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Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis

  • Authors:
    • Jie Du
    • Chunyou Chen
    • Yiqun Sun
    • Lin Zheng
    • Wanchen Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Port Hospital of Tianjin, Tianjin 300456, P.R. China
    Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5816-5820
    |
    Published online on: July 29, 2015
       https://doi.org/10.3892/mmr.2015.4150
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Abstract

Ponicidin is a diterpenoid extracted from the Chinese herb Isodon adenolomus, which has been reported as a therapeutic cytotoxic drug that may be used to treat various types of human cancer. The present study aimed to determine the antitumor effects of ponicidin, and to investigate its underlying mechanisms in colorectal cancer. The HT29 colorectal cancer cell line was used to detect the cytotoxicity of various doses of ponicidin. Cell proliferation was measured using a Cell Counting kit‑8 assay. Cell cycle and apoptosis analyses were performed using flow cytometry and fluorescent microscopy. Western blot analysis was used to measure the expression levels of apoptosis‑associated proteins following treatment with ponicidin. Treatment with ponicidin significantly suppressed HT29 cell growth by inducing G1 cell cycle arrest and apoptosis. The AKT and MEK signaling pathways were also suppressed by ponicidin; however, the p38 signaling pathway was significantly activated. The expression levels of caspase 3 and Bax protein were markedly upregulated following treatment with ponicidin. These results suggest that ponicidin exerts significant antitumor effects via the induction of cell cycle arrest and apoptosis in colorectal cells. In conclusion, ponicidin acted as an inducer of apoptosis, and may be used as a therapeutic cytotoxic drug to treat human cancer, including colorectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Du J, Chen C, Sun Y, Zheng L and Wang W: Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis. Mol Med Rep 12: 5816-5820, 2015.
APA
Du, J., Chen, C., Sun, Y., Zheng, L., & Wang, W. (2015). Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis. Molecular Medicine Reports, 12, 5816-5820. https://doi.org/10.3892/mmr.2015.4150
MLA
Du, J., Chen, C., Sun, Y., Zheng, L., Wang, W."Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis". Molecular Medicine Reports 12.4 (2015): 5816-5820.
Chicago
Du, J., Chen, C., Sun, Y., Zheng, L., Wang, W."Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis". Molecular Medicine Reports 12, no. 4 (2015): 5816-5820. https://doi.org/10.3892/mmr.2015.4150
Copy and paste a formatted citation
x
Spandidos Publications style
Du J, Chen C, Sun Y, Zheng L and Wang W: Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis. Mol Med Rep 12: 5816-5820, 2015.
APA
Du, J., Chen, C., Sun, Y., Zheng, L., & Wang, W. (2015). Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis. Molecular Medicine Reports, 12, 5816-5820. https://doi.org/10.3892/mmr.2015.4150
MLA
Du, J., Chen, C., Sun, Y., Zheng, L., Wang, W."Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis". Molecular Medicine Reports 12.4 (2015): 5816-5820.
Chicago
Du, J., Chen, C., Sun, Y., Zheng, L., Wang, W."Ponicidin suppresses HT29 cell growth via the induction of G1 cell cycle arrest and apoptosis". Molecular Medicine Reports 12, no. 4 (2015): 5816-5820. https://doi.org/10.3892/mmr.2015.4150
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