16S rDNA PCR-DGGE and sequencing in the diagnosis of neonatal late-onset septicemia

Tsai M-H, Hsu JF, Chu SM, Lien R, Huang HR, Chiang MC, Fu RH, Lee CW and Huang YC: Incidence, clinical characteristics and risk factors for adverse outcome in neonates with late-onset sepsis. Pediatr Infect Dis J. 33:e7–e13. 2014. View Article : Google Scholar

Stoll BJ, Hansen N, Fanaroff AA, Wright LL, Carlo WA, Ehrenkranz RA, Lemons JA, Donovan EF, Stark AR, Tyson JE, et al: Late-onset sepsis in very low birth weight neonates: The experience of the NICHD neonatal research network. Pediatrics. 110:285–291. 2002. View Article : Google Scholar : PubMed/NCBI

Rubin LG, Sánchez PJ, Siegel J, Levine G, Saiman L and Jarvis WR; Pediatric Prevention Network: Evaluation and treatment of neonates with suspected late-onset sepsis: A survey of neonatologists' practices. Pediatrics. 110:e422002. View Article : Google Scholar : PubMed/NCBI

Paolucci M, Landini MP and Sambri V: Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents. 36(Suppl 2): S6–S16. 2010. View Article : Google Scholar : PubMed/NCBI

Peters RP, van Agtmael MA, Danner SA, Savelkoul PH and Vandenbroucke-Grauls CM: New developments in the diagnosis of blood stream infections. Lancet Infect Dis. 4:751–760. 2004. View Article : Google Scholar : PubMed/NCBI

Mancini N, Carletti S, Ghidoli N, Cichero P, Ossi CM, Ieri R, Poli E, Burioni R and Clementi M: Molecular diagnosis of polymicrobial sepsis. J Clin Microbiol. 47:1274–1275. 2009. View Article : Google Scholar : PubMed/NCBI

Sontakke S, Cadenas MB, Maggi RG, Diniz PP and Breitschwerdt EB: Use of broad range16S rDNA PCR in clinical microbiology. J Microbiol Methods. 76:217–225. 2009. View Article : Google Scholar

Ley BE, Linton CJ, Bennett DM, Jalal H, Foot AB and Millar MR: Detection of bacteraemia in patients with fever and neutropenia using 16S rRNA gene amplification by polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 17:247–253. 1998. View Article : Google Scholar : PubMed/NCBI

Ng PC, Cheng SH, Chui KM, Fok TF, Wong MY, Wong W, Wong RP and Cheung KL: Diagnosis of late onset neonatal sepsis with cytokines, adhesion molecule and C-reactive protein in preterm very low birthweight infants. Arch Dis Child Fetal Neonatal Ed. 77:F221–F227. 1997. View Article : Google Scholar

Yu Z and Morrison M: Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol. 70:4800–4806. 2004. View Article : Google Scholar : PubMed/NCBI

Muyzer G, de Waal EC and Uitterlinden AG: Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol. 59:695–700. 1993.PubMed/NCBI

Pammi M, Flores A, Leeflang M and Versalovic J: Molecular assays in the diagnosis of neonatal sepsis: A systematic review and meta-analysis. Pediatrics. 128:e973–e985. 2011. View Article : Google Scholar : PubMed/NCBI

Jordan JA, Butchko AR and Durso MB: Use of pyrosequencing of 16S rRNA fragments to differentiate between bacteria responsible for neonatal sepsis. J Mol Diagn. 7:105–110. 2005. View Article : Google Scholar : PubMed/NCBI

Jordan JA and Durso MB: Comparison of 16S rRNA gene PCR and BACTEC 9240 for detection of neonatal bacteremia. J Clin Microbiol. 38:2574–2578. 2000.PubMed/NCBI