Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2015 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2015 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress

  • Authors:
    • Shasha Hong
    • Li Hong
    • Debin Wu
    • Bingshu Li
    • Cheng Liu
    • Wenjun Guo
    • Jie Min
    • Ming Hu
    • Yang Zhao
    • Qing Yang
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 5342-5348
    |
    Published online on: July 23, 2015
       https://doi.org/10.3892/mmr.2015.4115
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to explore the underlying mechanisms of the roles of mechanical factors in the pathogenesis of pelvic organ prolapse (POP). The experiments were performed on fibroblasts derived from uterosacral ligaments and cardinal ligaments of patients who received total hysterectomy due to benign disease excluding POP. Fibroblasts were cultured after collagenase digestion and identified by morphological observation and immunocytochemical methods. A four‑point bending device was used to subject fibroblasts at passage 4‑6 to strains of 0, 1,333 µ (1 mm), 2,666 µ (2 mm) or 5,333 µ (4 mm) at a frequency of 0.1 Hz for 4 h. Intracellular reactive oxygen species (ROS) were quantified using the fluorescent probe 2',7'‑dichlorodihydrofluorescein diacetate. Changes in the mitochondrial membrane potential were verified using the fluorescent dye JC‑1, and apoptosis was detected using Annexin V/propidium iodide staining and flow cytometric analysis. Mechanical strain changed the morphology and adherence ability of parametrial ligament fibroblasts. Furthermore, the production of ROS was significantly increased and the mitochondrial membrane potential obviously declined with the enhancement of mechanical stress loading. In addition, the apoptotic rate of fibroblasts subjected to high mechanical strain was significantly increased compared with that in fibroblast under low‑intensity strain. In conclusion, the present study showed that mechanical strain enhanced intracellular ROS levels, decreased the mitochondrial membrane potential and increased the apoptotic rate in human parametrial ligament fibroblasts, which may contribute to POP.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Peng P, Zhu L, Lang J, Wang WY and Shi HH: Unilateral sacrospinous ligament fixation for treatment of genital prolapse. Chin Med J (Engl). 123:19952010.

2 

Jelovsek JE, Maher C and Barber MD: Pelvic organ prolapse. Lancet. 369:1027–1038. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Wondrak GT, Roberts MJ, Jacobson MK and Jacobson EL: 3-hydroxypyridine chromophores are endogenous sensitizers of photooxidative stress in human skin cells. J Biol Chem. 279:30009–30020. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Mizutari K, Ono T, Ikeda K, Kayashima K and Horiuchi S: Photo-enhanced modification of human skin elastin in actinic elastosis by N(epsilon)-(carboxymethyl) lysine, one of the glycoxidation products of the maillard reaction. J Invest Dermatol. 108:797–802. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Lu D and Kassab GS: Role of shear stress and stretch in vascular mechanobiology. J Roy Soc Interface. 8:1379–1385. 2011. View Article : Google Scholar

6 

Tan JL: Cell proliferation, differentiation, apoptosis under a serial stretches and the underlying mechanisms. PhD dissertation. The Fourth Military Medical University; Xi'an: 2010

7 

Choy KW, Liu YM, Chu CY, Wang CC, Lui WT, Lee LL, Pang MW, Rogers MS and Yip SK: High isoprostane level in cardinal ligament-derived fibroblasts and urine sample of women with uterine prolapse. BJOG. 115:1179–1183. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Visco AG and Yuan L: Differential gene expression in pubococ-cygeus muscle from patients with pelvic organ prolapse. Am J Obstet Gynecol. 189:102–112. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Makpol S, Azura Jam F, Anum Mohd Yusof Y and Zurinah Wan Ngah W: Modulation of collagen synthesis and its gene expression in human skin fibroblasts by tocotrienol-rich fraction. Arch Med Sci. 7:889–895. 2011. View Article : Google Scholar

10 

Kim E J, Chung N, Park SH, Lee KH, Kim SW, Kim JY, Bai SW and Jeon MJ: Involvement of oxidative stress and mitochondrial apoptosis in the pathogenesis of pelvic organ prolapse. J Urol. 189:588–594. 2013. View Article : Google Scholar

11 

Wen Y, Ho JY, Polan ML and Chen B: Expression of apoptotic factors in vaginal tissues from women with urogenital prolapse. Neurourol and Urodynam. 30:1627–1632. 2011. View Article : Google Scholar

12 

Bao R, Liu X and Zhou J: Primary culture and identification of human fibroblasts. J XinX Med Coll. 5:0152011.In Chinese.

