. PubMed/NCBI

4 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Adam R, Haller DG, Poston G, et al: Toward optimized front-line therapeutic strategies in patients with metastatic colorectal cancer-an expert review from the International Congress on Anti-Cancer Treatment (ICACT) 2009. Annals of Oncology. 21:1579–1584. 2010. View Article : Google Scholar

6 

Hatakeyama S, Watanabe M, Fujii Y and Nakayama KI: Targeted destruction of c-Myc by an engineered ubiquitin ligase suppresses cell transformation and tumor formation. Cancer Res. 65:7874–7879. 2005.PubMed/NCBI

7 

Suzuki Y, Demoliere C, Kitamura D, Takeshita H, Deuschle U and Watanabe T: HAX-1, a novel intracellular protein, localized on mitochondria, directly associates with HS1, a substrate of Src family tyrosine kinases. J Immunol. 158:2736–2744. 1997.PubMed/NCBI

8 

Chao JR, Parganas E, Boyd K, Hong CY, Opferman JT and Ihle JN: Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature. 452:98–102. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Wyllie AH, Kerr JF, Macaskill IA and Currie AR: Adrenocortical cell deletion: the role of ACTH. J Pathol. 111:85–94. 1973. View Article : Google Scholar : PubMed/NCBI

10 

Radhika V, Onesime D, Ha JH and Dhanasekaran N: Galpha13 stimulates cell migration through cortactin-interacting protein Hax-1. J Biol Chem. 279:49406–49413. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Yedavalli VS, Shih HM, Chiang YP, et al: Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J Virol. 79:13735–13746. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Vafiadaki E, Sanoudou D, Arvanitis DA, Catino DH, Kranias EG and Kontrogianni-Konstantopoulos A: Phospholamban interacts with HAX-1, a mitochondrial protein with anti-apoptotic function. J Mol Biol. 367:65–79. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Vafiadaki E, Arvanitis DA, Pagakis SN, et al: The anti-apoptotic protein HAX-1 interacts with SERCA2 and regulates its protein levels to promote cell survival. Mol Biol Cell. 20:306–318. 2009. View Article : Google Scholar :

14 

Sun SJ, Feng L, Zhao GQ and Dong ZM: HAX-1 promotes the chemoresistance, invasion, and tumorigenicity of esophageal squamous carcinoma cells. Dig Dis Sci. 57:1838–1846. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Banerjee A, Saito K, Meyer K, et al: Hepatitis C virus core protein and cellular protein HAX-1 promote 5-fluorouracil-mediated hepatocyte growth inhibition. J Virol. 83:9663–9671. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Luo X, Li Z, Li X, et al: hSav1 interacts with HAX1 and attenuates its anti-apoptotic effects in MCF-7 breast cancer cells. Int J Mol Med. 28:349–355. 2011.PubMed/NCBI

17 

Li X, Luo X, Li Z, et al: Screening of binding proteins that interact with human Salvador 1 in a human fetal liver cDNA library by the yeast two-hybrid system. Mol Biol Rep. 39:8225–8230. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Jiang J, Liu L, Li X, Tao D, Hu J and Qin J: Defining the restriction point in normal asynchronous human peripheral blood lymphocytes. Cytometry A. 83:944–951. 2013.PubMed/NCBI

19 

Lees DM, Hart IR and Marshall JF: Existence of multiple isoforms of HS1-associated protein X-1 in murine and human tissues. J Mol Biol. 379:645–655. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Carlsson G, van't Hooft I, Melin M, et al: Central nervous system involvement in severe congenital neutropenia: neurological and neuropsychological abnormalities associated with specific HAX1 mutations. J Intern Med. 264:388–400. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Han Y, Chen YS, Liu Z, et al: Overexpression of HAX-1 protects cardiac myocytes from apoptosis through caspase-9 inhibition. Circ Res. 99:415–423. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Fadeel B and Grzybowska E: HAX-1: a multifunctional protein with emerging roles in human disease. Biochim Biophys Acta. 1790:1139–1148. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Rhodes DR, Yu J, Shanker K, et al: ONCOMINE: a cancer microarray database and integrated data-mining platform. Neoplasia. 6:1–6. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Trebinska A, Rembiszewska A, Ciosek K, et al: HAX-1 overexpression, splicing and cellular localization in tumors. BMC Cancer. 10:762010. View Article : Google Scholar : PubMed/NCBI

