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Article

Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts

  • Authors:
    • Xiu‑Mei Li
    • Xue‑Gang Luo
    • Kun Li
    • Nan Wang
    • Er‑Bin Hua
    • Yao Zhang
    • Tong‑Cun Zhang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China
  • Pages: 5423-5428
    |
    Published online on: July 15, 2015
       https://doi.org/10.3892/mmr.2015.4080
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Abstract

According to previous studies, hyperoside possesses myocardial protective effects. To investigate whether isoquercitrin and isohyperoside have similar functions, the protective effects of isoquercitrin and isohyperoside against H2O2‑induced injury in H9c2 rat cardiomyoblasts were evaluated using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. The mechanism of action was investigated by assessing the leakage of lactate dehydrogenase (LDH) of hyperoside and isoquercitrin‑pretreated H9c2 cardiomyocytes following H2O2‑induced injury, and examining their effects on the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) activity. The isolation of two flavonoid glycosides from H. ascyron was performed, following extraction, using semi‑preparative high performance liquid chromatography. Using the spectral characteristics, the structures of these compounds were identified as isoquercitrin and isohyperoside. This was the first time, to the best of our knowledge, that isohyperoside has been identified from H. ascyron. The results revealed that isoquercitrin and isohyperoside possessed similar protective effects to hyperoside against H2O2‑induced injury in H9c2 cells. The half maximal inhibitory concentration values of hyperoside, isoquercitrin and isohyperoside were 0.0008, 0.0017 and 0.0002 µM, respectively. Based on these results, isohyperoside possessed more marked protective effects against H2O2‑induced injury in the H9c2 cardiomyoblasts. The significant reduction in LDH leakage, decrease in MDA level and increase in SOD activity also provided evidence of the cardioprotective effects of isoquercitrin and isohyperoside. The present study reported for the first time, to the best of our knowledge, the myocardial protective effects of isoquercitrin and isohyperoside. The mechanism of action may involve protection of the cell membrane from oxidative damage.
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Copy and paste a formatted citation
Spandidos Publications style
Li XM, Luo XG, Li K, Wang N, Hua EB, Zhang Y and Zhang TC: Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts. Mol Med Rep 12: 5423-5428, 2015.
APA
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., & Zhang, T. (2015). Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts. Molecular Medicine Reports, 12, 5423-5428. https://doi.org/10.3892/mmr.2015.4080
MLA
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., Zhang, T."Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts". Molecular Medicine Reports 12.4 (2015): 5423-5428.
Chicago
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., Zhang, T."Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts". Molecular Medicine Reports 12, no. 4 (2015): 5423-5428. https://doi.org/10.3892/mmr.2015.4080
Copy and paste a formatted citation
x
Spandidos Publications style
Li XM, Luo XG, Li K, Wang N, Hua EB, Zhang Y and Zhang TC: Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts. Mol Med Rep 12: 5423-5428, 2015.
APA
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., & Zhang, T. (2015). Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts. Molecular Medicine Reports, 12, 5423-5428. https://doi.org/10.3892/mmr.2015.4080
MLA
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., Zhang, T."Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts". Molecular Medicine Reports 12.4 (2015): 5423-5428.
Chicago
Li, X., Luo, X., Li, K., Wang, N., Hua, E., Zhang, Y., Zhang, T."Difference in protective effects of three structurally similar flavonoid glycosides from Hypericum ascyron against H2O2-induced injury in H9c2 cardiomyoblasts". Molecular Medicine Reports 12, no. 4 (2015): 5423-5428. https://doi.org/10.3892/mmr.2015.4080
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