Lentivirus‑delivered nemo‑like kinase small interfering RNA inhibits laryngeal cancer cell proliferation in vitro

Tai,Jun; Rao,Yuansheng; Fang,Jugao; Huang,Zhigang; Yu,Zhenkun; Chen,Xiaohong; Zhou,Weiguo; Xiao,Xiao; Long,Ting; Han,Yang; Liu,Qiaoyin; Li,Aidong; Ni,Xin

doi:10.3892/mmr.2015.4189

October-2015 Volume 12 Issue 4

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October-2015 Volume 12 Issue 4

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Article Open Access

Lentivirus‑delivered nemo‑like kinase small interfering RNA inhibits laryngeal cancer cell proliferation in vitro

Authors:
- Jun Tai
- Yuansheng Rao
- Jugao Fang
- Zhigang Huang
- Zhenkun Yu
- Xiaohong Chen
- Weiguo Zhou
- Xiao Xiao
- Ting Long
- Yang Han
- Qiaoyin Liu
- Aidong Li
- Xin Ni
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Affiliations: Beijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China, Department of Otolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China, Key Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China, Department of Center Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, P.R. China, Department of Otolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China

Copyright: © Tai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5619-5624
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Published online on: August 6, 2015

https://doi.org/10.3892/mmr.2015.4189
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Abstract

Laryngeal squamous cell carcinoma is the most common form of head and neck squamous cell carcinoma. Multiple approaches have been applied to treat this type of cancer; however, no significant improvement in survival rate has been achieved. In the present study, the role of nemo‑like kinase (NLK) in human laryngeal carcinoma Hep‑2 cells was investigated. NLK has been identiﬁed as an important regulator of cell growth, patterning and cell death in a variety of organisms. Lentivirus‑mediated‑shRNA was employed to silence endogenous NLK expression. Downregulation of the expression of NLK following lentivirus infection was confirmed using reverse transcription quantitative polymerase chain reaction and western blot analysis. The effects of NLK downregulation on Hep‑2 cell proliferation and cell cycle progression were analyzed using an MTT assay and flow cytometry, respectively. Downregulation of NLK also inhibited tumorigenesis and regulated the expression of cell cycle protein expression levels. Therefore, it was hypothesized that NLK is necessary for cell survival and tumorigenesis in laryngeal cancer cells. Furthermore, the absence of NLK may lead to cancer cell death. Collectively, the results of the present study demonstrated that the lentivirus‑mediated targeted disruption of NLK may be a promising therapeutic method for the treatment of laryngeal cancer.

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