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Article

Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor

  • Authors:
    • Zhi Cheng Xu
    • Qun Zhang
    • Hong Li
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, P.R. China, Department of Life Information and Instrument Engineering, Hangzhou Electronic Science and Technology University, Hangzhou, Zhejiang 310058, P.R. China
  • Pages: 6005-6012
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    Published online on: July 29, 2015
       https://doi.org/10.3892/mmr.2015.4147
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Abstract

Bone marrow‑derived cells (BMCs) have demonstrated their ability to differentiate into multiple cell lineages and may be a promising cell source for vascular tissue engineering. Although much progress has been made in the engineering of small blood vessels (<6 mm in diameter) with biodegradable materials such as polyglycolic acid (PGA), it remains a challenge to engineer large vessels (>6 mm in diameter) due to unsatisfactory biomechanical properties. The present study was to engineered an elastic large vessel wall (6 mm in diameter) using a PGA unwoven fibre scaffold covered with BMCs from canine humeri. The cell‑PGA sheet was then loaded into a bioreactor designed for the present study, with dynamic pulsatile culture conditions to mimic the physiological vessel environment. After four weeks of the pulsatile stimuli culture, an elastic vessel wall was formed. Histological analyses demonstrated that layers of smooth muscle‑like cells and well‑oriented collagenous fibres were evenly oriented in the dynamic group. By contrast, disorganised cells and randomly collagenous fibres were apparent in the static group. Furthermore, the engineered vessel wall in the dynamic group exhibited significantly improved biomechanical properties compared with those in static culture group. The approach developed in the present study was demonstrated to have promising potential to be used for the engineering of large vessel as well as other smooth muscle cell‑containing tissues, including bladder, urethral and intestinal tissues.
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1 

Naito Y, Shinoka T, Duncan D, Hibino N, Solomon D, Cleary M, Rathore A, Fein C, Church S and Breuer C: Vascular tissue engineering: Towards the next generation vascular grafts. Adv Drug Deliv Rev. 63:312–323. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Khait L and Birla RK: Bypassing the patient: Comparison of biocompatible models for the future of vascular tissue engineering. Cell Transplant. 21:269–283. 2012. View Article : Google Scholar

3 

Wang F, Mohammed A, Li C, Ge P, Wang L and King MW: Degradable/non-degradable polymer composites for in-situ tissue engineering small diameter vascular prosthesis application. Biomed Mater Eng. 24:2127–2133. 2014.PubMed/NCBI

4 

Woods I and Flanagan TC: Electrospinning of biomimetic scaffolds for tissue-engineered vascular grafts: Threading the path. Expert Rev Cardiovasc Ther. 12:815–832. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Weinberg CB and Bell E: A blood vessel model constructed from collagen and cultured vascular cells. Science. 231:397–400. 1986. View Article : Google Scholar : PubMed/NCBI

6 

Wang Y, Hu J, Jiao J, Liu Z, Zhou Z, Zhao C, Chang LJ, Chen YE, Ma PX and Yang B: Engineering vascular tissue with functional smooth muscle cells derived from human iPS cells and nanofibrous scaffolds. Biomaterials. 35:8960–8969. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Niklason LE, Gao J, Abbott WM, Hirschi KK, Houser S, Marini R and Langer R: Functional arteries grown in vitro. Science. 284:489–493. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Watanabe M, Shin'oka T, Tohyama S, Hibino N, Konuma T, Matsumura G, Kosaka Y, Ishida T, Imai Y, Yamakawa M, et al: Tissue-engineered vascular autograft: Inferior vena cava replacement in a dog model. Tissue Eng. 7:429–439. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Nerem RM and Seliktar D: Vascular tissue engineering. Ann Rev Biomed Eng. 3:225–243. 2001. View Article : Google Scholar

10 

Galmiche MC, Koteliansky VE, Brière J, Hervé P and Charbord P: Stromal cells from human long-term marrow cultures are mesenchymal cells that differentiate following a vascular smooth muscle differentiation pathway. Blood. 82:66–76. 1993.PubMed/NCBI

11 

Kashiwakura Y, Katoh Y, Tamayose K, Konishi H, Takaya N, Yuhara S, Yamada M, Sugimoto K and Daida H: Isolation of bone marrow stromal cell-derived smooth muscle cells by a human SM22alpha promoter: In vitro differentiation of putative smooth muscle progenitor cells of bone marrow. Circulation. 107:2078–2081. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Shimizu K, Sugiyama S, Aikawa M, Fukumoto Y, Rabkin E, Libby P and Mitchell RN: Host bone-marrow cells are a source of donor intimal smooth-muscle-like cells in murine aortic transplant arteriopathy. Nat Med. 7:738–741. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Gong Z, Calkins G, Cheng EC, Krause D and Niklason LE: Influence of culture medium on smooth muscle cell differentiation from human bone marrow derived mesenchymal stem cells. Tissue Eng Part A. 15:319–330. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Wang N, Ren GD, Zhou Z, Xu Y, Qin T, Yu RF and Zhang TC: Cooperation of myocardin and smad2 in inducing differentiation of mesenchymal stem cells into smooth muscle cells. IUBMB Life. 64:331–339. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Kusuma S, Facklam A and Gerecht S: Characterizing human pluripotent stem cell-derived vascular cells for tissue engineering applications. Stem Cells Dev. 24:451–458. 2015. View Article : Google Scholar

