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Article

Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway

  • Authors:
    • Lichao Wang
    • Buling Wu
    • Yu Zhang
    • Zhihui Tian
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 5267-5273
    |
    Published online on: July 3, 2015
       https://doi.org/10.3892/mmr.2015.4034
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Abstract

Investigations into the role of hypoxia have concentrated on hypoxic damage to cells and the associated adaptative mechanisms, however, the effects of hypoxia on cell proliferation and differentiation have received less attention. The present study aimed to investigate the role of hypoxia on the proliferation of MC3T3‑E1 cells and examine the molecular mechanism involved. Cells treated with low levels of hypoxia had an increased percentage of S phase cells and a decreased percentage of G1 phase cells, promoted the level of cell proliferation‑associated proteins, proliferating cell nuclear antigen and cyclin D. In addition, hypoxia increased cell proliferation by upregulating the expression of hypoxia‑inducible factor (HIF)‑1α. The phosphoinositide 3‑kinase/Akt and mitogen‑activated protein kinase/extracellular signal‑regulated kinase pathways augmented the expression of HIF‑1α in the MC3T3‑E1 cells. The present study demonstrated that hypoxia induces positive effects on osteoblast proliferation, suggesting a novel strategy in the treatment of osteoporosis.
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1 

Hadji P, Klein S, Gothe H, Häussler B, Kless T, Schmidt T, Steinle T, Verheyen F and Linder R: The epidemiology of osteoporosis - Bone Evaluation Study (BEST): an analysis of routine health insurance data. Dtsch Arztebl Int. 110:52–57. 2013.PubMed/NCBI

2 

Kanis JA, McCloskey EV, Johansson H, Oden A, Melton LJ 3rd and Khaltaev N: A reference standard for the description of osteoporosis. Bone. 42:467–475. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Riggs BL and Melton LJ 3rd: The worldwide problem of osteoporosis: Insights afforded by epidemiology. Bone. 17:505S–511S. 1995. View Article : Google Scholar : PubMed/NCBI

4 

Soleymanian A, Niknami S, Hajizadeh E, Shojaeizadeh D and Montazeri A: Development and validation of a health belief model based instrument for measuring factors influencing exercise behaviors to prevent osteoporosis in pre-menopausal women (HOPE). BMC Musculoskelet Disord. 15:612014. View Article : Google Scholar : PubMed/NCBI

5 

Muz B, Khan MN, Kiriakidis S and Paleolog EM: Hypoxia. The role of hypoxia and HIF-dependent signalling events in rheumatoid arthritis. Arthritis Res Ther. 11:2012009. View Article : Google Scholar : PubMed/NCBI

6 

Scherbakov AM, Stefanova LB, Yakushina IA and Krasilnikov MA: Beta-catenin signaling pathway and the tolerance of breast cancer cells to hypoxic conditions. Klin Lab Diagn. 10:68–70. 2013.

7 

Liu SS, Wang HY, Tang JM and Zhou XM: Hypoxia-induced collagen synthesis of human lung fibroblasts by activating the angiotensin system. Int J Mol Sci. 14:24029–24045. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Banasiak KJ and Haddad GG: Hypoxia-induced apoptosis: effect of hypoxic severity and role of p53 in neuronal cell death. Brain Res. 797:295–304. 1998. View Article : Google Scholar : PubMed/NCBI

9 

Calzado MA, de la Vega L, Möller A, Bowtell DD and Schmitz ML: An inducible autoregulatory loop between HIPK2 and Siah2 at the apex of the hypoxic response. Nat Cell Biol. 11:85–91. 2009. View Article : Google Scholar

10 

Yang XM, Wang YS, Zhang J, Li Y, Xu JF, Zhu J, Zhao W, Chu DK and Wiedemann P: Role of PI3K/Akt and MEK/ERK in mediating hypoxia-induced expression of HIF-1alpha and VEGF in laser-induced rat choroidal neovascularization. Invest Ophthalmol Vis Sci. 50:1873–1879. 2009. View Article : Google Scholar

