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October-2015 Volume 12 Issue 4

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Article

MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1

  • Authors:
    • Yudong Zhang
    • Shenghua Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 6178-6184
    |
    Published online on: August 5, 2015
       https://doi.org/10.3892/mmr.2015.4183
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Abstract

Secreted Slit glycoproteins and their Roundabout (Robo) receptors have been identified as important axon guidance molecules. The pivotal role of Slit‑Robo signaling is in regulating cell proliferation. MicroRNAs (miRNAs), a class of small non‑coding RNAs, function as critical regulators of gene expression by binding to the 3'‑untranslated region of mRNAs and causing mRNA degradation or translational repression. The present study demonstrated that downregulation of Robo1 using small interfering RNA inhibited mesenchymal stem cell (MSC) proliferation. Additionally, four miRNAs (miR), including miR‑218, miR‑29a, miR‑146 and miR‑148, inhibited the protein expression of Robo1 in the MSCs, with miR‑29 having the most marked effect. A luciferase reporter assay identified Robo1 as a novel target of miR‑29a. Overexpression of miR‑29a suppressed the protein expression levels of Robo1 and Slit2 and inhibited the viability and proliferation of the MSCs. By contrast, overexpression of Robo1 partly rescued these inhibitory effects of miR‑29a on the MSCs confirming that miR‑29a inhibited MSC viability and proliferation, at least partially, by directly targeting Robo1. These results indicated that the miR‑29a/Robo1 axis is crucial for the regulation of MSC viability and proliferation, suggesting that miR‑29a may serve as a potential clinical target for MSC expansion and stem cell transplantation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y and Zhou S: MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1. Mol Med Rep 12: 6178-6184, 2015.
APA
Zhang, Y., & Zhou, S. (2015). MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1. Molecular Medicine Reports, 12, 6178-6184. https://doi.org/10.3892/mmr.2015.4183
MLA
Zhang, Y., Zhou, S."MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1". Molecular Medicine Reports 12.4 (2015): 6178-6184.
Chicago
Zhang, Y., Zhou, S."MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1". Molecular Medicine Reports 12, no. 4 (2015): 6178-6184. https://doi.org/10.3892/mmr.2015.4183
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y and Zhou S: MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1. Mol Med Rep 12: 6178-6184, 2015.
APA
Zhang, Y., & Zhou, S. (2015). MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1. Molecular Medicine Reports, 12, 6178-6184. https://doi.org/10.3892/mmr.2015.4183
MLA
Zhang, Y., Zhou, S."MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1". Molecular Medicine Reports 12.4 (2015): 6178-6184.
Chicago
Zhang, Y., Zhou, S."MicroRNA‑29a inhibits mesenchymal stem cell viability and proliferation by targeting Roundabout 1". Molecular Medicine Reports 12, no. 4 (2015): 6178-6184. https://doi.org/10.3892/mmr.2015.4183
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