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Article

Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia

  • Authors:
    • Xuerong Li
    • Xiaodan Liu
    • Yanxia Zhao
    • Ren Zhong
    • Aiqin Song
    • Lirong Sun
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Hematology and Oncology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
  • Pages: 6093-6097
    |
    Published online on: July 30, 2015
       https://doi.org/10.3892/mmr.2015.4153
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Abstract

To determine the effect of thymosin α1 (Tα1) on the phenotypic and functional maturation of HL‑60 cells, freeze‑thaw antigen‑loaded dendritic cells (DCs) were derived from peripheral blood mononuclear cells (PBMCs) of children with acute lymphoblastic leukemia (ALL). The DCs were generated from the PBMC samples that were collected from the PB of 10 consecutive ALL children. On day 3 of culturing, the cells in the antigen + no Tα1 (AN) and antigen + Tα1 (AT) groups were incubated with 100 µl lysates obtained from freeze‑thaw cycling. After 5 days of incubation, the AT group was administered with 100 ng/ml Tα1. On day 8, the DCs were stained with fluorescein isothiocyanate‑conjugated cluster of differentiation (CD)1a, CD83 and HLA‑DR antibodies and analyzed by flow cytometry. In addition, the killing activity of cytotoxic T lymphocytes (CTLs) from the different groups on wild‑type leukemia cells was measured. The DCs in the AT group exhibited more apparent, characteristic dendritic morphologies than the control and AN group DCs. Furthermore, the lowest expression level of CD1a, and the highest expression of CD83 and HLA‑DR were observed in the AT group when compared with the AN and control groups (P<0.05). The lactate dehydrogenase release assay demonstrated that the killing rate of CTL in the AT group was significantly higher than that in the control and AN groups (P<0.01). Thus, Tα1 may markedly promote the phenotypic and functional maturation of DCs, and may serve as a suitable immunomodulator of DC‑based immunotherapy for treatment of hematological malignancies.
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View References

1 

Essig S, Li Q, Chen Y, Hitzler J, Leisenring W, Greenberg M, Sklar C, Hudson MM, Armstrong GT, Krull KR, et al: Risk of late effects of treatment in children newly diagnosed with standard-risk acute lymphoblastic leukaemia: A report from the Childhood Cancer Survivor Study cohort. Lancet Oncol. 15:841–851. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Turcotte LM and Neglia JP: Survivors of childhood cancer: Risk of new primary neoplasms of the CNS. Tumors of the Central Nervous System. Hayat MA: ISSN: 2215-096X12. Springer; Dordrecht: pp. 137–pp145. 2014, View Article : Google Scholar

3 

McCarthy PL, Owzar K and Hahn T: Autologous hematopoietic stem cell transplantation and maintenance therapy for multiple myeloma. Int J Hematol Oncol. 2:71–83. 2013. View Article : Google Scholar

4 

Rein LA, Sung AD and Rizzieri DA: New approaches to manipulate minimal residual disease after allogeneic stem cell transplantation. Int J Hematol Oncol. 2:39–48. 2013. View Article : Google Scholar

5 

Duncan C and Roddie H: Dendritic cell vaccines in acute leukaemia. Best Pract Res Clin Haematol. 21:521–541. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Conrad DP, Tsang J, Maclean M, Diallo JS, Le Boeuf F, Lemay CG, Falls TJ and Parato KA: Leukemia cell-rhabdovirus vaccine: Personalized immunotherapy for acute lymphoblastic leukemia. Clin Cancer Res. 19:3832–3843. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Pospísilová D, Borovicková J, Poloucková A, Spísek R, Sedivá A, Hrusák O, Starý J and Bartůnková J: Generation of functional dendritic cells for potential use in the treatment of acute lympho-blastic leukemia. Cancer Immunol Immunother. 51:72–78. 2002. View Article : Google Scholar

8 

Maggio R, Peragine N, Calabrese E, De Propris MS, Intoppa S, Della Starza I, Ariola C, Vitale A, Foà R and Guarini A: Generation of functional dendritic cells (DC) in adult acute lymphoblastic leukemia: Rationale for a DC-based vaccination program for patients in complete hematological remission. Leuk Lymphoma. 48:302–310. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Malissen B, Tamoutounour S and Henri S: The origins and functions of dendritic cells and macrophages in the skin. Nat Rev Immunol. 14:417–428. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Ma Y, Aymeric L, Locher C, Kroemer G and Zitvogel L: The dendritic cell-tumor cross-talk in cancer. Curr Opin Immunol. 23:146–152. 2011. View Article : Google Scholar

