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Article Open Access

8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells

  • Authors:
    • Qing Ding
    • Yi Chen
    • Qing Zhang
    • Yanling Guo
    • Zhi Huang
    • Liqing Dai
    • Sudan Cao
  • View Affiliations / Copyright

    Affiliations: Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, P.R.China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5100-5108
    |
    Published online on: July 3, 2015
       https://doi.org/10.3892/mmr.2015.4039
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Abstract

8‑bromo‑7‑methoxychrysin (BrMC), a novel chrysin analog, was reported to have anti‑cancer activities. The aim of the present study was to investigate the molecular mechanism of 8‑bromo‑7‑methoxychrysin (BrMC)‑induced apoptosis via the Akt/forkhead box O3a (FOXO3a) pathway in cisplatin (DDP)‑sensitive and ‑resistant ovarian cancer cells. The human ovarian cancer cell lines A2780 and A2780/DDP were cultured in vitro. Various molecular techniques were used to assess the expression of FOXO3a and B cell lymphoma 2 (Bcl‑2)‑interacting mediator of cell death (Bim) in cisplatin‑sensitive and ‑resistant ovarian cancer cells. Different concentrations of BrMC induced apoptosis in cisplatin‑sensitive and ‑resistant ovarian cancer cells. BrMC‑induced apoptotic cell death occurred mainly by the activation of Akt, which was accompanied by the overexpression of transcription factor FOXO3a, with a concomitant increase in the expression levels of Bim. Silencing Bim expression by using small interfering RNA, attenuated the induction of apoptosis by BrMC treatment. The results indicated that BrMC‑induced apoptosis in cisplatin‑sensitive and ‑resistant ovarian cancer cells may occur via the regulation of Akt/FOXO3a, leading to Bim transcription.
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Copy and paste a formatted citation
Spandidos Publications style
Ding Q, Chen Y, Zhang Q, Guo Y, Huang Z, Dai L and Cao S: 8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells. Mol Med Rep 12: 5100-5108, 2015.
APA
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., & Cao, S. (2015). 8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells. Molecular Medicine Reports, 12, 5100-5108. https://doi.org/10.3892/mmr.2015.4039
MLA
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., Cao, S."8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells". Molecular Medicine Reports 12.4 (2015): 5100-5108.
Chicago
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., Cao, S."8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells". Molecular Medicine Reports 12, no. 4 (2015): 5100-5108. https://doi.org/10.3892/mmr.2015.4039
Copy and paste a formatted citation
x
Spandidos Publications style
Ding Q, Chen Y, Zhang Q, Guo Y, Huang Z, Dai L and Cao S: 8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells. Mol Med Rep 12: 5100-5108, 2015.
APA
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., & Cao, S. (2015). 8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells. Molecular Medicine Reports, 12, 5100-5108. https://doi.org/10.3892/mmr.2015.4039
MLA
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., Cao, S."8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells". Molecular Medicine Reports 12.4 (2015): 5100-5108.
Chicago
Ding, Q., Chen, Y., Zhang, Q., Guo, Y., Huang, Z., Dai, L., Cao, S."8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells". Molecular Medicine Reports 12, no. 4 (2015): 5100-5108. https://doi.org/10.3892/mmr.2015.4039
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