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Article

Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways

  • Authors:
    • Yanhui Zhu
    • Hairong Tao
    • Chen Jin
    • Yonzhang Liu
    • Xiongwei Lu
    • Xiaopeng Hu
    • Xiang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201900, P.R. China
  • Pages: 5573-5579
    |
    Published online on: July 9, 2015
       https://doi.org/10.3892/mmr.2015.4068
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Abstract

Transforming growth factor (TGF)‑β regulates the anabolic metabolism of articular cartilage and prevents cartilage degradation. TGF‑β1 influences cellular proliferation, differentiation and the extracellular matrix through activation of the extracellular signal‑regulated kinase (ERK)1/2 and Smad2/3 signaling pathways. However, it has remained to be fully elucidated precisely how the ERK1/2 and Smad2/3 signaling pathways mediate anabolic processes of articular cartilage. The present study investigated how ERK1/2 and Smad2/3 signaling mediate TGF‑β1‑stimulated type II collagen and aggrecan expression in rat chondrocytes. The results confirmed that TGF‑β1 stimulates type II collagen and aggrecan expression in rat chondrocytes, and furthermore, that the ERK1/2 and Smad2/3 signaling pathways were activated by TGF‑β1. Conversely, the TGF‑β receptor I (ALK5) kinase inhibitor SB525334 significantly impaired TGF‑β1‑induced type II collagen and aggrecan expression, coinciding with a reduction of ERK1/2 and Smad3 phosphorylation. In addition, TGF‑β1‑induced type II collagen and aggrecan expression were significantly suppressed by ERK1/2 inhibitor PD98059. Similarly, TGF‑β1‑stimulated type II collagen and aggrecan expression were decreased in the presence of a Smad3 phosphorylation inhibitor SIS3. Therefore, the present study demonstrated that the ERK1/2 and Smad2/3 signaling pathways regulate type II collagen and aggrecan expression in rat chondrocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu Y, Tao H, Jin C, Liu Y, Lu X, Hu X and Wang X: Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways. Mol Med Rep 12: 5573-5579, 2015.
APA
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., & Wang, X. (2015). Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways. Molecular Medicine Reports, 12, 5573-5579. https://doi.org/10.3892/mmr.2015.4068
MLA
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., Wang, X."Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways". Molecular Medicine Reports 12.4 (2015): 5573-5579.
Chicago
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., Wang, X."Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways". Molecular Medicine Reports 12, no. 4 (2015): 5573-5579. https://doi.org/10.3892/mmr.2015.4068
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu Y, Tao H, Jin C, Liu Y, Lu X, Hu X and Wang X: Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways. Mol Med Rep 12: 5573-5579, 2015.
APA
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., & Wang, X. (2015). Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways. Molecular Medicine Reports, 12, 5573-5579. https://doi.org/10.3892/mmr.2015.4068
MLA
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., Wang, X."Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways". Molecular Medicine Reports 12.4 (2015): 5573-5579.
Chicago
Zhu, Y., Tao, H., Jin, C., Liu, Y., Lu, X., Hu, X., Wang, X."Transforming growth factor‑β1 induces type II collagen and aggrecan expression via activation of extracellular signal‑regulated kinase 1/2 and Smad2/3 signaling pathways". Molecular Medicine Reports 12, no. 4 (2015): 5573-5579. https://doi.org/10.3892/mmr.2015.4068
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