11 

Palucka K and Banchereau J: Cancer immunotherapy via dendritic cells. Nat Rev Cancer. 12:265–277. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Subklewe M, Geiger C, Lichtenegger FS, Javorovic M, Kvalheim G, Schendel DJ and Bigalke I: New generation dendritic cell vaccine for immunotherapy of acute myeloid leukemia. Cancer Immunol Immunother. 63:1093–1103. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Anton D, Dabadghao S, Palucka K, Holm G and Yi Q: Generation of dendritic cells from peripheral blood adherent cells in medium with human serum. Scand J Immunol. 47:116–121. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Jeras M, Bergant M and Repnik U: In vitro preparation and functional assessment of human monocyte-derived dendritic cells-potential antigen-specific modulators of in vivo immune responses. Transpl Immunol. 14:231–244. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Bach JF: Thymic hormones. J Immunopharmacol. 1:277–310. 1979. View Article : Google Scholar : PubMed/NCBI

16 

Goldstein AL, Guha A, Zatz MM, Hardy MA and White A: Purification and biological activity of thymosin, a hormone of the thymus gland. Proc Natl Acad Sci USA. 69:1800–1803. 1972. View Article : Google Scholar : PubMed/NCBI

17 

Romani L, Bistoni F, Montagnoli C, Gaziano R, Bozza S, Bonifazi P, Zelante T, Moretti S, Rasi G, Garaci E, et al: Thymosin alpha1: An endogenous regulator of inflammation, immunity, and tolerance. Ann N Y Acad Sci. 1112:326–338. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Fan Y, Chang H, Yu Y, Liu J and Wang R: Thymosin α 1 suppresses proliferation and induces apoptosis in human leukemia cell lines. Peptides. 27:2165–2173. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Lin JJ, Hsu HY, Yang JS, Lu KW, Wu RS, Wu KC, Lai TY, Chen PY, Ma CY, Wood WG, et al: Molecular evidence of anti-leukemia activity of gypenosides on human myeloid leukemia HL-60 cells in vitro and in vivo using a HL-60 cells murine xenograft model. Phytomedicine. 18:1075–1085. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Fidan I, Yesilyurt E, Kalkanci A, Aslan SO, Sahin N, Ogan MC and Dizbay M: Immunomodulatory effects of voriconazole and caspofungin on human peripheral blood mononuclear cells stimulated by Candida albicans and Candida krusei. Am J Med Sci. 348:219–223. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Yao Q, Doan LX, Zhang R, Bharadwaj U, Li M and Chen C: Thymosin-alpha1 modulates dendritic cell differentiation and functional maturation from human peripheral blood CD14+ monocytes. Immunol Lett. 110:110–120. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Eisen S, Wedner H and Parker C: Isolation of pure human pexipherac blood T-lymphocytes using nylon wool columns. Immunol Invest. 1:571–577. 1972. View Article : Google Scholar

23 

Timmerman JM and Levy R: Dendritic cell vaccines for cancer immunotherapy. Annu Rev Med. 50:507–529. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Banchereau J and Palucka AK: Dendritic cells as therapeutic vaccines against cancer. Nat Rev Immunol. 5:296–306. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Sabado RL, Miller E, Spadaccia M, Vengco I, Hasan F and Bhardwaj N: Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy. J Vis Exp. View Article : Google Scholar : 2013.PubMed/NCBI

26 

Huang Y, Chen Z, Zhou C, Yao H, Li M and Xu C: The modulation of thymosin alpha 1 in the maturation, differentiation and function of murine bone marrow-derived dendritic cells in the absence or presence of tumor necrosis factor-alpha. Int Immunopharmacol. 4:539–546. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Ragde H, Cavanagh WA and Tjoa BA: Dendritic cell based vaccines: Progress in immunotherapy studies for prostate cancer. J Urol. 172:2532–2538. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Liu S, Yu Y, Zhang M, Wang W and Cao X: The involvement of TNF-alpha-related apoptosis-inducing ligand in the enhanced cytotoxicity of IFN-beta-stimulated human dendritic cells to tumor cells. J Immunol. 166:5407–5415. 2001. View Article : Google Scholar : PubMed/NCBI

