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Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis

  • Authors:
    • Kuan Zeng
    • Bao Ping Deng
    • Hui‑Qi Jiang
    • Meng Wang
    • Ping Hua
    • Hong‑Wu Zhang
    • Yu‑Bin Deng
    • Yan‑Qi Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China, Research Center of Translational Medicine, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5723-5729
    |
    Published online on: August 5, 2015
       https://doi.org/10.3892/mmr.2015.4176
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Abstract

Mesenchymal stem cells (MSCs) have become a recent focus of experimental and clinical research regarding myocardial regeneration. However, the therapeutic potential of these cells is limited by poor survival. Prostaglandin E1 (PGE1) is known to have anti‑inflammatory and anti‑apoptotic effects on the myocardium. The aim of the present study was to determine whether PGE1 could protect MSCs against serum deprivation (SD)‑induced apoptosis. An SD model was used to induce apoptosis in MSCs in vitro. Apoptotic morphological changes were detected by Hoechst 33258 fluorescent nuclear staining; and Annexin V‑fluorescein isothiocyanate/propidium iodide (PI) double staining and flow cytometry was used to quantify the rate of apoptosis. Western blot analysis was used to detect the expression levels of the apoptosis‑associated proteins Bcl‑2, Bax and caspase‑3. The results of the present study demonstrated that SD induced apoptosis of MSCs, and that treatment with PGE1 attenuated the morphological changes characteristic of apoptosis. Annexin V/PI staining showed that the rate of apoptosis gradually increased with the duration of ischemia. Furthermore, treatment with PGE1 significantly reduced SD‑induced apoptosis, decreased the protein expression levels of Bax and caspase‑3, and increased the expression levels of Bcl‑2. These data suggest that PGE1 is able to influence the survival of MSCs under certain conditions. These results may aid in improving the therapeutic efficacy of MSC transplantation used to treat chronic ischemic heart disease.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng K, Deng BP, Jiang HQ, Wang M, Hua P, Zhang HW, Deng YB and Yang YQ: Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis. Mol Med Rep 12: 5723-5729, 2015.
APA
Zeng, K., Deng, B.P., Jiang, H., Wang, M., Hua, P., Zhang, H. ... Yang, Y. (2015). Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis. Molecular Medicine Reports, 12, 5723-5729. https://doi.org/10.3892/mmr.2015.4176
MLA
Zeng, K., Deng, B. P., Jiang, H., Wang, M., Hua, P., Zhang, H., Deng, Y., Yang, Y."Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis". Molecular Medicine Reports 12.4 (2015): 5723-5729.
Chicago
Zeng, K., Deng, B. P., Jiang, H., Wang, M., Hua, P., Zhang, H., Deng, Y., Yang, Y."Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis". Molecular Medicine Reports 12, no. 4 (2015): 5723-5729. https://doi.org/10.3892/mmr.2015.4176
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng K, Deng BP, Jiang HQ, Wang M, Hua P, Zhang HW, Deng YB and Yang YQ: Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis. Mol Med Rep 12: 5723-5729, 2015.
APA
Zeng, K., Deng, B.P., Jiang, H., Wang, M., Hua, P., Zhang, H. ... Yang, Y. (2015). Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis. Molecular Medicine Reports, 12, 5723-5729. https://doi.org/10.3892/mmr.2015.4176
MLA
Zeng, K., Deng, B. P., Jiang, H., Wang, M., Hua, P., Zhang, H., Deng, Y., Yang, Y."Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis". Molecular Medicine Reports 12.4 (2015): 5723-5729.
Chicago
Zeng, K., Deng, B. P., Jiang, H., Wang, M., Hua, P., Zhang, H., Deng, Y., Yang, Y."Prostaglandin E1 protects bone marrow‑derived mesenchymal stem cells against serum deprivation‑induced apoptosis". Molecular Medicine Reports 12, no. 4 (2015): 5723-5729. https://doi.org/10.3892/mmr.2015.4176
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