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Article

Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6

  • Authors:
    • Xi Chen
    • Mingxuan Xie
    • Da Liu
    • Ke Shi
  • View Affiliations / Copyright

    Affiliations: Department of Geriatric Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 5155-5162
    |
    Published online on: July 3, 2015
       https://doi.org/10.3892/mmr.2015.4036
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Abstract

Premature ovarian failure (POF), an ovarian disorder of multifactorial origin, is defined as the occurrence of amenorrhea, hypergonadotropism and hypoestrogenism in females <40 years old. Apoptosis of ovarian granulosa cells is important in POF and understanding the regulatory mechanism underlying ovarian granulosa cell apoptosis may be beneficial for the management of POF. Increasing evidence suggests that microRNAs (miRs) have a regulatory function in oocyte maturation and ovarian follicular development. In the present study, the expression of miR‑146a in plasma and ovarian granulosa cells obtained from patients with POF, its effect on the apoptosis of ovarian granulosa cells and the possible underlying mechanisms were examined. The present study demonstrated that compared with the control groups, the expression of miR‑146a in the plasma and in ovarian granulosa cells of patients with POF was significantly upregulated. Furthermore, it was found that miR‑146a simultaneously targeted interleukin‑1 receptor‑associated kinase (IRAK1) and tumor necrosis factor receptor‑associated factor 6 (TRAF6), which regulated the activity of nuclear factor‑κB and IκBα. In addition, the results demonstrated that inhibition of the caspase cascade by caspase inhibitors attenuated the effects of miR‑146a on ovarian granulosa cell apoptosis. Taken together, these results suggest that miR‑146a has an important promoting effect on the apoptosis of granulosa cells by targeting IRAK1 and TRAF6 via the caspase cascade pathway. These results may be useful for the management of POF.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Xie M, Liu D and Shi K: Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6. Mol Med Rep 12: 5155-5162, 2015.
APA
Chen, X., Xie, M., Liu, D., & Shi, K. (2015). Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6. Molecular Medicine Reports, 12, 5155-5162. https://doi.org/10.3892/mmr.2015.4036
MLA
Chen, X., Xie, M., Liu, D., Shi, K."Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6". Molecular Medicine Reports 12.4 (2015): 5155-5162.
Chicago
Chen, X., Xie, M., Liu, D., Shi, K."Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6". Molecular Medicine Reports 12, no. 4 (2015): 5155-5162. https://doi.org/10.3892/mmr.2015.4036
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Xie M, Liu D and Shi K: Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6. Mol Med Rep 12: 5155-5162, 2015.
APA
Chen, X., Xie, M., Liu, D., & Shi, K. (2015). Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6. Molecular Medicine Reports, 12, 5155-5162. https://doi.org/10.3892/mmr.2015.4036
MLA
Chen, X., Xie, M., Liu, D., Shi, K."Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6". Molecular Medicine Reports 12.4 (2015): 5155-5162.
Chicago
Chen, X., Xie, M., Liu, D., Shi, K."Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6". Molecular Medicine Reports 12, no. 4 (2015): 5155-5162. https://doi.org/10.3892/mmr.2015.4036
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