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Article

Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts

  • Authors:
    • Toshihisa Maeda
    • Yasushi Miura
    • Koji Fukuda
    • Shinya Hayashi
    • Masahiro Kurosaka
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Chuo, Kobe, Hyogo 650‑0017, Japan
  • Pages: 5191-5196
    |
    Published online on: July 20, 2015
       https://doi.org/10.3892/mmr.2015.4097
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Abstract

Decoy receptor 3 (DcR3) is expressed in rheumatoid arthritis fibroblast‑like synoviocytes (RA‑FLS) and downregulates the expression of tryptophan hydroxylase 1 (TPH1), which is the rate‑limiting enzyme in serotonin synthesis. The aim of the present study was to determine the specificity of the effects of DcR3 on TPH1 in RA‑FLS, and therefore determine whether DcR3 had the potential to modulate the pathogenesis of RA. The present study also aimed to compare the effects of DcR3 and inflammatory cytokines on the expression of TPH1 in RA‑FLS and osteoarthritis (OA)‑FLS. Primary cultured RA‑ or OA‑FLS were incubated with 1.0 µg/ml DcR3‑Fc protein or 1.0 µg/ml control immunoglobulin G (IgG)1 for 12 h, or with 1.0 ng/ml tumor necrosis factor (TNF)α, 1.0 ng/ml interleukin (IL)‑1β or serum‑free Opti‑MEM only, for 24 h. The relative mRNA expression levels of TPH1 were subsequently quantified using reverse transcription‑polymerase chain reaction. The expression of serotonin in RA or OA synovial tissue was detected using immunohistochemistry. The mRNA expression of TPH1 was observed in both RA‑ and OA‑FLS and was significantly decreased following treatment with DcR3 in the RA‑FLS, however, not in the OA‑FLS. The mRNA expression of TPH1 was significantly decreased following treatment with TNFα or IL‑1β in both the RA‑ and OA‑FLS. The expression of serotonin in the multi‑layered lining synovial cells of RA and the outer layer lining synovial cells of OA was detected using immunohistochemistry. The present study is the first, to the best of our knowledge, to demonstrate that the expression of TPH1 in FLS is downregulated by inflammatory cytokines, and that DcR3 suppressed the expression of TPH1 in RA‑FLS in a disease‑specific manner. These results suggested that synovial serotonin may be involved in the pathogenesis of RA, and that TPH1 and DcR3 may be potential therapeutic targets for the treatment of RA.
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Copy and paste a formatted citation
Spandidos Publications style
Maeda T, Miura Y, Fukuda K, Hayashi S and Kurosaka M: Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts. Mol Med Rep 12: 5191-5196, 2015.
APA
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., & Kurosaka, M. (2015). Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts. Molecular Medicine Reports, 12, 5191-5196. https://doi.org/10.3892/mmr.2015.4097
MLA
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts". Molecular Medicine Reports 12.4 (2015): 5191-5196.
Chicago
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts". Molecular Medicine Reports 12, no. 4 (2015): 5191-5196. https://doi.org/10.3892/mmr.2015.4097
Copy and paste a formatted citation
x
Spandidos Publications style
Maeda T, Miura Y, Fukuda K, Hayashi S and Kurosaka M: Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts. Mol Med Rep 12: 5191-5196, 2015.
APA
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., & Kurosaka, M. (2015). Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts. Molecular Medicine Reports, 12, 5191-5196. https://doi.org/10.3892/mmr.2015.4097
MLA
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts". Molecular Medicine Reports 12.4 (2015): 5191-5196.
Chicago
Maeda, T., Miura, Y., Fukuda, K., Hayashi, S., Kurosaka, M."Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts". Molecular Medicine Reports 12, no. 4 (2015): 5191-5196. https://doi.org/10.3892/mmr.2015.4097
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