Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays

Li,Xiao‑Αn; Zhou,Yan; Zhou,Shu‑Χian; Liu,Hai‑Rong; Xu,Jin‑Mei; Gao,Long; Yu,Xian‑Jing; Li,Xiao‑Hui

doi:10.3892/mmr.2015.4126

October-2015 Volume 12 Issue 4

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October-2015 Volume 12 Issue 4

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Article Open Access

Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays

Authors:
- Xiao‑Αn Li
- Yan Zhou
- Shu‑Χian Zhou
- Hai‑Rong Liu
- Jin‑Mei Xu
- Long Gao
- Xian‑Jing Yu
- Xiao‑Hui Li
View Affiliations / Copyright

Affiliations: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China

Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5807-5815
|
Published online on: July 27, 2015

https://doi.org/10.3892/mmr.2015.4126
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Abstract

Melanosis coli (MC) refers to the condition characterized by abnormal brown or black pigmentation deposits on the colonic mucosa. However, the histopathological findings and genes associated with the pathogenesis of melanosis coli remain to be fully elucidated. The present study aimed to examine the histopathological features and differentially expressed genes of MC. This involved performing hematoxylin and eosin staining, specific staining and immunohistochemistry on tissues sections, which were isolated from patients diagnosed with MC. DNA expression microarray analysis, western blotting and immunofluorescence assays were performed to analyze the differentially expressed genes of melanosis coli. The results demonstrated that the pigment deposits in MC consisted of lipofuscin. A TUNEL assay revealed that a substantial number of apoptotic cells were present within the macrophages and superficial lamina propria of the colonic epithelium. Expression microarray analysis revealed that the significantly downregulated genes were CYP3A4, CYP3A7, UGT2B11 and UGT2B15 in melanosis coli. Western blotting and immunofluorescence assays indicated that the expression of CYP3A4 in the normal tissue was higher than in the MC tissue. The results of the present study provided a comprehensive description of the histopathological characteristics and pathogenesis of MC and for the first time, to the best of our knowledge, demonstrated that the cytochrome P450‑associated genes were significantly downregulated in melanosis coli. This novel information can be used to assist in further investigations of melanosis coli.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Histopathology of melanosis coli and determination of its associated genes by comparative analysis of expression microarrays

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