13 

Chow D and Rodríguez LV: Epidemiology and prevalence of pelvic organ prolapse. Curr Opin Urol. 23:293–298. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Jean-Charles C, Rubod C, Brieu M, Boukerrou M, Fasel J and Cosson M: Biomechanical properties of prolapsed or non-prolapsed vaginal tissue: impact on genital prolapse surgery. Int Urogynecol J. 21:1535–1538. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Lang JH and Zhang XD: Obstetrics and Gynecology Clinical Anatomy. 2010 edition. Shandong Science and Technology Press; Jinan: pp. 692010

16 

Ewies AA, Elshafie M, Li J, Stanley A, Thompson J, Styles J, White I and Al-Azzawi F: Changes in transcription profile and cytoskeleton morphology in pelvic ligament fibroblasts in response to stretch: The effects of estradiol and levormeloxifene. Mol Human Reprod. 14:127–135. 2008. View Article : Google Scholar

17 

Li XN: Study on the methods and platform of image processing for cellular biomechanical experiments. PhD dissertation. Sichuan University; Chengdu: 2003

18 

Fransen M, Nordgren M, Wang B and Apanasets O: Role of peroxisomes in ROS/RNS-metabolism: Implications for human disease. Biochim Biophys Acta. 1822:1363–1373. 2012. View Article : Google Scholar

19 

Grote K, Flach I, Luchtefeld M, Akin E, Holland SM, Drexler H and Schieffer B: Mechanical stretch enhances mRNA expression and proenzyme release of matrix metalloproteinase-2 (MMP-2) via NAD (P) H oxidase-derived reactive oxygen species. Circ Res. 92:e80–e86. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Sinha K, Das J, Pal PB and Sil PC: Oxidative stress: The mitochondria-dependent and mitochondria-independent pathways of apoptosis. Arch Toxicol. 87:1157–1180. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Teshima Y, Takahashi N, Thuc LC, Nishio S, Nagano-Torigoe Y, Miyazaki H, Ezaki K, Yufu K, Hara M, Nakagawa M and Saikawa T: High-glucose condition reduces cardioprotective effects of insulin against mechanical stress-induced cell injury. Life Sci. 87:154–161. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Choudhary R, Baker KM and Pan J: All-trans retinoic acid prevents angiotensin II- and mechanical stretch-induced reactive oxygen species generation and cardiomyocyte apoptosis. J Cell Physiol. 215:172–181. 2008. View Article : Google Scholar

23 

Meyer T, Meyer U, Stratmann U, Wiesmann HP and Joos U: Identification of apoptotic cell death in distraction osteogenesis. Cell Biol Int. 23:439–446. 1999. View Article : Google Scholar

24 

Ning QM and Qang XR: Early apoptosis induced by mechanical stretch in human alveolar typeIIepithelial cells. Journal of Shanghai Jiao Tong University (Medical Science Edition). 27:961–964. 2007.

25 

Li H, Zhang XY, Wu TJ, Cheng W, Liu X, Jiang TT, Wen J, Li J, Ma QL and Hua ZC: Endoplasmic reticulum stress regulates rat mandibular cartilage thinning under compressive mechanical stress. J Biol Chem. 288:18172–18183. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Ding F, Shao ZW, Yang SH, Wu Q, Gao F and Xiong LM: Role of mitochondrial pathway in compression-induced apoptosis of nucleus pulposus cells. Apoptosis. 17:579–590. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Miao YL, Hu CH, Wang JL, et al: The preliminary study on biomechanical properties of posterior vaginal wall tissue of the patient with rectocele. Chinese J Clin Obstet Gynecol. 9:180–183. 2008.In Chinese.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hong S, Hong L, Wu D, Li B, Liu C, Guo W, Min J, Hu M, Zhao Y, Yang Q, Yang Q, et al: Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress. Mol Med Rep 12: 5342-5348, 2015.
APA
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W. ... Yang, Q. (2015). Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress. Molecular Medicine Reports, 12, 5342-5348. https://doi.org/10.3892/mmr.2015.4115
MLA
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W., Min, J., Hu, M., Zhao, Y., Yang, Q."Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress". Molecular Medicine Reports 12.4 (2015): 5342-5348.
Chicago
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W., Min, J., Hu, M., Zhao, Y., Yang, Q."Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress". Molecular Medicine Reports 12, no. 4 (2015): 5342-5348. https://doi.org/10.3892/mmr.2015.4115
Copy and paste a formatted citation
x
Spandidos Publications style
Hong S, Hong L, Wu D, Li B, Liu C, Guo W, Min J, Hu M, Zhao Y, Yang Q, Yang Q, et al: Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress. Mol Med Rep 12: 5342-5348, 2015.
APA
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W. ... Yang, Q. (2015). Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress. Molecular Medicine Reports, 12, 5342-5348. https://doi.org/10.3892/mmr.2015.4115
MLA
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W., Min, J., Hu, M., Zhao, Y., Yang, Q."Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress". Molecular Medicine Reports 12.4 (2015): 5342-5348.
Chicago
Hong, S., Hong, L., Wu, D., Li, B., Liu, C., Guo, W., Min, J., Hu, M., Zhao, Y., Yang, Q."Oxidative damage to human parametrial ligament fibroblasts induced by mechanical stress". Molecular Medicine Reports 12, no. 4 (2015): 5342-5348. https://doi.org/10.3892/mmr.2015.4115
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team