Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2015 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway

  • Authors:
    • Zhuang Hu
    • Lingling Xie
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Thyroid Surgery, Huaihe Hospital of Henan University, Kaifeng, Henan 475000, P.R. China, Operation Room, Zhangqiu City Hospital of Traditional Chinese Medicine, Zhangqiu, Shandong 250200, P.R. China
  • Pages: 4634-4639
    |
    Published online on: June 25, 2015
       https://doi.org/10.3892/mmr.2015.3997
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

LIM homeobox domain 6 (LHX6), a member of the LHX family of proteins, has been implicated in cancer development. However, the involvement of LHX6 in the development of breast cancer remains unclear. In the present study, the epigenetic regulation, biological function and associated molecular mechanisms of LHX6 in breast cancer were analyzed. The expression levels of LHX6 were demonstrated to be markedly decreased in breast cancer tissues and cell lines. In addition, it was found that increased LHX6 expression in breast cancer cell lines inhibited cell proliferation and invasion. Furthermore, increased LHX6 expression significantly decreased the expression of β‑catenin in MDA‑MB‑231 breast cancer cells, and small interfering RNA‑β‑catenin enhanced LHX6‑induced inhibition of cell proliferation and invasion in MDA‑MB‑231 breast cancer cells. These results indicate that LHX6 inhibits breast cancer cell growth and invasion through suppression of the Wnt/β‑catenin signaling pathway. Thus, the present study provides a novel insight into the underlying mechanism of tumorigenesis in breast cancer, indicating the therapeutic potential of LHX6 in the treatment of breast cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Siegel R, Ward E, Hao Y, Xu J and Thun MJ: Cancer statistics, 2009. CA Cancer J Clin. 59:225–249. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics. 2002. CA Cancer J Clin. 55:74–108. 2002. View Article : Google Scholar

4 

Voduc KD, Cheang MC, Tyldesley S, Gelmon K, Nielsen TO and Kennecke H: Breast cancer subtypes and the risk of local and regional relapse. J Clin Oncol. 28:1684–1691. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Hobert O and Westphal H: Functions of LIM-homeobox genes. Trends Genet. 16:75–83. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Hung TM, Hu RH, Ho CM, Chiu YL, Lee JL, Jeng YM, Shih DT and Lee PH: Downregulation of alpha-fetoprotein expression by LHX4: A critical role in hepatocarcinogenesis. Carcinogenesis. 32:1815–1823. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Kuzmanov A, Hopfer U, Marti P, Meyer-Schaller N, Yilmaz M and Christofori G: LIM-homeobox gene 2 promotes tumor growth and metastasis by inducing autocrine and paracrine PDGF-B signaling. Mol Oncol. 8:401–416. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Vladimirova V, Mikeska T, Waha A, Soerensen N, Xu J, Reynolds PC and Pietsch T: Aberrant methylation and reduced expression of LHX9 in malignant gliomas of childhood. Neoplasia. 11:700–711. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Grigoriou M, Tucker AS, Sharpe PT and Pachnis V: Expression and regulation of Lhx6 and Lhx7, a novel subfamily of LIM homeodomain encoding genes, suggests a role in mammalian head development. Development. 125:2063–2074. 1998.PubMed/NCBI

10 

Alifragis P, Liapi A and Parnavelas JG: Lhx6 regulates the migration of cortical interneurons from the ventral telencephalon but does not specify their GABA phenotype. J Neurosci. 24:5643–5648. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Liodis P, Denaxa M, Grigoriou M, Akufo-Addo C, Yanagawa Y and Pachnis V: Lhx6 activity is required for the normal migration and specification of cortical interneuron subtypes. J Neurosci. 27:3078–3089. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Estécio M, Youssef EM, Rahal P, Fukuyama EE, Góis-Filho J, Maniglia JV, Goloni-Bertollo EM, Issa JU and Tajara EH: LHX6 is a sensitive methylation marker in head and neck carcinomas. Oncogene. 25:5018–5026. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Liu WB, Jiang X, Han F, Li YH, Chen HQ, Liu Y, Cao J and Liu JY: LHX6 acts as a novel potential tumour suppressor with epigenetic inactivation in lung cancer. Cell Death Dis. 4:e8822013. View Article : Google Scholar : PubMed/NCBI

14 

Kwon SY and Bae Y: Reclassification of mammary carcinomas with neuroendocrine features in accordance with neuroendocrine morphologies in the revised World Health Organization classification. Histopathology. 66:755–756. 2015. View Article : Google Scholar

