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Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells

Retraction in: /10.3892/mmr.2021.12079
  • Authors:
    • Yue‑Yong Zhu
    • Hong‑Yan Huang
    • Yin‑Lian Wu
  • View Affiliations / Copyright

    Affiliations: Liver Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Gastroenterology, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5012-5018
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/mmr.2015.4033
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Abstract

Hepatocellular carcinoma (HCC) is an aggressive form of cancer, with high rates of morbidity and mortality, a poor prognosis and limited therapeutic options. The objective of the present study was to demonstrate the anticancer activity of oleanolic acid in HepG2 human HCC cells. Cell viability was evaluated using an MTT assay, following administration of various doses of oleanolic acid. The effect of oleanolic acid on cell cycle phase distribution and mitochondrial membrane potential was evaluated using flow cytometry with propidium iodide and rhodamine‑123 DNA‑binding cationic fluorescent dyes. Fluorescence microscopy was employed to detect morphological changes in HepG2 cells following oleanolic acid treatment. The results revealed that oleanolic acid induced a dose‑dependent, as well as time‑dependent inhibition in the growth of HepG2 cancer cells. Following acridine orange and ethidium bromide staining, treatment with various doses (0, 5, 25 and 50 µM) of oleanolic acid induced typical morphological changes associated with apoptosis, including cell shrinkage, membrane blebbing, nuclear condensation and apoptotic body formation. Cell cycle analysis revealed that oleanolic acid induced cell cycle arrest in HepG2 cells at the sub‑G1 (apoptotic) phase of the cell cycle, in a dose‑dependent manner. Staining with Annexin V‑fluorescein isothiocyanate and propidium iodide revealed that apoptosis occurred early in these cells. Oleanolic acid treatment also resulted in fragmentation of nuclear DNA in a dose‑dependent manner, producing the typical features of DNA laddering on an agarose gel. The results also demonstrated that oleanolic acid treatment resulted in a potent loss of mitochondrial membrane potential, which also occurred in a dose‑dependent manner. Therefore, oleanolic acid may be used as a therapeutic agent in the treatment of human HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu YY, Huang HY and Wu YL: Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079. Mol Med Rep 12: 5012-5018, 2015.
APA
Zhu, Y., Huang, H., & Wu, Y. (2015). Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079. Molecular Medicine Reports, 12, 5012-5018. https://doi.org/10.3892/mmr.2015.4033
MLA
Zhu, Y., Huang, H., Wu, Y."Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079". Molecular Medicine Reports 12.4 (2015): 5012-5018.
Chicago
Zhu, Y., Huang, H., Wu, Y."Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079". Molecular Medicine Reports 12, no. 4 (2015): 5012-5018. https://doi.org/10.3892/mmr.2015.4033
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu YY, Huang HY and Wu YL: Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079. Mol Med Rep 12: 5012-5018, 2015.
APA
Zhu, Y., Huang, H., & Wu, Y. (2015). Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079. Molecular Medicine Reports, 12, 5012-5018. https://doi.org/10.3892/mmr.2015.4033
MLA
Zhu, Y., Huang, H., Wu, Y."Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079". Molecular Medicine Reports 12.4 (2015): 5012-5018.
Chicago
Zhu, Y., Huang, H., Wu, Y."Anticancer and apoptotic activities of oleanolic acid are mediated through cell cycle arrest and disruption of mitochondrial membrane potential in HepG2 human hepatocellular carcinoma cells Retraction in /10.3892/mmr.2021.12079". Molecular Medicine Reports 12, no. 4 (2015): 5012-5018. https://doi.org/10.3892/mmr.2015.4033
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