11 

Moon EJ, Sonveaux P, Porporato PE, Danhier P, Gallez B, Batinic-Haberle I, Nien YC, Schroeder T and Dewhirst MW: NADPH oxidase-mediated reactive oxygen species production activates hypoxia-inducible factor-1 (HIF-1) via the ERK pathway after hyperthermia treatment. Proc Natl Acad Sci USA. 107:20477–20482. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Fujita T, Azuma Y, Fukuyama R, Hattori Y, Yoshida C, Koida M, Ogita K and Komori T: Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling. J Cell Biol. 166:85–95. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Zhang LY, Zhou YY, Chen F, Wang B, Li J, Deng YW, Liu WD, Wang ZG, Li YW, Li DZ, Lv GH and Yin BL: Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway. Braz J Med Biol Res. 44:618–623. 2011.PubMed/NCBI

14 

He X, Brenchley PE, Jayson GC, Hampson L, Davies J and Hampson IN: Hypoxia increases heparanase-dependent tumor cell invasion, which can be inhibited by antiheparanase antibodies. Cancer Res. 64:3928–3933. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Shao Y and Zhao FQ: Emerging evidence of the physiological role of hypoxia in mammary development and lactation. J Anim Sci Biotechnol. 5:92014. View Article : Google Scholar : PubMed/NCBI

16 

Ding X, Lu C, Liu H, Rao S, Cai J, Liu S, Kriegel AJ, Greene AS, Liang M and Ding X: Hypoxic preconditioning with cobalt of bone marrow mesenchymal stem cells improves cell migration and enhances therapy for treatment of ischemic acute kidney injury. PLoS One. 8:e627032013. View Article : Google Scholar : PubMed/NCBI

17 

Hu S, Yan G, Xu H, He W, Liu Z and Ma G: Hypoxic preconditioning increases survival of cardiac progenitor cells via the Pim-1 kinase-mediated anti-apoptotic effect. Circ J. 78:724–731. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Semenza GL: Hypoxia-inducible factors in physiology and medicine. Cell. 148:399–408. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Liu Y, Ma Z, Zhao C, Wang Y, Wu G, Xiao J, McClain CJ, Li X and Feng W: HIF-1α and HIF-2α are critically involved in hypoxia-induced lipid accumulation in hepatocytes through reducing PGC-1α-mediated fatty acid β-oxidation. Toxicol Lett. 226:117–123. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Tsai YP and Wu KJ: Hypoxia-regulated target genes implicated in tumor metastasis. J Biomed Sci. 19:1022012. View Article : Google Scholar : PubMed/NCBI

21 

Al-Motabagani MA: Histological changes in the alveolar structure of the rat lung after exposure to hyperoxia. Ital J Anat Embryol. 110:209–223. 2005.

22 

Wang X, Wang Y, Kim HP, Nakahira K, Ryter SW and Choi AM: Carbon monoxide protects against hyperoxia-induced endothelial cell apoptosis by inhibiting reactive oxygen species formation. J Biol Chem. 282:1718–1726. 2007. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Wu B, Zhang Y and Tian Z: Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway. Mol Med Rep 12: 5267-5273, 2015.
APA
Wang, L., Wu, B., Zhang, Y., & Tian, Z. (2015). Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway. Molecular Medicine Reports, 12, 5267-5273. https://doi.org/10.3892/mmr.2015.4034
MLA
Wang, L., Wu, B., Zhang, Y., Tian, Z."Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway". Molecular Medicine Reports 12.4 (2015): 5267-5273.
Chicago
Wang, L., Wu, B., Zhang, Y., Tian, Z."Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway". Molecular Medicine Reports 12, no. 4 (2015): 5267-5273. https://doi.org/10.3892/mmr.2015.4034
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Wu B, Zhang Y and Tian Z: Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway. Mol Med Rep 12: 5267-5273, 2015.
APA
Wang, L., Wu, B., Zhang, Y., & Tian, Z. (2015). Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway. Molecular Medicine Reports, 12, 5267-5273. https://doi.org/10.3892/mmr.2015.4034
MLA
Wang, L., Wu, B., Zhang, Y., Tian, Z."Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway". Molecular Medicine Reports 12.4 (2015): 5267-5273.
Chicago
Wang, L., Wu, B., Zhang, Y., Tian, Z."Hypoxia promotes the proliferation of MC3T3-E1 cells via the hypoxia-inducible factor-1α signaling pathway". Molecular Medicine Reports 12, no. 4 (2015): 5267-5273. https://doi.org/10.3892/mmr.2015.4034
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