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Article

Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways

  • Authors:
    • Tsai‑Kun Wu
    • Chyou‑Wei Wei
    • Ying‑Ru Pan
    • Shur‑Hueih Cherng
    • Wei‑Jung Chang
    • Hsueh‑Fang Wang
    • Yung‑Luen Yu
  • View Affiliations / Copyright

    Affiliations: The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 404, Taiwan, R.O.C., Deparment of Nutrition, Hungkuang University, Taichung 433, Taiwan, R.O.C., Deparment of Biotechnology, Hungkuang University, Taichung 433, Taiwan, R.O.C., Graduate Institute of Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
  • Pages: 6086-6092
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    Published online on: August 3, 2015
       https://doi.org/10.3892/mmr.2015.4167
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Abstract

Aristolochic acid (AA) is a component of Chinese medicinal herbs, including asarum and aristolochia and has been used in Traditional Chinese Medicine for a long time. Recent studies found that AA has a cytotoxic effect resulting in nephropathy. These studies indicated that AA‑induced cytotoxicity is associated with increases in oxidative stress and caspase‑3 activation. The present study further demonstrated that AA mainly elevates the H2O2 ratio, leading to increases in oxidative stress. Furthermore, the results indicated that AA induces cell death can via caspase‑dependent and ‑independent pathways. It is desirable to identify means of inhibiting AA‑induced renal damage; therefore, the present study applied an anti‑oxidative nutrient, vitamin C, to test whether it can be employed to reduce AA‑induced cell cytotoxicity. The results showed that vitamin C decreased AA‑induced H2O2 levels, caspase‑3 activity and cytotoxicity in renal tubular cells. In conclusion, the present study was the first to demonstrate that AA‑induced increases of the H2O2 ratio resulted in renal tubular cell death via caspase‑dependent and ‑independent pathways, and that vitamin C can decrease AA‑induced increases in H2O2 levels and caspase‑3 activity to attenuate AA‑induced cell cytotoxicity.
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Copy and paste a formatted citation
Spandidos Publications style
Wu TK, Wei CW, Pan YR, Cherng SH, Chang WJ, Wang HF and Yu YL: Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways. Mol Med Rep 12: 6086-6092, 2015.
APA
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., & Yu, Y. (2015). Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways. Molecular Medicine Reports, 12, 6086-6092. https://doi.org/10.3892/mmr.2015.4167
MLA
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., Yu, Y."Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways". Molecular Medicine Reports 12.4 (2015): 6086-6092.
Chicago
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., Yu, Y."Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways". Molecular Medicine Reports 12, no. 4 (2015): 6086-6092. https://doi.org/10.3892/mmr.2015.4167
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Spandidos Publications style
Wu TK, Wei CW, Pan YR, Cherng SH, Chang WJ, Wang HF and Yu YL: Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways. Mol Med Rep 12: 6086-6092, 2015.
APA
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., & Yu, Y. (2015). Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways. Molecular Medicine Reports, 12, 6086-6092. https://doi.org/10.3892/mmr.2015.4167
MLA
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., Yu, Y."Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways". Molecular Medicine Reports 12.4 (2015): 6086-6092.
Chicago
Wu, T., Wei, C., Pan, Y., Cherng, S., Chang, W., Wang, H., Yu, Y."Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress‑mediated cell death pathways". Molecular Medicine Reports 12, no. 4 (2015): 6086-6092. https://doi.org/10.3892/mmr.2015.4167
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