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October-2015 Volume 12 Issue 4

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Article Open Access

Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway

  • Authors:
    • Weimin Ni
    • Yan Fang
    • Lei Tong
    • Zhaoxue Tong
    • Fuxin Yi
    • Jianwu Qiu
    • Rui Wang
    • Xiaojie Tong
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Human Anatomy, Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China
    Copyright: © Ni et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5086-5092
    |
    Published online on: July 7, 2015
       https://doi.org/10.3892/mmr.2015.4049
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Abstract

Girdin, an actin‑binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound‑healing assay, transwell invasion assay, reverse transcription‑quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K‑Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Ni W, Fang Y, Tong L, Tong Z, Yi F, Qiu J, Wang R and Tong X: Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway. Mol Med Rep 12: 5086-5092, 2015.
APA
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J. ... Tong, X. (2015). Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway. Molecular Medicine Reports, 12, 5086-5092. https://doi.org/10.3892/mmr.2015.4049
MLA
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J., Wang, R., Tong, X."Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway". Molecular Medicine Reports 12.4 (2015): 5086-5092.
Chicago
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J., Wang, R., Tong, X."Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway". Molecular Medicine Reports 12, no. 4 (2015): 5086-5092. https://doi.org/10.3892/mmr.2015.4049
Copy and paste a formatted citation
x
Spandidos Publications style
Ni W, Fang Y, Tong L, Tong Z, Yi F, Qiu J, Wang R and Tong X: Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway. Mol Med Rep 12: 5086-5092, 2015.
APA
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J. ... Tong, X. (2015). Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway. Molecular Medicine Reports, 12, 5086-5092. https://doi.org/10.3892/mmr.2015.4049
MLA
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J., Wang, R., Tong, X."Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway". Molecular Medicine Reports 12.4 (2015): 5086-5092.
Chicago
Ni, W., Fang, Y., Tong, L., Tong, Z., Yi, F., Qiu, J., Wang, R., Tong, X."Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway". Molecular Medicine Reports 12, no. 4 (2015): 5086-5092. https://doi.org/10.3892/mmr.2015.4049
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