Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway

Ni,Weimin; Fang,Yan; Tong,Lei; Tong,Zhaoxue; Yi,Fuxin; Qiu,Jianwu; Wang,Rui; Tong,Xiaojie

doi:10.3892/mmr.2015.4049

Girdin regulates the migration and invasion of glioma cells via the PI3K‑Akt signaling pathway

Authors:
- Weimin Ni
- Yan Fang
- Lei Tong
- Zhaoxue Tong
- Fuxin Yi
- Jianwu Qiu
- Rui Wang
- Xiaojie Tong
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Affiliations: Department of Human Anatomy, Histology and Embryology, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Human Anatomy, Histology and Embryology, Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, P.R. China
Published online on: July 7, 2015 https://doi.org/10.3892/mmr.2015.4049
Pages: 5086-5092
Copyright: © Ni et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Girdin, an actin‑binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short‑hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound‑healing assay, transwell invasion assay, reverse transcription‑quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)‑2 and MMP‑9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K‑Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.

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