Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2015 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2015 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells

  • Authors:
    • Xiaodie Zhang
    • Ke Xue
    • Jia Zhou
    • Peng Xu
    • Huizhen Huang
    • Kai Liu
  • View Affiliations / Copyright

    Affiliations: Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
  • Pages: 5355-5360
    |
    Published online on: July 22, 2015
       https://doi.org/10.3892/mmr.2015.4113
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Repair of cartilage defects remains a challenge for surgeons, owing to its poor self‑repairing capacity. Cartilage tissue engineering, particularly marrow stem cell‑based cartilage regeneration, provides a promising option for the regeneration of damaged cartilage. Although producing tissue‑engineered cartilage from marrow stem cells appeared to be a feasible method, constructing certain sub‑types of cartilage, including elastic cartilage, remains difficult. Therefore, the present study explored the feasibility of constructing elastic cartilage by culturing bone marrow‑derived stem cells (BMSCs) in the supernatant of elastic cartilage cells to generate elastic cartilage. The elastic cartilage cells were obtained from the auricle cartilage of a newborn pig, and BMSCs were isolated from pig bone marrow aspirate. The supernatant of the chondrocytes was collected and then used to the culture BMSCs. At various time‑points, the differentiation of BMSCs was evaluated by gross view, histological examination and quantitative polymerase chain reaction. BMSCs changed from spindle‑shaped cells into polygonal cells with increasing culture time. The expression of collagen II and elastin was observed in the cells cultured in the supernatant of elastic chondrocytes, while no expression was observed in the control cells. Furthermore, the expression of collagen I and collagen X was downregulated in the cells cultured in the supernatant of elastic cartilage cells. The supernatant of elastic cartilage cells promoted the differentiation of BMSCs into elastic cartilage cells, which may be a promising method for constructing certain sub‑types of tissue‑engineered cartilage.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Hwang CM, Lee BK, Green D, Jeong SY, Khang G, Jackson JD, Atala A, Lee SJ and Yoo JJ: Auricular reconstruction using tissue-engineered alloplastic implants for improved clinical outcomes. Plast Reconstr Surg. 133:360e–369e. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Wachsmuth L, Söder S, Fan Z, Finger F and Aigner T: Immunolocalization of matrix proteins in different human cartilage subtype. Histol Histopathol. 21:477–485. 2006.PubMed/NCBI

3 

Suutarla S, Rautio J and Klockars T: The learning curve in microtia surgery. Facial Plast Surg. 25:164–168. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Wilkes GH: Learning to perform ear reconstruction. Facial Plast Surg. 25:158–163. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Bichara DA, O'Sullivan NA, Pomerantseva I, et al: The tissue-engineered auricle: past, present and future. Tissue Eng Part B Rev. 18:51–61. 2012. View Article : Google Scholar

6 

Reiffel AJ, Kafka C, Hernandez KA, et al: High-fidelity tissue engineering of patient-specific auricles for reconstruction of pediatric microtia and other auricular deformities. PLoS One. 8:e565062013. View Article : Google Scholar : PubMed/NCBI

7 

Sterodimas A, de Faria J, Correa WE and Pitanguy I: Tissue engineering and auricular reconstruction: a review. J Plast Reconstr Aesthet Surg. 62:447–452. 2009. View Article : Google Scholar

8 

Nayyer L, Patel KH, Esmaeili A, et al: Tissue engineering: revolution and challenge in auricular cartilage reconstruction. Plast Reconstr Surg. 129:1123–1137. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Patel KH, Nayyer L and Seifalian AM: Chondrogenic potential of bone marrow-derived mesenchymal stem cells on a novel, auricular-shaped, nanocomposite scaffold. J Tissue Eng. 4:20417314135167822013. View Article : Google Scholar

10 

Pittenger MF, Mackay AM, Beck SC, et al: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Abrahamsson CK, Yang F, Park H, et al: Chondrogenesis and mineralization during in vitro culture of human mesenchymal stem cells on three-dimensional woven scaffolds. Tissue Eng Part A. 16:3709–3718. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Mueller MB and Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells. Arthritis Rheum. 58:1377–1388. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Pelttari K, Winter A, Steck E, et al: Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice. Arthritis Rheum. 54:3254–3266. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Qing C, Wei-ding C and Wei-min F: Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components. Braz J Med Biol Res. 44:303–310. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Zhang L, He A, Yin Z, et al: Regeneration of human-ear-shaped cartilage by co-culturing human microtia chondrocytes with BMSCs. Biomaterials. 35:4878–4887. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Acharya C, Adesida A, Zajac P, et al: Enhanced chondrocyte proliferation and mesenchymal stromal cells chondrogenesis in coculture pellets mediate improved cartilage formation. J Cell Physiol. 227:88–97. 2012. View Article : Google Scholar

17 

Fischer J, Dickhut A, Rickert M and Richter W: Human articular chondrocytes secrete parathyroid hormone-related protein and inhibit hypertrophy of mesenchymal stem cells in coculture during chondrogenesis. Arthritis Rheum. 62:2696–2706. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Hwang NS, Varghese S, Puleo C, Zhang Z and Elisseeff J: Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. J Cell Physiol. 212:281–284. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Xue K, Zhu Y, Zhang Y, Chiang C, Zhou G and Liu K: Xenogeneic chondrocytes promote stable subcutaneous chondrogenesis of bone marrow-derived stromal cells. Int J Mol Med. 29:146–152. 2012.

