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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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October-2015 Volume 12 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity

  • Authors:
    • Chao Yu
    • Min Wang
    • Meiyuan Chen
    • Yang Huang
    • Jianxin Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, P.R. China, Department of Biliary‑Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China
  • Pages: 5135-5140
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/mmr.2015.4031
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Abstract

The present study investigated the role of microRNA (miR)‑138‑5p in regulating carcinoma migration and sensitivity to chemotherapy in pancreatic cancer. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to assess the expression levels of miR‑138‑5p in pancreatic cancer cell lines and primary carcinoma tissues from human patients. A lentiviral vector, containing miR‑138‑5p mimics (lv‑miR‑138‑m) or miR‑138‑5p inhibitor (lv‑miR‑138‑i), was used to either upregulate or downregulate the expression levels of miR‑138‑5p in PANC‑1 cells, respectively. The effects of miR‑138‑3p regulation on pancreatic cancer cell migration and sensitivity to chemotherapy were examined. The predicted targeting of miR‑138‑5p on vimentin (VIM) was assessed by western blotting in PANC‑1 cells. VIM was subsequently downregulated using small interfering (si)RNA to determine its effect on miR‑138‑5p‑modulated pancreatic cancer cell development. The expression levels of miR‑138‑5p were downregulated in pancreatic cancer cell lines and primary carcinoma tissues. In PANC‑1 cells, lentivirus-mediated upregulation of miR‑138‑5p inhibited cancer cell migration and increased cell chemosensitivity to 5‑fluorouracil (5‑FU). By contrast, downregulation of miR‑138‑5p promoted cancer cell migration and decreased cell chemosensitivity to 5‑FU. A luciferase assay revealed that VIM was a direct target of miR‑138‑5p. Western blotting demonstrated that VIM was downregulated upon the upregulation of miR‑138‑5p in PANC‑1 cells. siRNA‑mediated downregulation of VIM inhibited pancreatic cancer cell migration in the control and miR‑138‑5p downregulated PANC‑1 cells. The present study demonstrated that miR‑138‑5p is important in regulating pancreatic cancer development, possibly through targeting VIM.
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View References

1 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Zhang S, et al: Report of incidence and mortality in China cancer registries, 2009. Chin J Cancer Res. 25:10–21. 2013.PubMed/NCBI

3 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Hirata K, Egawa S, Kimura Y, et al: Current status of surgery for pancreatic cancer. Dig Surg. 24:137–147. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Gillen S, Schuster T, Meyer Zum Büschenfelde C, Friess H and Kleeff J: Preoperative/neoadjuvant therapy in pancreatic cancer: a systematic review and meta-analysis of response and resection percentages. PLoS Med. 7:e10002672010. View Article : Google Scholar : PubMed/NCBI

6 

Pillai RS: MicroRNA function: Multiple mechanisms for a tiny RNA? RNA. 11:1753–1761. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Feng W and Feng Y: MicroRNAs in neural cell development and brain diseases. Sci China Life Sci. 54:1103–1112. 2011. View Article : Google Scholar

9 

Bian S and Sun T: Functions of noncoding RNAs in neural development and neurological diseases. Mol Neurobiol. 44:359–373. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Papagiannakopoulos T and Kosik KS: MicroRNAs: Regulators of oncogenesis and stemness. BMC Med. 6:152008. View Article : Google Scholar : PubMed/NCBI

11 

Blandino G, Fazi F, Donzelli S, et al: Tumor suppressor microRNAs: A novel non-coding alliance against cancer. FEBS Lett. 588:2639–2652. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Palanichamy JK and Rao DS: miRNA dysregulation in cancer: Towards a mechanistic understanding. Front Genet. 5:542014. View Article : Google Scholar : PubMed/NCBI

13 

Wang Y, Kim S and Kim IM: Regulation of metastasis by microRNAs in ovarian cancer. Front Oncol. 4:1432014. View Article : Google Scholar : PubMed/NCBI

14 

Othman N and Nagoor NH: The role of microRNAs in the regulation of apoptosis in lung cancer and its application in cancer treatment. Biomed Res Int. 2014:3180302014. View Article : Google Scholar : PubMed/NCBI