16 

Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues. Science. 276:71–74. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, Gardner R, Neutzel S and Sharkis SJ: Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell. 105:369–377. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Colter DC, Sekiya I and Prockop DJ: Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA. 98:7841–7845. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Fujiwara H, Oda K, Saiki Y, Sakamoto N, Ohashi T, Sato M, Tabata Y and Tabayashi K: The wrapping method using biodegradable felt strips has a preventive effect on the thinning of the aortic wall: Experimental study in the canine aorta. J Vasc Surg. 43:349–356. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Schaner PJ, Martin ND, Tulenko TN, Shapiro IM, Tarola NA, Leichter RF, Carabasi RA and Dimuzio PJ: Decellularized vein as a potential scaffold for vascular tissue engineering. J Vasc Surg. 40:146–153. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Reddy GK and Ewemeka CS: A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 29:225–229. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Cho SW, Kim IK, Lim SH, Kim DI, Kang SW, Kim SH, Kim YH, Lee EY, Choi CY and Kim BS: Smooth muscle-like tissues engineered with bone marrow stromal cells. Biomaterials. 25:2979–2986. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Gong Z and Niklason LE: Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs). FASEB J. 22:1635–1648. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Zhang G, Drinnan CT, Geuss LR and Suggs LJ: Vascular differentiation of bone marrow stem cells is directed by a tunable three-dimensional matrix. Acta Biomater. 6:3395–3403. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Sekiguchi H, Ii M, Jujo K, Yokoyama A, Hagiwara N and Asahara T: Improved culture-based isolation of differentiating endothelial progenitor cells from mouse bone marrow mononuclear cells. PloS One. 6:e286392011. View Article : Google Scholar

27 

van den Akker NM, Kolk FF, Jeukens F, Verbruggen S, Gagliardi M, Dullens S, Heschel I, Post MJ, Molin DG and Waltenberger J: Vascular potency of Sus scrofa bone marrow-derived mesenchymal stem cells: A progenitor source of medial but not endothelial cells. Tissue Eng Part A. 18:828–839. 2012. View Article : Google Scholar

28 

Roh JD, Brennan MP, Lopez-Soler RI, Fong PM, Goyal A, Dardik A and Breuer CK: Construction of an autologous tissue-engineered venous conduit from bone marrow-derived vascular cells: Optimization of cell harvest and seeding techniques. J Pediatr Surg. 42:198–202. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Xu H, Su J, Sun J and Ren T: Preparation and characterization of new nano-composite scaffolds loaded with vascular stents. Int J Mol Sci. 13:3366–3381. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Friedman SG, Lazzaro RS, Spier LN, Moccio C and Tortolani AJ: A prospective randomized comparison of Dacron and polytetrafluoroethylene aortic bifurcation grafts. Surgery. 117:7–10. 1995. View Article : Google Scholar : PubMed/NCBI

31 

Peck M, Gebhart D, Dusserre N, McAllister TN and L'Heureux N: The evolution of vascular tissue engineering and current state of the art. Cells Tissues Organs. 195:144–158. 2012. View Article : Google Scholar :

32 

Pankajakshan D and Agrawal DK: Scaffolds in tissue engineering of blood vessels. Can J Physiol Pharmacol. 88:855–873. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Gui L, Zhao L, Spencer RW, Burghouwt A, Taylor MS, Shalaby SW and Niklason LE: Development of novel biodegradable polymer scaffolds for vascular tissue engineering. Tissue Eng Part A. 17:1191–1200. 2011. View Article : Google Scholar :

34 

Engbers-Buijtenhuijs P, Buttafoco L, Poot AA, Dijkstra PJ, de Vos RA, Sterk LM, Geelkerken RH, Vermes I and Feijen J: Biological characterization of vascular grafts cultured in a bioreactor. Biomaterials. 27:2390–2397. 2006. View Article : Google Scholar

35 

Arrigoni C, Chittò A, Mantero S and Remuzzi A: Rotating versus perfusion bioreactor for the culture of engineered vascular constructs based on hyaluronic acid. Biotechnol Bioeng. 100:988–997. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Song Y, Wennink JW, Kamphuis MM, Sterk LM, Vermes I, Poot AA, Feijen J and Grijpma DW: Dynamic culturing of smooth muscle cells in tubular poly (trimethylene carbonate) scaffolds for vascular tissue engineering. Tissue Eng Part A. 17:381–387. 2011. View Article : Google Scholar

37 

Couet F and Mantovani D: A new bioreactor adapts to materials state and builds a growth model for vascular tissue engineering. Artif Organs. 36:438–445. 2012. View Article : Google Scholar

