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Article

Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase

  • Authors:
    • Yao Yao
    • Juan Li
    • Yang Niu
    • Jian‑Qiang Yu
    • Ling Yan
    • Zhen‑Hua Miao
    • Xun‑Xia Zhao
    • Yuan‑Jie Li
    • Wan‑Xia Yao
    • Ping Zheng
    • Wei‑Qi Li
  • View Affiliations / Copyright

    Affiliations: Department of Medical Chemistry, School of Basic Medical Science, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Basic Traditional Chinese Medicine, School of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, National Resource Center of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, P.R. China
  • Pages: 6133-6139
    |
    Published online on: August 7, 2015
       https://doi.org/10.3892/mmr.2015.4199
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Abstract

Microglia‑mediated neuroinflammation is key in the pathogenesis of Alzheimer's disease (AD). Several studies have suggested that NADPH oxidase contributes to microglia‑mediated neuroinflammation. Resveratrol, which is a natural polyphenolic compound, exerts neuroprotective effects in AD due to its anti‑inflammatory properties. The present study aimed to investigate the effects of resveratrol on the activation of oligomeric amyloid β (oAβ)‑induced BV‑2 microglia, and to determine the role of NADPH oxidase in these effects. Microglial proliferation was measured by high‑content screening cell counting and using a bromodeoxyuridine incorporation assay. In addition, the levels of reactive oxygen species (ROS), nitric oxide (NO), tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β were assessed. The results of the present study demonstrated that resveratrol inhibited the proliferation of oAβ‑induced microglia and the production of pro‑inflammatory factors, including ROS, NO, TNF‑α and IL‑1β. Subsequent mechanistic investigations demonstrated that resveratrol inhibited the oAβ‑induced mRNA and protein expression levels of p47phox and gp91phox. These results suggested that NADPH oxidase may be a potential target for AD treatment, and resveratrol may be a valuable natural product possessing therapeutic potential against AD.
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1 

Glass CK, Saijo K, Winner B, Marchetto MC and Gage FH: Mechanisms underlying inflammation in neurodegeneration. Cell. 140:918–934. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Kawabori M and Yenari MA: The role of the microglia in acute CNS injury. Metab Brain Dis. 30:381–392. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Block ML, Zecca L and Hong JS: Microglia-mediated neurotoxicity: Uncovering the molecular mechanisms. Nat Rev Neurosci. 8:57–69. 2007. View Article : Google Scholar

4 

Markus MA and Morris BJ: Resveratrol in prevention and treatment of common clinical conditions of aging. Clin Interv Aging. 3:331–339. 2008.PubMed/NCBI

5 

Ponzo V, Soldati L and Bo S: Resveratrol: a supplementation for men or for mice? J Transl Med. 12:1582014. View Article : Google Scholar : PubMed/NCBI

6 

de la Lastra CA and Villegas I: Resveratrol as an anti-inflammatory and anti-aging agent: Mechanisms and clinical implications. Mol Nutr Food Res. 49:405–430. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Lorenz P, Roychowdhurys, Engelmann M, Wolf G and Horn TF: Oxyresveratrol and resveratrol are potent antioxidants and free radical scavengers: Effect on nitrosative and oxidative stress derived from microglial cells. Nitric Oxide. 9:64–76. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Bi XL, Yang JY, Dong YX, Wang JM, Cui YH, Ikeshima T, Zhao YQ and Wu CF: Resveratrol inhibits nitric oxide and TNF-alpha production by lipopolysaccharide-activated microglia. Int Immunopharmacol. 5:185–193. 2005. View Article : Google Scholar

9 

Candelario-Jalil E, de Oliveira AC, Gräfs, Bhatia HS, Hüll M, Muñoz E and Fiebich BL: Resveratrol potently reduces prostaglandin E2 production and free radical formation in lipopolysaccharide-activated primary rat microglia. J Neuroinfammation. 4:252007. View Article : Google Scholar

10 

Lu X, Ma L, Ruan L, Kong Y, Mou H, Zhang Z, Wang Z, Wang JM and Le Y: Resveratrol differentially modulates inflammatory responses of microglia and astrocytes. J Neuroinfammation. 7:462010. View Article : Google Scholar

11 

Zekry D, Epperson TK and Krause KH: A role for NOX NADPH oxidases in Alzheimer's disease and other types of dementia? IUBMB Life. 55:307–313. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Capiralla H, Vingtdeux V, Zhao H, Sankowski R, Al-Abed Y, Davies P and Marambaud P: Resveratrol mitigates lipopolysaccharide- and Aβ-mediated microglial infammation by inhibiting the TLR4/NF-κB/STAT signaling cascade. J Neurochem. 120:461–472. 2012. View Article : Google Scholar :

13 

Choi SH, Aid S, Kim HW, Jackson SH and Bosetti F: Inhibition of NADPH oxidase promotes alternative and anti-inflammatory microglial activation during neuroinflammation. J Neurochem. 120:292–301. 2012. View Article : Google Scholar :

14 

McLean CA, Cherny RA, Fraser FW, Fullers J, Smith MJ, Beyreuther K, Bush AI and Masters CL: Soluble pool of Abeta amyloid as a determinant of severity of neurodegeneration in Alzheimer's disease. Ann Neurol. 46:860–866. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Mc Donald JM, Savva GM, Brayne C, Welzel AT, Forster G, Shankar GM, Selkoe DJ, Ince PG and Walsh DM: Medical Research Council Cognitive Function and Ageing Study: The presence of sodium dodecyl sulphate-stable Abeta dimers is strongly associated with Alzheimer-type dementia. Brain. 133:1328–1341. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Li J, Yang JY, Yao XC, Xue X, Zhang QC, Wang XX, Ding LL and Wu CF: Oligomeric Aβ-induced microglial activation is possibly mediated by NADPH oxidase. Neurochem Res. 38:443–452. 2013. View Article : Google Scholar