20 

Friedenstein AJ, Chailakhjan RK and Lalykina KS: The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 3:393–403. 1970.PubMed/NCBI

21 

Xu LQ, Huang Y-W, Luo R-Z and Zhang Y-N: Establishment of the retroperitoneal lymph node metastasis model of endometrial VX2 carcinoma in rabbits and observation of its metastatic features. World J Surg Oncol. 13:1092015. View Article : Google Scholar : PubMed/NCBI

22 

Xue K, Qi L, Zhou G and Liu K: A two-step method of constructing mature cartilage using bone marrow-derived mesenchymal stem cells. Cells Tissues Organs. 197:484–495. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Zheng B, Jiang J, Luo K, Liu L, Lin M, Chen Y and Yan F: Increased osteogenesis in osteoporotic bone marrow stromal cells by overexpression of leptin. Cell Tissue Res. 2015.Epub ahead of print. View Article : Google Scholar : PubMed/NCBI

24 

Bian L, Zhai DY, Mauck RL and Burdick JA: Coculture of human mesenchymal stem cells and articular chondrocytes reduces hypertrophy and enhances functional properties of engineered cartilage. Tissue Eng Part A. 17:1137–1145. 2011. View Article : Google Scholar :

25 

He F, Chen X and Pei M: Reconstruction of an in vitro tissue-specific microenvironment to rejuvenate synovium-derived stem cells for cartilage tissue engineering. Tissue Eng Part A. 15:3809–3821. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Vinardell T, Thorpe SD, Buckley CT and Kelly DJ: Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model. Ann Biomed Eng. 37:2556–2565. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Yang YH, Lee AJ and Barabino GA: Coculture-driven mesenchymal stem cell-differentiated articular chondrocyte-like cells support neocartilage development. Stem Cells Transl Med. 1:843–854. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Lu T, Huang Y, Wang H, Ma Y and Guan W: Multi-lineage potential research of bone marrow-derived stromal cells (BMSCs) from cattle. Appl Biochem Biotechnol. 172:21–35. 2014. View Article : Google Scholar

29 

Diaz-Romero J, Gaillard JP, Grogan SP, Nesic D, Trub T and Mainil-Varlet P: Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture. J Cell Physiol. 202:731–742. 2005. View Article : Google Scholar

30 

Albrecht C, Tichy B, Nurnberger S, et al: Gene expression and cell differentiation in matrix-associated chondrocyte transplantation grafts: a comparative study. Osteoarthritis Cartilage. 19:1219–1227. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Potier E, Rivron NC, Van Blitterswijk CA and Ito K: Micro-aggregates do not influence bone marrow stromal cell chondrogenesis. J Tissue Eng Regen Med. Apr 2–2014.Epub ahead of print. View Article : Google Scholar : PubMed/NCBI

32 

Leppaluoto J, Zeytin F, Ueno N, Ying SY, Ling N and Guillemin R: Myelin basic protein present in the acid extracts of rat hypothalami releases insulin and glucagon from isolated rat pancreatic islets. Acta Physiol Scand. 134:253–261. 1988. View Article : Google Scholar : PubMed/NCBI

33 

Zuo Q, Cui W, Liu F, Wang Q, Chen Z and Fan W: Co-cultivated mesenchymal stem cells support chondrocytic differentiation of articular chondrocytes. Int Orthop. 37:747–752. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Little CJ, Bawolin NK and Chen X: Mechanical properties of natural cartilage and tissue-engineered constructs. Tissue Eng Part B Rev. 17:213–227. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Kuhne M, John T, El-Sayed K, et al: Characterization of auricular chondrocytes and auricular/articular chondrocyte co-cultures in terms of an application in articular cartilage repair. Int J Mol Med. 25:701–708. 2010.PubMed/NCBI

36 

Hong E and Reddi AH: Dedifferentiation and redifferentiation of articular chondrocytes from surface and middle zones: changes in microRNAs-221/-222, -140 and -143/145 expression. Tissue Eng Part A. 19:1015–1022. 2013. View Article : Google Scholar

37 

Cheng T, Maddox NC, Wong AW, Rahnama R and Kuo AC: Comparison of gene expression patterns in articular cartilage and dedifferentiated articular chondrocytes. J Orthop Res. 30:234–245. 2012. View Article : Google Scholar

38 

von der Mark K, Gauss V, von der Mark H and Müller P: Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature. 267:531–532. 1977. View Article : Google Scholar : PubMed/NCBI

39 

Mizuno M, Takebe T, Kobayashi S, et al: Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes. Transplant Proc. 46:1217–1221. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Xue K, Zhou J, Xu P, Huang H and Liu K: Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells. Mol Med Rep 12: 5355-5360, 2015.
APA
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., & Liu, K. (2015). Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells. Molecular Medicine Reports, 12, 5355-5360. https://doi.org/10.3892/mmr.2015.4113
MLA
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., Liu, K."Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells". Molecular Medicine Reports 12.4 (2015): 5355-5360.
Chicago
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., Liu, K."Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells". Molecular Medicine Reports 12, no. 4 (2015): 5355-5360. https://doi.org/10.3892/mmr.2015.4113
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Xue K, Zhou J, Xu P, Huang H and Liu K: Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells. Mol Med Rep 12: 5355-5360, 2015.
APA
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., & Liu, K. (2015). Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells. Molecular Medicine Reports, 12, 5355-5360. https://doi.org/10.3892/mmr.2015.4113
MLA
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., Liu, K."Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells". Molecular Medicine Reports 12.4 (2015): 5355-5360.
Chicago
Zhang, X., Xue, K., Zhou, J., Xu, P., Huang, H., Liu, K."Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells". Molecular Medicine Reports 12, no. 4 (2015): 5355-5360. https://doi.org/10.3892/mmr.2015.4113
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team