15 

Han C, Yu Z, Duan Z and Kan Q: Role of microRNA-1 in human cancer and its therapeutic potentials. Biomed Res Int. 2014:4283712014. View Article : Google Scholar : PubMed/NCBI

16 

Gao Y, Fan X, Li W, Ping W, Deng Y and Fu X: miR-138-5p reverses gefitinib resistance in non-small cell lung cancer cells via negatively regulating G protein-coupled receptor 124. Biochem Biophys Res Commun. 446:179–186. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang W, Zhao LJ, Tan YX, Ren H and Qi ZT: MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma. Carcinogenesis. 33:1113–1120. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Zhao X, Yang L, Hu J and Ruan J: miR-138 might reverse multidrug resistance of leukemia cells. Leuk Res. 34:1078–1082. 2010. View Article : Google Scholar

19 

Radulovich N, Qian JY and Tsao MS: Human pancreatic duct epithelial cell model for KRAS transformation. Methods Enzymol. 439:1–13. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Zhang L, Mizumoto K, Sato N, et al: Quantitative determination of apoptotic death in cultured human pancreatic cancer cells by propidium iodide and digitonin. Cancer Lett. 142:129–137. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Singh S, Sadacharan S, Su S, Belldegrun A, Persad S and Singh G: Overexpression of vimentin: Role in the invasive phenotype in an androgen-independent model of prostate cancer. Cancer Res. 63:2306–2311. 2003.PubMed/NCBI

22 

Wang L, Gu F, Liu CY, Wang RJ, Li J and Xu JY: High level of FOXC1 expression is associated with poor prognosis in pancreatic ductal adenocarcinoma. Tumour Biol. 34:853–858. 2013. View Article : Google Scholar

23 

Li ZM, Wen YJ, Yang HB, et al: Enhanced expression of human vimentin intermediate filaments in hepatocellular carcinoma cells decreases their proliferative and invasive abilities in vitro. Zhonghua Zhong Liu Za Zhi. 30:408–412. 2008.In Chinese. PubMed/NCBI

24 

Gilles C, Polette M, Mestdagt M, et al: Transactivation of vimentin by beta-catenin in human breast cancer cells. Cancer Res. 63:2658–2664. 2003.PubMed/NCBI

25 

Korsching E, Packeisen J, Liedtke C, et al: The origin of vimentin expression in invasive breast cancer: Epithelial-mesenchymal transition, myoepithelial histogenesis or histogenesis from progenitor cells with bilinear differentiation potential? J Oathol. 206:451–457. 2005. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Yu C, Wang M, Chen M, Huang Y and Jiang J: Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity. Mol Med Rep 12: 5135-5140, 2015.
APA
Yu, C., Wang, M., Chen, M., Huang, Y., & Jiang, J. (2015). Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity. Molecular Medicine Reports, 12, 5135-5140. https://doi.org/10.3892/mmr.2015.4031
MLA
Yu, C., Wang, M., Chen, M., Huang, Y., Jiang, J."Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity". Molecular Medicine Reports 12.4 (2015): 5135-5140.
Chicago
Yu, C., Wang, M., Chen, M., Huang, Y., Jiang, J."Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity". Molecular Medicine Reports 12, no. 4 (2015): 5135-5140. https://doi.org/10.3892/mmr.2015.4031
Copy and paste a formatted citation
x
Spandidos Publications style
Yu C, Wang M, Chen M, Huang Y and Jiang J: Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity. Mol Med Rep 12: 5135-5140, 2015.
APA
Yu, C., Wang, M., Chen, M., Huang, Y., & Jiang, J. (2015). Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity. Molecular Medicine Reports, 12, 5135-5140. https://doi.org/10.3892/mmr.2015.4031
MLA
Yu, C., Wang, M., Chen, M., Huang, Y., Jiang, J."Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity". Molecular Medicine Reports 12.4 (2015): 5135-5140.
Chicago
Yu, C., Wang, M., Chen, M., Huang, Y., Jiang, J."Upregulation of microRNA‑138‑5p inhibits pancreatic cancer cell migration and increases chemotherapy sensitivity". Molecular Medicine Reports 12, no. 4 (2015): 5135-5140. https://doi.org/10.3892/mmr.2015.4031
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