29 

MartIn-Fontecha A, Sebastiani S, Höpken UE, Uguccioni M, Lipp M, Lanzavecchia A and Sallusto F: Regulation of dendritic cell migration to the draining lymph node: Impact on T lymphocyte traffic and priming. J Exp Med. 198:615–621. 2003. View Article : Google Scholar : PubMed/NCBI

30 

de Vries IJ, Lesterhuis WJ, Scharenborg NM, Engelen LP, Ruiter DJ, Gerritsen MJ, Croockewit S, Britten CM, Torensma R, Adema GJ, et al: Maturation of dendritic cells is a prerequisite for inducing immune responses in advanced melanoma patients. Clin Cancer Res. 9:5091–5100. 2003.PubMed/NCBI

31 

León B and Ardavín C: Monocyte-derived dendritic cells in innate and adaptive immunity. Immunol Cell Biol. 86:320–324. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Slukvin II, Thomson JA, Vodyanyk MA and Gumenyuk ME: Method of forming dendritic cells from embryonic stem cells. US Patent no 8,785,189. Washington, DC: U.S. Patent and Trademark Office; 2014, Filed Feb 1, 2012; issued May 7, 2013.

33 

Cao X, Sugita M, van der Wel N, Lai J, Rogers RA, Peters PJ and Brenner MB: CD1 molecules efficiently present antigen in immature dendritic cells and traffic independently of MHC class II during dendritic cell maturation. J Immunol. 169:4770–4777. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Tan SM, Kapp M, Flechsig C, Kapp K, Rachor JE, Eyrich M, Loeffler J, Einsele H and Grigoleit GU: Stimulating surface molecules, Th1-polarizing cytokines, proven trafficking-a new protocol for the generation of clinical-grade dendritic cells. Cytotherapy. 15:492–506. 2013. View Article : Google Scholar : PubMed/NCBI

35 

van der Wel NN, Sugita M, Fluitsma DM, Cao X, Schreibelt G, Brenner MB and Peters PJ: CD1 and major histocompatibility complex II molecules follow a different course during dendritic cell maturation. Mol Biol Cell. 14:3378–3388. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Lechmann M, Berchtold S, Steinkasserer A and Hauber J: CD83 on dendritic cells: More than just a marker for maturation. Trends Immunol. 23:273–275. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Prechtel AT, Turza NM, Theodoridis AA and Steinkasserer A: CD83 knockdown in monocyte-derived dendritic cells by small interfering RNA leads to a diminished T cell stimulation. J Immunol. 178:5454–5464. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Drénou B, Amiot L, Setterblad N, Taque S, Guilloux V, Charron D, Fauchet R and Mooney N: MHC class II signaling function is regulated during maturation of plasmacytoid dendritic cells. J Leukoc Biol. 77:560–567. 2005. View Article : Google Scholar : PubMed/NCBI

39 

Li XL, Zhao YX, Sun LR, Yang J and Xu HJ: The preparation of HL-60 cells vaccine expressing BCG heat shock protein 70 and detection of its immunogenicity in vitro. Hum Vaccin Immunother. 8:1376–1381. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li X, Liu X, Zhao Y, Zhong R, Song A and Sun L: Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia. Mol Med Rep 12: 6093-6097, 2015.
APA
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., & Sun, L. (2015). Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia. Molecular Medicine Reports, 12, 6093-6097. https://doi.org/10.3892/mmr.2015.4153
MLA
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., Sun, L."Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia". Molecular Medicine Reports 12.4 (2015): 6093-6097.
Chicago
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., Sun, L."Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia". Molecular Medicine Reports 12, no. 4 (2015): 6093-6097. https://doi.org/10.3892/mmr.2015.4153
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Liu X, Zhao Y, Zhong R, Song A and Sun L: Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia. Mol Med Rep 12: 6093-6097, 2015.
APA
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., & Sun, L. (2015). Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia. Molecular Medicine Reports, 12, 6093-6097. https://doi.org/10.3892/mmr.2015.4153
MLA
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., Sun, L."Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia". Molecular Medicine Reports 12.4 (2015): 6093-6097.
Chicago
Li, X., Liu, X., Zhao, Y., Zhong, R., Song, A., Sun, L."Effect of thymosin α1 on the phenotypic and functional maturation of dendritic cells from children with acute lymphoblastic leukemia". Molecular Medicine Reports 12, no. 4 (2015): 6093-6097. https://doi.org/10.3892/mmr.2015.4153
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