17 

Bargers W and Harmon AD: Microglial activation by Alzheimer amyloid precursor protein and modulation by apolipoprotein E. Nature. 388:878–881. 1997. View Article : Google Scholar

18 

Groemping Y and Rittinger K: Activation and assembly of the NADPH oxidase: A structural perspective. Biochem J. 386:401–416. 2005. View Article : Google Scholar :

19 

Giuliani F, Hader W and Yong VW: Minocycline attenuates T cell and microglia activity to impair cytokine production in T cell-microglia interaction. J Leukoc Biol. 78:135–143. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Maezawa I, Zimin PI, Wulff H and Jin LW: Amyloid-beta protein oligomer at low nanomolar concentrations activates microglia and induces microglial neurotoxicity. J Biol Chem. 286:3693–3706. 2011. View Article : Google Scholar :

21 

Jekabsone A, Mander PK, Tickler A, Sharpe M and Brown GC: Fibrillar beta-amyloid peptide Abeta1–40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: A cell culture study. J Neuroinflamm. 3:242006. View Article : Google Scholar

22 

Green SP, Cairns B, Rae J, Errett-Baroncini C, Hongo JA, Erickson RW and Curnutte JT: Induction of gp91-phox, a component of the phagocyte NADPH oxidase, in microglial cells during central nervous system inflammation. J Cereb Blood Flow Metab. 21:374–384. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Klegeris A and McGeer PL: Rat brain microglia and peritoneal macrophages show similar responses to respiratory burst stimulants. J Neuroimmunol. 53:83–90. 1994. View Article : Google Scholar : PubMed/NCBI

24 

Babior BM: Oxidants from phagocytes: Agents of defense and destruction. Blood. 64:959–966. 1984.PubMed/NCBI

25 

Qin L, Liu Y, Wang T, Wei SJ, Block ML, Wilson B, Liu B and Hong JS: NADPH oxidase mediates lipopolysaccharide-induced neurotoxicity and proinflammatory gene expression in activated microglia. J Biol Chem. 279:1415–1421. 2004. View Article : Google Scholar

26 

Leonard SS, Xia C, Jiang BH, Stinefelt B, Klandorf H, Harris GK and Shi X: Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochem Biophys Res Commun. 309:1017–1026. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Spanier G, Xu H, Xia N, Tobias S, Deng S, Wojnowski L, Forstermann U and Li H: Resveratrol reduces endothelial oxidative stress by modulating the gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4). J Physiol Pharmacol. 60(Suppl 4): 111–116. 2009.

28 

Banati RB, Gehrmann J, Schubert P and Kreutzberg GW: Cytotoxicity of microglia. Glia. 7:111–118. 1993. View Article : Google Scholar : PubMed/NCBI

29 

Hur J, Lee P, Kim MJ, Kim Y and Cho YW: Ischemia-activated microglia induces neuronal injury via activation of gp91phox NADPH oxidase. Biochem Biophys Res Commun. 391:1526–1530. 2010. View Article : Google Scholar

30 

Wilms H, Sievers J, Rickert U, Rostami-Yazdi M, Mrowietz U and Lucius R: Dimethylflumarate inhibits microglial and astrocytic inflammation by suppressing the synthesis of nitric oxide, IL-1beta, TNF-alpha and IL-6 in an in-vitro model of brain inflammation. J Neuroinfammation. 7:302010. View Article : Google Scholar

31 

Eikelenboom P, Veerhuis R, Scheper W, Rozemuller AJ, van Gool WA and Hoozemans JJ: The significance of neuroinflammation in understanding Alzheimer's disease. J Neural Transm. 113:1685–1695. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao Y, Li J, Niu Y, Yu JQ, Yan L, Miao ZH, Zhao XX, Li YJ, Yao WX, Zheng P, Zheng P, et al: Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase. Mol Med Rep 12: 6133-6139, 2015.
APA
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z. ... Li, W. (2015). Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase. Molecular Medicine Reports, 12, 6133-6139. https://doi.org/10.3892/mmr.2015.4199
MLA
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z., Zhao, X., Li, Y., Yao, W., Zheng, P., Li, W."Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase". Molecular Medicine Reports 12.4 (2015): 6133-6139.
Chicago
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z., Zhao, X., Li, Y., Yao, W., Zheng, P., Li, W."Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase". Molecular Medicine Reports 12, no. 4 (2015): 6133-6139. https://doi.org/10.3892/mmr.2015.4199
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Y, Li J, Niu Y, Yu JQ, Yan L, Miao ZH, Zhao XX, Li YJ, Yao WX, Zheng P, Zheng P, et al: Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase. Mol Med Rep 12: 6133-6139, 2015.
APA
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z. ... Li, W. (2015). Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase. Molecular Medicine Reports, 12, 6133-6139. https://doi.org/10.3892/mmr.2015.4199
MLA
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z., Zhao, X., Li, Y., Yao, W., Zheng, P., Li, W."Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase". Molecular Medicine Reports 12.4 (2015): 6133-6139.
Chicago
Yao, Y., Li, J., Niu, Y., Yu, J., Yan, L., Miao, Z., Zhao, X., Li, Y., Yao, W., Zheng, P., Li, W."Resveratrol inhibits oligomeric Aβ‑induced microglial activation via NADPH oxidase". Molecular Medicine Reports 12, no. 4 (2015): 6133-6139. https://doi.org/10.3892/mmr.2015.4199
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