Tectonic 1 accelerates gastric cancer cell proliferation and cell cycle progression in vitro

Wang,Xinbao; Yu,Qiming; Zhang,Yingli; Ling,Zhiqiang; Yu,Pengfei

doi:10.3892/mmr.2015.4177

October-2015 Volume 12 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

October-2015 Volume 12 Issue 4

Full Size Image

Article

Tectonic 1 accelerates gastric cancer cell proliferation and cell cycle progression in vitro

Authors:
- Xinbao Wang
- Qiming Yu
- Yingli Zhang
- Zhiqiang Ling
- Pengfei Yu
View Affiliations / Copyright

Affiliations: Department of Abdominal Tumor Surgery, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China, Department of Clinic, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China, Department of Cancer Research Institute, Zhejiang Cancer Hospital, Zhejiang Cancer Center, Banshanqiao, Hangzhou 310022, P.R. China
Pages: 5897-5902
|
Published online on: August 5, 2015

https://doi.org/10.3892/mmr.2015.4177
Expand metrics +

Abstract

Hedgehog (Hh) pathway is important in development and cancer. Hh signaling is constitutively active in gastric cancer. Recently, tectonic 1 (TCTN1) was identified as one regulator of the Hh pathway. In the present study, the biological role of TCTN1 was examined in gastric cancer via an RNA interference lentivirus system. The constructed lentivirus efficiently suppressed TCTN1 expression in three gastric cancer cell lines. The proliferation of gastric cancer cells was significantly inhibited in TCTN1 knockdown cells, as determined by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide and colony formation assays. Furthermore, in order to determine the underlying mechanism, the cell cycle progression of MGC80‑3 cells was analyzed by flow cytometry. Knockdown of TCTN1 led to cell cycle arrest at the G2/M phase, which contributed to inhibition of growth. In conclusion, the results demonstrated that TCTN1 was essential in the growth of gastric cancer cells in vitro, suggesting TCTN1 as a potential target candidate for the treatment of gastric cancer.

