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Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro

  • Authors:
    • Qunchuan Zhuang
    • Jing Li
    • Youqin Chen
    • Jiumao Lin
    • Faze Lai
    • Xuzheng Chen
    • Xindeng Lin
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Department of Medical Technology, Fujian Health College, Fuzhou, Fujian 350101, P.R. China, Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA, Department of Pharmacology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China
    Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4851-4858
    |
    Published online on: July 16, 2015
       https://doi.org/10.3892/mmr.2015.4086
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Abstract

The widely-used Chinese medicinal herb Hypericum japonicum, also known as Hypericum japonicum Thunb or Tianjihuang, displays potent anti‑carcinogenic effects against liver cancer. However, the molecular mechanism underlying the therapeutic effects of Hypericum japonicum remains to be elucidated. The present study investigated the in vivo efficacy of ethyl acetate extract of Hypericum japonicum (EAEHJ) against tumor growth in an H22 cell‑bearing liver cancer mouse model. Treatment with EAEHJ significantly reduced tumor weight, but had no effect on murine body weight. The results of the present study also showed that EAEHJ induced H22 cell apoptosis in vivo. In addition, the anti‑carcinogenic effects of EAEHJ were investigated in vitro. The results of the present study demonstrate that both phospholipid asymmetry in the plasma membrane and mitochondrial membrane potential were deregulated in HepG2 human hepatoma cells, following treatment with EAEHJ. Treatment with EAEHJ also increased the ratio of pro‑apoptotic B‑cell lymphoma 2 (Bcl‑2)‑associated X protein (Bax) to anti‑apoptotic Bcl‑2, and activated the caspase‑9 signaling pathway. These results suggest that EAEHJ is able to trigger the apoptosis of liver cancer cells via the mitochondria-dependent pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zhuang Q, Li J, Chen Y, Lin J, Lai F, Chen X, Lin X and Peng J: Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro. Mol Med Rep 12: 4851-4858, 2015.
APA
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X. ... Peng, J. (2015). Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro. Molecular Medicine Reports, 12, 4851-4858. https://doi.org/10.3892/mmr.2015.4086
MLA
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X., Lin, X., Peng, J."Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro". Molecular Medicine Reports 12.4 (2015): 4851-4858.
Chicago
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X., Lin, X., Peng, J."Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro". Molecular Medicine Reports 12, no. 4 (2015): 4851-4858. https://doi.org/10.3892/mmr.2015.4086
Copy and paste a formatted citation
x
Spandidos Publications style
Zhuang Q, Li J, Chen Y, Lin J, Lai F, Chen X, Lin X and Peng J: Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro. Mol Med Rep 12: 4851-4858, 2015.
APA
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X. ... Peng, J. (2015). Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro. Molecular Medicine Reports, 12, 4851-4858. https://doi.org/10.3892/mmr.2015.4086
MLA
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X., Lin, X., Peng, J."Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro". Molecular Medicine Reports 12.4 (2015): 4851-4858.
Chicago
Zhuang, Q., Li, J., Chen, Y., Lin, J., Lai, F., Chen, X., Lin, X., Peng, J."Ethyl acetate extract of Hypericum japonicum induces apoptosis via the mitochondria-dependent pathway in vivo and in vitro". Molecular Medicine Reports 12, no. 4 (2015): 4851-4858. https://doi.org/10.3892/mmr.2015.4086
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