25 

Wei XJ, Li SY, Yu B, Chen G, Du JF and Cai HY: Expression of HAX-1 in human colorectal cancer and its clinical significance. Tumour Biol. 35:1411–1415. 2014. View Article : Google Scholar

26 

Ashhab Y, Alian A, Polliack A, Panet A and Ben Yehuda D: Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distribution pattern. FEBS Lett. 495:56–60. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Li M, Tang Y, Zang W, et al: Analysis of HAX-1 gene expression in esophageal squamous cell carcinoma. Diagn Pathol. 8:472013. View Article : Google Scholar : PubMed/NCBI

28 

Kaufmann SH and Gores GJ: Apoptosis in cancer: cause and cure. Bioessays. 22:1007–1017. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Schmitt CA: Senescence, apoptosis and therapy - cutting the lifelines of cancer. Nat Rev Cancer. 3:286–295. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Nachmias B, Ashhab Y, Bucholtz V, et al: Caspase-mediated cleavage converts Livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma. Cancer Res. 63:6340–6349. 2003.PubMed/NCBI

31 

Deng W, Wang DA, Gosmanova E, Johnson LR and Tigyi G: LPA protects intestinal epithelial cells from apoptosis by inhibiting the mitochondrial pathway. Am J Physiol Gastrointest Liver Physiol. 284:G821–G829. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Jiang J, Yang R, Xu X, Hu F, Liu A, Tao D, Leng Y, Hu J, Gong J, Gong J, et al: Expression of HAX-1 in colorectal cancer and its role in cancer cell growth. Mol Med Rep 12: 4071-4078, 2015.
APA
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A. ... Luo, X. (2015). Expression of HAX-1 in colorectal cancer and its role in cancer cell growth. Molecular Medicine Reports, 12, 4071-4078. https://doi.org/10.3892/mmr.2015.3905
MLA
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A., Tao, D., Leng, Y., Hu, J., Gong, J., Luo, X."Expression of HAX-1 in colorectal cancer and its role in cancer cell growth". Molecular Medicine Reports 12.3 (2015): 4071-4078.
Chicago
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A., Tao, D., Leng, Y., Hu, J., Gong, J., Luo, X."Expression of HAX-1 in colorectal cancer and its role in cancer cell growth". Molecular Medicine Reports 12, no. 3 (2015): 4071-4078. https://doi.org/10.3892/mmr.2015.3905
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Jiang J, Yang R, Xu X, Hu F, Liu A, Tao D, Leng Y, Hu J, Gong J, Gong J, et al: Expression of HAX-1 in colorectal cancer and its role in cancer cell growth. Mol Med Rep 12: 4071-4078, 2015.
APA
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A. ... Luo, X. (2015). Expression of HAX-1 in colorectal cancer and its role in cancer cell growth. Molecular Medicine Reports, 12, 4071-4078. https://doi.org/10.3892/mmr.2015.3905
MLA
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A., Tao, D., Leng, Y., Hu, J., Gong, J., Luo, X."Expression of HAX-1 in colorectal cancer and its role in cancer cell growth". Molecular Medicine Reports 12.3 (2015): 4071-4078.
Chicago
Li, X., Jiang, J., Yang, R., Xu, X., Hu, F., Liu, A., Tao, D., Leng, Y., Hu, J., Gong, J., Luo, X."Expression of HAX-1 in colorectal cancer and its role in cancer cell growth". Molecular Medicine Reports 12, no. 3 (2015): 4071-4078. https://doi.org/10.3892/mmr.2015.3905
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