Targeted sequencing of cancer-associated genes in hepatocellular carcinoma using next generation sequencing

Lu,Jianguo; Yin,Jikai; Dong,Rui; Yang,Tao; Yuan,Lijuan; Zang,Li; Xu,Cheng; Peng,Bo; Zhao,Jiangman; Du,Xilin

doi:10.3892/mmr.2015.3952

September-2015 Volume 12 Issue 3

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September-2015 Volume 12 Issue 3

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Article

Targeted sequencing of cancer-associated genes in hepatocellular carcinoma using next generation sequencing

Authors:
- Jianguo Lu
- Jikai Yin
- Rui Dong
- Tao Yang
- Lijuan Yuan
- Li Zang
- Cheng Xu
- Bo Peng
- Jiangman Zhao
- Xilin Du
View Affiliations / Copyright

Affiliations: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Medicine, Shanghai Zhangjiang Translational Medicine Research Center, Shanghai 201203, P.R. China
Pages: 4678-4682
|
Published online on: June 17, 2015

https://doi.org/10.3892/mmr.2015.3952
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Abstract

Liver cancer is one of the most common causes of cancer-associated mortality. Hepatocellular carcinoma (HCC) is the major histological subtype among types of primary liver cancer. China is an area of high incidence of HCC, and >50% of the cases of HCC worldwide are in China. At present, the mechanism underlying the development of HCC remains to be fully elucidated, and previous studies have predominantly focused on HCC in southern and eastern China, with molecular data of the HCC cases in Western China remains limited. In the present study, a panel of 372 cancer‑associated genes were screened using a next generation sequencing platform, which included a total of 12 cases from western China. The results confirmed mutations in previously identified HCC drivers, including p53 and Kras. Additionally, mutations in several cancer genes, which had not been previously associated with HCC, were identified, including RUNX1 and JAK3. The present study provided a mutation spectrum of HCC tissue in cases from western China, assisting in the investigation of the mechanism of liver carcinogenesis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Targeted sequencing of cancer-associated genes in hepatocellular carcinoma using next generation sequencing

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Abstract

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