38 

Seliktar D, Nerem RM and Galis ZS: The role of matrix metalloproteinase-2 in the remodeling of cell-seeded vascular constructs subjected to cyclic strain. Ann Biomed Eng. 29:923–934. 2001. View Article : Google Scholar

39 

Stegemann JP and Nerem RM: Phenotype modulation in vascular tissue engineering using biochemical and mechanical stimulation. Ann Biomed Eng. 31:391–402. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Nikolovski J, Kim BS and Mooney DJ: Cyclic strain inhibits switching of smooth muscle cells to an osteoblast-like phenotype. FASEB J. 17:455–457. 2003.PubMed/NCBI

41 

Kim BS and Mooney DJ: Scaffolds for engineering smooth muscle under cyclic mechanical strain conditions. J Biomech Eng. 122:210–215. 2000. View Article : Google Scholar : PubMed/NCBI

42 

Stegemann JP, Hong H and Nerem RM: Mechanical, biochemical and extracellular matrix effects on vascular smooth muscle cell phenotype. J Appl Physiol (1985). 98:2321–2327. 2005. View Article : Google Scholar

43 

Hamilton DW, Maul TM and Vorp DA: Characterization of the response of bone marrow-derived progenitor cells to cyclic strain: Implications for vascular tissue-engineering applications. Tissue Eng. 10:361–369. 2004. View Article : Google Scholar : PubMed/NCBI

44 

Patel A, Fine B, Sandig M and Mequanint K: Elastin biosynthesis: The missing link in tissue-engineered blood vessels. Cardiovasc Res. 71:40–49. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Opitz F, Schenke-Layland K, Cohnert TU, Starcher B, Halbhuber KJ, Martin DP and Stock UA: Tissue engineering of aortic tissue: Dire consequence of suboptimal elastic fiber synthesis in vivo. Cardiovasc Res. 63:719–730. 2004. View Article : Google Scholar : PubMed/NCBI

46 

Long JL and Tranquillo RT: Elastic fiber production in cardiovascular tissue-equivalents. Matrix Biol. 22:339–350. 2003. View Article : Google Scholar : PubMed/NCBI

47 

Ramamurthi A and Vesely I: Evaluation of the matrix-synthesis potential of crosslinked hyaluronan gels for tissue engineering of aortic heart valves. Biomaterials. 26:999–1010. 2005. View Article : Google Scholar

48 

Thomas LV and Nair PD: The effect of pulsatile loading and scaffold structure for the generation of a medial equivalent tissue engineered vascular graft. Biores Open Access. 2:227–239. 2013. View Article : Google Scholar : PubMed/NCBI

49 

Kanda K, Matsuda T and Oka T: Mechanical stress induced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells. ASAIO J. 39:M686–M690. 1993. View Article : Google Scholar : PubMed/NCBI

50 

Chiquet M, Matthisson M, Koch M, Tannheimer M and Chiquet-Ehrismann R: Regulation of extracellular matrix synthesis by mechanical stress. Biochem Cell Biol. 74:737–744. 1996. View Article : Google Scholar : PubMed/NCBI

51 

Kim BS and Mooney DJ: Scaffols for engineering smooth muscle under cyclic mechanical strain conditions. J Biomech Eng. 122:210–215. 2000. View Article : Google Scholar : PubMed/NCBI

52 

Shi ZD and Tarbell JM: Fluid flow mechanotransduction in vascular smooth muscle cells and fibroblasts. Ann Biomed Eng. 39:1608–1619. 2011. View Article : Google Scholar : PubMed/NCBI

53 

Oluwole BO, Du W, Mills I and Sumpio BE: Gene regulation by mechanical forces. Endothelium. 5:85–93. 1997. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Xu ZC, Zhang Q and Li H: Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor. Mol Med Rep 12: 6005-6012, 2015.
APA
Xu, Z.C., Zhang, Q., & Li, H. (2015). Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor. Molecular Medicine Reports, 12, 6005-6012. https://doi.org/10.3892/mmr.2015.4147
MLA
Xu, Z. C., Zhang, Q., Li, H."Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor". Molecular Medicine Reports 12.4 (2015): 6005-6012.
Chicago
Xu, Z. C., Zhang, Q., Li, H."Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor". Molecular Medicine Reports 12, no. 4 (2015): 6005-6012. https://doi.org/10.3892/mmr.2015.4147
Copy and paste a formatted citation
x
Spandidos Publications style
Xu ZC, Zhang Q and Li H: Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor. Mol Med Rep 12: 6005-6012, 2015.
APA
Xu, Z.C., Zhang, Q., & Li, H. (2015). Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor. Molecular Medicine Reports, 12, 6005-6012. https://doi.org/10.3892/mmr.2015.4147
MLA
Xu, Z. C., Zhang, Q., Li, H."Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor". Molecular Medicine Reports 12.4 (2015): 6005-6012.
Chicago
Xu, Z. C., Zhang, Q., Li, H."Elastic large muscular vessel wall engineered with bone marrow‑derived cells under pulsatile stimulation in a bioreactor". Molecular Medicine Reports 12, no. 4 (2015): 6005-6012. https://doi.org/10.3892/mmr.2015.4147
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