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Article Open Access

Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration

  • Authors:
    • Peng Hu
    • Bo Feng
    • Guanglin Wang
    • Bin Ning
    • Tanghong Jia
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Jinan Central Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Department of Neurology, Hospital Affiliated to Binzhou Medical University, Binzhou, Shandong 256603, P.R. China, Department of Spine, Hospital Affiliated to Binzhou Medical University, Binzhou, Shandong 256603, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4925-4930
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/mmr.2015.4022
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Abstract

The present study aimed to explore the underlying mechanism of the development of intervertebral disc degeneration (IDD) by bioinformatics based on microarray datasets. GSE 19943 and GSE 34095 datasets downloaded from Gene Expression Omnibus data were used to screen the differentially expressed genes (DEGs) in IDD. The correlation between microRNAs and target genes was investigated using different algorithms. The underlying molecular mechanisms of the target genes were then explored using Kyoto Encyclopedia of Genes and Genomes pathway and Gene Ontology function enrichment analysis. A total of 9 differentially expressed microRNAs, including 3 down‑ and 6 upregulated microRNAs and 850 DEGs were identified in tissue from patients with IDD. Two regulation networks of the target genes by microRNAs were constructed, including 33 upregulated microRNA‑target gene pairs and 4 downregulated microRNA‑target gene pairs. Certain target genes had been demonstrated to be involved in IDD progression via various pathways, including in the cell cycle and pathways in cancer. In addition, two important microRNAs (microRNA‑222 and microRNA‑589) were identified that were pivotal for the development of IDD, and their target genes, CDKNAB and SMAD4. In conclusion, a comprehensive miRNA‑target gene regulatory network was constructed, which was found to be important in IDD progression.
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1 

Kalichman L and Hunter DJ: The genetics of intervertebral disc degeneration. Familial predisposition and heritability estimation. Joint Bone Spine. 75:383–387. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Roberts S, Evans H, Trivedi J and Menage J: Histology and pathology of the human intervertebral disc. J Bone Joint Surg Am. 2:10–14. 2006. View Article : Google Scholar

3 

Adams MA, Freeman BJ, Morrison HP, Nelson IW and Dolan P: Mechanical initiation of intervertebral disc degeneration. Spine (Phila Pa 1976). 25:1625–1636. 2000. View Article : Google Scholar

4 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Khvorova A, Reynolds A and Jayasena SD: Functional siRNAs and miRNAs exhibit strand bias. Cell. 115:209–216. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Zhao B, Yu Q, Li H, Guo X and He X: Characterization of microRNA expression profiles in patients with intervertebral disc degeneration. Int J Mol Med. 33:43–50. 2014.

7 

Wang HQ, Yu XD, Liu ZH, Cheng X, Samartzis D, Jia LT, Wu SX, Huang J, Chen J and Luo ZJ: Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3. J Pathol. 225:232–242. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Abouheif MM, Nakasa T, Shibuya H, Niimoto T, Kongcharoensombat W and Ochi M: Silencing microRNA-34a inhibits chondrocyte apoptosis in a rat osteoarthritis model in vitro. Rheumatology (Oxford). 49:2054–2060. 2010. View Article : Google Scholar

9 

Miyaki S, Nakasa T, Otsuki S, Grogan SP, Higashiyama R, Inoue A, Kato Y, Sato T, Lotz MK and Asahara H: MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates interleukin-1 responses. Arthritis Rheum. 60:2723–2730. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Smyth GK: Limma: Linear models for microarray data. Bioinformatics and computational biology solutions using R and Bioconductor. Springer; pp. 397–420. 2005, View Article : Google Scholar

11 

Benjamini Y and Hochberg Y: Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Stat Soc Series B Stat Methodol. 289–300. 1995.