View Figures

View References

1	Wu WK, Cho CH, Lee CW, et al: Dysregulation of cellular signaling in gastric cancer. Cancer Lett. 295:144–153. 2010. View Article : Google Scholar : PubMed/NCBI
2	Danaei G, Vander Hoorn S, Lopez AD, Murray CJ and Ezzati M: Comparative risk assessment collaborating group (Cancers): Causes of cancer in the world: comparative risk assessment of nine behavioural and environmental risk factors. Lancet. 366:1784–1793. 2005. View Article : Google Scholar : PubMed/NCBI
3	Ramalho-Santos M, Melton DA and McMahon AP: Hedgehog signals regulate multiple aspects of gastrointestinal development. Development. 127:2763–2772. 2000.PubMed/NCBI
4	Dessaud E, McMahon AP and Briscoe J: Pattern formation in the vertebrate neural tube: a sonic hedgehog morphogen-regulated transcriptional network. Development. 135:2489–2503. 2008. View Article : Google Scholar : PubMed/NCBI
5	Chiang C, Litingtung Y, Lee E, et al: Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function. Nature. 383:407–413. 1996. View Article : Google Scholar : PubMed/NCBI
6	Yang L, Xie G, Fan Q and Xie J: Activation of the hedgehog-signaling pathway in human cancer and the clinical implications. Oncogene. 29:469–481. 2010. View Article : Google Scholar
7	Varjosalo M and Taipale J: Hedgehog: functions and mechanisms. Genes Dev. 22:2454–2472. 2008. View Article : Google Scholar : PubMed/NCBI
8	Saqui-Salces M and Merchant JL: Hedgehog signaling and gastrointestinal cancer. Biochim Biophys Acta. 1803:786–795. 2010. View Article : Google Scholar : PubMed/NCBI
9	Kelleher FC: Hedgehog signaling and therapeutics in pancreatic cancer. Carcinogenesis. 32:445–451. 2011. View Article : Google Scholar
10	Shaw G, Price AM, Ktori E, et al: Hedgehog signalling in androgen independent prostate cancer. Eur Urol. 54:1333–1343. 2008. View Article : Google Scholar : PubMed/NCBI
11	Hui M, Cazet A, Nair R, Watkins DN, O'Toole SA and Swarbrick A: The Hedgehog signalling pathway in breast development, carcinogenesis and cancer therapy. Breast Cancer Res. 15:2032013. View Article : Google Scholar : PubMed/NCBI
12	Saran A: Basal cell carcinoma and the carcinogenic role of aberrant Hedgehog signaling. Future Oncol. 6:1003–1014. 2010. View Article : Google Scholar : PubMed/NCBI
13	Watkins DN, Berman DM and Baylin SB: Hedgehog signaling: progenitor phenotype in small-cell lung cancer. Cell Cycle. 2:196–198. 2003. View Article : Google Scholar : PubMed/NCBI
14	Fu H, Hu Z, Wen J, Wang K and Liu Y: TGF-beta promotes invasion and metastasis of gastric cancer cells by increasing fascin1 expression via ERK and JNK signal pathways. Acta Biochim Biophys Sin (Shanghai). 41:648–656. 2009. View Article : Google Scholar
15	Feng R, Xiao C and Zavros Y: The role of Sonic Hedgehog as a regulator of gastric function and differentiation. Vitam Horm. 88:473–489. 2012. View Article : Google Scholar : PubMed/NCBI
16	Wang XD, Inzunza H, Chang H, et al: Mutations in the hedgehog pathway genes SMO and PTCH1 in human gastric tumors. PLoS ONE. 8:e544152013. View Article : Google Scholar : PubMed/NCBI
17	Sun HJ, Liu YJ, Li N, et al: Sublocalization of Rab23, a mediator of Sonic hedgehog signaling pathway, in hepatocellular carcinoma cell lines. Mol Med Rep. 6:1276–1280. 2012.PubMed/NCBI
18	Hou Q, Wu YH, Grabsch H, et al: Integrative genomics identifies RAB23 as an invasion mediator gene in diffuse-type gastric cancer. Cancer Res. 68:4623–4630. 2008. View Article : Google Scholar : PubMed/NCBI
19	Katoh Y and Katoh M: Hedgehog signaling pathway and gastrointestinal stem cell signaling network (review). Int J Mol Med. 18:1019–1023. 2006.Review. PubMed/NCBI
20	Reiter JF and Skarnes WC: Tectonic, a novel regulator of the Hedgehog pathway required for both activation and inhibition. Genes Dev. 20:22–27. 2006. View Article : Google Scholar :
21	Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS, et al: A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Nat Genet. 43:776–784. 2011. View Article : Google Scholar : PubMed/NCBI
22	Alazami AM, Alshammari MJ, Salih MA, et al: Molecular characterization of Joubert syndrome in Saudi Arabia. Hum Mutat. 33:1423–1428. 2012. View Article : Google Scholar : PubMed/NCBI
23	Tiscornia G, Singer O and Verma IM: Production and purification of lentiviral vectors. Nat Protoc. 1:241–245. 2006. View Article : Google Scholar
24	Sakoda T, Kasahara N, Hamamori Y and Kedes L: A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J Mol Cell Cardiol. 31:2037–2047. 1999. View Article : Google Scholar : PubMed/NCBI
25	Chen X, Horiuchi A, Kikuchi N, et al: Hedgehog signal pathway is activated in ovarian carcinomas, correlating with cell proliferation: it's inhibition leads to growth suppression and apoptosis. Cancer Sci. 98:68–76. 2007. View Article : Google Scholar
26	Feng YZ, Shiozawa T, Miyamoto T, et al: Overexpression of hedgehog signaling molecules and its involvement in the proliferation of endometrial carcinoma cells. Clin Cancer Res. 13:1389–1398. 2007. View Article : Google Scholar : PubMed/NCBI
27	Zaidi AH, Komatsu Y, Kelly LA, et al: Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells. Cancer Invest. 31:480–489. 2013. View Article : Google Scholar : PubMed/NCBI
28	McManus MT SP: Gene silencing in mammals by small interfering RNAs. Nat Rev Genet. 3:737–747. 2002. View Article : Google Scholar : PubMed/NCBI
29	Devi G: siRNA-based approaches in cancer therapy. Cancer Gene Ther. 13:819–829. 2006. View Article : Google Scholar : PubMed/NCBI
30	Hou MF, Kuo HC, Li JH, et al: Orai1/CRACM1 overexpression suppresses cell proliferation via attenuation of the store-operated calcium influx-mediated signalling pathway in A549 lung cancer cells. Biochim Biophys Acta. 1810:1278–1284. 2011. View Article : Google Scholar : PubMed/NCBI
31	Yang IH, Tsai YT, Chiu SJ, et al: Involvement of STIM1 and Orai1 in EGF-mediated cell growth in retinal pigment epithelial cells. J Biomed Sci. 20:412013. View Article : Google Scholar : PubMed/NCBI