15 

Winchell CJ and Jacobs DK: Expression of the Lhx genes apterous and lim1 in an errant polychaete: Implications for bilaterian appendage evolution, neural development and muscle diversification. Evodevo. 4:42013. View Article : Google Scholar

16 

Jung S, Jeong D, Kim J, Yi L, Koo K, Lee J, Kim CJ, Kim CH, An S, Yang Y, et al: Epigenetic regulation of the potential tumor suppressor gene, hLHX6.1, in human cervical cancer. Int J Oncol. 38:859–869. 2011.PubMed/NCBI

17 

Choi Y, Ballow DJ, Xin Y and Rajkovic A: Lim homeobox gene, lhx8, is essential for mouse oocyte differentiation and survival. Biol Reprod. 79:442–449. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Anastas JN and Moon RT: WNT signalling pathways as therapeutic targets in cancer. Nat Rev Cancer. 13:11–26. 2013. View Article : Google Scholar

19 

Yao H, Ashihara E and Maekawa T: Targeting the Wnt/β-catenin signaling pathway in human cancers. Expert Opin Ther Targets. 15:873–887. 2011. View Article : Google Scholar : PubMed/NCBI

20 

MacDonald BT, Tamai K and He X: Wnt/beta-catenin signaling: Components, mechanisms and diseases. Dev Cell. 17:9–26. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Fodde R and Brabletz T: Wnt/beta-catenin signaling in cancer stemness and malignant behavior. Curr Opin Cell Biol. 19:150–158. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Samowitz WS, Powers MD, Spirio LN, Nollet F, van Roy F and Slattery ML: Beta-catenin mutations are more frequent in small colorectal adenomas than in larger adenomas and invasive carcinomas. Cancer Res. 59:1442–1444. 1999.PubMed/NCBI

23 

Scholer-Dahirel A, Schlabach MR, Loo A, Bagdasarian L, Meyer R, Guo R, Woolfenden S, Yu KK, Markovits J, Killary K, et al: Maintenance of adenomatous polyposis coli (APC)-mutant colorectal cancer is dependent on Wnt/beta-catenin signaling. Proc Natl Acad Sci USA. 108:17135–17140. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Tang X, Zheng D, Hu P, Zeng Z, Li M, Tucker L, Monahan R, Resnick MB, Liu M and Ramratnam B: Glycogen synthase kinase 3 beta inhibits microRNA-183-96-182 cluster via the β-Catenin/TCF/LEF-1 pathway in gastric cancer cells. Nucleic Acids Res. 42:2988–2998. 2014. View Article : Google Scholar :

25 

Cheyette BN, Waxman JS, Miller JR, Takemaru K, Sheldahl LC, Khlebtsova N, Fox EP, Earnest T and Moon RT: Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation. Dev Cell. 2:449–461. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Zhang Z, Gutierrez D, Li X, Bidlack F, Cao H, Wang J, Andrade K, Margolis HC and Amendt BA: The LIM Homeodomain transcription factor LHX6 A trancriptional repressor that interfacts with pituitary homebox 2 (PITX2) to regulate odontogenesis. J Biol Chem. 288:2485–2500. 2013. View Article : Google Scholar :

27 

Vadlamudi U, Espinoza HM, Ganga M, Martin DM, Liu X, Engelhardt JF and Amendt BA: PITX2, beta-catenin and LEF-1 interact to synergistically regulate the LEF-1 promoter. J Cell Sci. 118:1129–1137. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hu Z and Xie L: LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway. Mol Med Rep 12: 4634-4639, 2015.
APA
Hu, Z., & Xie, L. (2015). LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway. Molecular Medicine Reports, 12, 4634-4639. https://doi.org/10.3892/mmr.2015.3997
MLA
Hu, Z., Xie, L."LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 12.3 (2015): 4634-4639.
Chicago
Hu, Z., Xie, L."LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 12, no. 3 (2015): 4634-4639. https://doi.org/10.3892/mmr.2015.3997
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Z and Xie L: LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway. Mol Med Rep 12: 4634-4639, 2015.
APA
Hu, Z., & Xie, L. (2015). LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway. Molecular Medicine Reports, 12, 4634-4639. https://doi.org/10.3892/mmr.2015.3997
MLA
Hu, Z., Xie, L."LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 12.3 (2015): 4634-4639.
Chicago
Hu, Z., Xie, L."LHX6 inhibits breast cancer cell proliferation and invasion via repression of the Wnt/β‑catenin signaling pathway". Molecular Medicine Reports 12, no. 3 (2015): 4634-4639. https://doi.org/10.3892/mmr.2015.3997
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team