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Article Open Access

Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact

  • Authors:
    • Li‑Na Zhou
    • Xiao‑Jun Cui
    • Kai‑Xin Su
    • Xiao‑Hong Wang
    • Jin‑Hua Guo
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4931-4938
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/mmr.2015.4016
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Abstract

In order to examine how implanted bone marrow stromal cells (BMSCs) encourage peripheral nerve regeneration, the present study investigated the interaction of BMSCs and Schwann cells (SCs) using an indirect in vitro co‑culture model. SCs and BMSCs were obtained from adult Sprague‑Dawley rats. The passaged BMSCs were CD29‑ and CD44‑positive but CD45‑negative and were co‑cultured with the primary SCs using a Millicell system, which allows BMSCs and SCs to grow in the same culture medium but without direct contact. Expression of the typical SC markers S‑100 and glial fibrillary acidic protein (GFAP) of the treated BMSCs as well as the proliferation capacity of the co‑cultured SCs was evaluated by immunocytochemical staining on the 3rd and 5th day of co‑culture. Immunocytochemical staining showed that >75% of the BMSCs in the indirect co‑culture model were GFAP‑ and S‑100‑positive on the 3rd and 5th day after co‑culture, as opposed to <5% of the BMSCs in the control group. On the 3rd day after co‑culture, only a few co‑cultured BMSCs showed the typical SC‑like morphology, while most BMSCs still kept their native appearance. By contrast, on the 5th day after co‑culture, almost all of the co‑cultured BMSCs appeared with the typical SC‑like morphology. Furthermore, 70.71% of the SCs in the indirect co‑culture model were S‑100‑positive on the 5th day of co‑culture, as opposed to >30.43% of the SCs in the control group. These results indicated that BMSCs may interact synergistically with SCs with regard to promoting peripheral nerve regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou LN, Cui XJ, Su KX, Wang XH and Guo JH: Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact. Mol Med Rep 12: 4931-4938, 2015.
APA
Zhou, L., Cui, X., Su, K., Wang, X., & Guo, J. (2015). Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact. Molecular Medicine Reports, 12, 4931-4938. https://doi.org/10.3892/mmr.2015.4016
MLA
Zhou, L., Cui, X., Su, K., Wang, X., Guo, J."Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact". Molecular Medicine Reports 12.4 (2015): 4931-4938.
Chicago
Zhou, L., Cui, X., Su, K., Wang, X., Guo, J."Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact". Molecular Medicine Reports 12, no. 4 (2015): 4931-4938. https://doi.org/10.3892/mmr.2015.4016
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou LN, Cui XJ, Su KX, Wang XH and Guo JH: Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact. Mol Med Rep 12: 4931-4938, 2015.
APA
Zhou, L., Cui, X., Su, K., Wang, X., & Guo, J. (2015). Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact. Molecular Medicine Reports, 12, 4931-4938. https://doi.org/10.3892/mmr.2015.4016
MLA
Zhou, L., Cui, X., Su, K., Wang, X., Guo, J."Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact". Molecular Medicine Reports 12.4 (2015): 4931-4938.
Chicago
Zhou, L., Cui, X., Su, K., Wang, X., Guo, J."Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co‑culture system in vitro without intercellular contact". Molecular Medicine Reports 12, no. 4 (2015): 4931-4938. https://doi.org/10.3892/mmr.2015.4016
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