12 

Nam S, Li M, Choi K, Balch C, Kim S and Nephew KP: MicroRNA and mRNA integrated analysis (MMIA): A web tool for examining biological functions of microRNA expression. Nucleic Acids Res. 37:W356–W362. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Szklarczyk D, Franceschini A, Kuhn M, Simonovic M, Roth A, Minguez P, Doerks T, Stark M, Muller J, Bork P, et al: The STRING database in 2011: Functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 39:D561–D568. 2011. View Article : Google Scholar :

14 

Smoot ME, Ono K, Ruscheinski J, Wang PL and Ideker T: Cytoscape 2.8: New features for data integration and network visualization. Bioinformatics. 27:431–432. 2011. View Article : Google Scholar :

15 

Maere S, Heymans K and Kuiper M: BiNGO: A Cytoscape plugin to assess overrepresentation of gene ontology categories in biological networks. Bioinformatics. 21:3448–3449. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Zhang B, Kirov S and Snoddy J: WebGestalt: An integrated system for exploring gene sets in various biological contexts. Nucleic Acids Res. 33:W741–W748. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Duncan D, Prodduturi N and Zhang B: WebGestalt2: An updated and expanded version of the web-based gene set analysis toolkit. BMC Bioinformatics. 11:P102010. View Article : Google Scholar

18 

Pattappa G, Li Z, Peroglio M, Wismer N, Alini M and Grad S: Diversity of intervertebral disc cells: Phenotype and function. J Anat. 221:480–496. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Le Maitre CL, Pockert A, Buttle DJ, Freemont AJ and Hoyland JA: Matrix synthesis and degradation in human intervertebral disc degeneration. Biochem Soc Trans. 35:652–655. 2007. View Article : Google Scholar : PubMed/NCBI

20 

de Oliveira CP, Rodrigues LM, Fregni MV, Gotfryd A, Made AM and Pinhal MA: Extracellular matrix remodeling in experimental intervertebral disc degeneration. Acta Ortop Bras. 21:144–149. 2013. View Article : Google Scholar

21 

Medina R, Zaidi SK, Liu CG, Stein JL, van Wijnen AJ, Croce CM and Stein GS: MicroRNAs 221 and 222 bypass quiescence and compromise cell survival. Cancer Res. 68:2773–2780. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Koff A: How to decrease p27Kip1 levels during tumor development. Cancer Cell. 9:75–76. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Wurz K, Garcia RL, Goff BA, Mitchell PS, Lee JH, Tewari M and Swisher EM: MiR-221 and MiR-222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B, CDKNIC and overall survival. Genes Chromosomes Cancer. 49:577–584. 2010.PubMed/NCBI

24 

Zhang K, Zhang H, Zhou X, Tang WB, Xiao L, Liu YH, Liu H, Peng YM, Sun L and Liu FY: MiRNA589 regulates epithelial-mesenchymal transition in human peritoneal mesothelial cells. J Biomed Biotechnol. 2012:6730962012. View Article : Google Scholar : PubMed/NCBI

25 

Risbud MV and Shapiro IM: Role of cytokines in intervertebral disc degeneration: Pain and disc content. Nat Rev Rheumatol. 10:44–56. 2014. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Hu P, Feng B, Wang G, Ning B and Jia T: Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Mol Med Rep 12: 4925-4930, 2015.
APA
Hu, P., Feng, B., Wang, G., Ning, B., & Jia, T. (2015). Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Molecular Medicine Reports, 12, 4925-4930. https://doi.org/10.3892/mmr.2015.4022
MLA
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12.4 (2015): 4925-4930.
Chicago
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12, no. 4 (2015): 4925-4930. https://doi.org/10.3892/mmr.2015.4022
Copy and paste a formatted citation
x
Spandidos Publications style
Hu P, Feng B, Wang G, Ning B and Jia T: Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Mol Med Rep 12: 4925-4930, 2015.
APA
Hu, P., Feng, B., Wang, G., Ning, B., & Jia, T. (2015). Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration. Molecular Medicine Reports, 12, 4925-4930. https://doi.org/10.3892/mmr.2015.4022
MLA
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12.4 (2015): 4925-4930.
Chicago
Hu, P., Feng, B., Wang, G., Ning, B., Jia, T."Microarray based analysis of gene regulation by microRNA in intervertebral disc degeneration". Molecular Medicine Reports 12, no. 4 (2015): 4925-4930. https://doi.org/10.3892/mmr.2015.4022
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