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Article

Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure

  • Authors:
    • Yubyeol Jeon
    • Junchul David Yoon
    • Lian Cai
    • Seon‑Ung Hwang
    • Eunhye Kim
    • Zhong Zheng
    • Euibae Jeung
    • Eunsong Lee
    • Sang‑Hwan Hyun
  • View Affiliations / Copyright

    Affiliations: Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea, Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 361‑763, Republic of Korea, Laboratory of Theriogenology, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-710, Republic of Korea
  • Pages: 5973-5982
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    Published online on: July 27, 2015
       https://doi.org/10.3892/mmr.2015.4125
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Abstract

The present study investigated the effects of zinc deficiency during in vitro maturation (IVM) of porcine oocytes. Zinc deficiency was induced by administering the membrane‑permeable zinc chelator N,N,N',N'‑tetrakis‑(2‑pyridylmethyl)‑ethylendiamine (TPEN). First, the effects of zinc deficiency during IVM on a TPEN‑treated group and a TPEN+zinc-treated group compared with a control group were assessed. The oocyte maturation rates and subsequent embryonic developmental competence of the TPEN+zinc‑treated oocytes were similar to those of the control oocytes (metaphase II [MII] rate, 93.0 and 92.7%, respectively, and blastocyst [BL] formation rate, 42.0 and 40.0%, respectively). These results were significantly different from those obtained for the TPEN‑treated oocytes (MII rate, 0.61%; BL formation rate, 0%). Although the TPEN‑treated oocytes were arrested at metaphase I (MI), the distribution of microtubules was normal. However, microfilament formation was abnormal in the TPEN‑treated oocytes. Furthermore, the effect of a temporary zinc deficiency during IVM on oocyte maturation and subsequent embryonic development was assessed. TPEN (10 µM) was added to the IVM medium for 0, 7, 15 or 22 h. The 0 h‑treated oocytes showed an 83.9% MII rate, while the 7 h‑treated oocytes had a significantly lower MII rate (44.8%). Most of the 15- and 22 h‑treated oocytes were arrested at MI (MI rate: 98.0 and 97.2%, respectively; MII rate, 0% in both groups). Reductions in the BL formation were dependent on the TPEN treatment duration (29.3, 9.2, 0, and 0% after 0, 7, 15 and 22 h, respectively). In conclusion, zinc is an essential element for successful oocyte maturation and embryonic development in pigs. Zinc deficiency caused a meiotic block and had lasting effects on early embryonic development.
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Copy and paste a formatted citation
Spandidos Publications style
Jeon Y, Yoon JD, Cai L, Hwang SU, Kim E, Zheng Z, Jeung E, Lee E and Hyun SH: Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure. Mol Med Rep 12: 5973-5982, 2015.
APA
Jeon, Y., Yoon, J.D., Cai, L., Hwang, S., Kim, E., Zheng, Z. ... Hyun, S. (2015). Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure. Molecular Medicine Reports, 12, 5973-5982. https://doi.org/10.3892/mmr.2015.4125
MLA
Jeon, Y., Yoon, J. D., Cai, L., Hwang, S., Kim, E., Zheng, Z., Jeung, E., Lee, E., Hyun, S."Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure". Molecular Medicine Reports 12.4 (2015): 5973-5982.
Chicago
Jeon, Y., Yoon, J. D., Cai, L., Hwang, S., Kim, E., Zheng, Z., Jeung, E., Lee, E., Hyun, S."Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure". Molecular Medicine Reports 12, no. 4 (2015): 5973-5982. https://doi.org/10.3892/mmr.2015.4125
Copy and paste a formatted citation
x
Spandidos Publications style
Jeon Y, Yoon JD, Cai L, Hwang SU, Kim E, Zheng Z, Jeung E, Lee E and Hyun SH: Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure. Mol Med Rep 12: 5973-5982, 2015.
APA
Jeon, Y., Yoon, J.D., Cai, L., Hwang, S., Kim, E., Zheng, Z. ... Hyun, S. (2015). Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure. Molecular Medicine Reports, 12, 5973-5982. https://doi.org/10.3892/mmr.2015.4125
MLA
Jeon, Y., Yoon, J. D., Cai, L., Hwang, S., Kim, E., Zheng, Z., Jeung, E., Lee, E., Hyun, S."Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure". Molecular Medicine Reports 12.4 (2015): 5973-5982.
Chicago
Jeon, Y., Yoon, J. D., Cai, L., Hwang, S., Kim, E., Zheng, Z., Jeung, E., Lee, E., Hyun, S."Zinc deficiency during in vitro maturation of porcine oocytes causes meiotic block and developmental failure". Molecular Medicine Reports 12, no. 4 (2015): 5973-5982. https://doi.org/10.3892/mmr.2015.4125
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