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Article

Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells

  • Authors:
    • Ping Ma
    • Qianying Gao
    • Zhichong Wang
    • Keming Yu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China
  • Pages: 6025-6030
    |
    Published online on: August 10, 2015
       https://doi.org/10.3892/mmr.2015.4205
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Abstract

Members of the protein kinase C (PKC) family are involved in physiological and pathophysiological processes, and exert an important role in signal transduction. The aim of the present study was to determine which of the 12 protein kinase C (PKC) isoforms (PKCα, PKCβⅠ, PKCβⅡ, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCµ, PKCζ, PKCλ and PKCι) were expressed in vitro in cultured human Tenon's capsule fibroblasts (HTFs). HTFs from cell passages three to five were investigated for the presence of the 12 PKC isoforms at the cellular, mRNA and protein levels using laser scanning confocal microscopy (LSCM), reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis, respectively. These analyses yielded similar results for several of the PKC isoforms (PKCα, PKCδ, PKCε, PKCη, PKCµ, PKCζ, PKCλ and PKCι); however, PKCβⅠ, PKCβⅡ, PKCγ and PKCθ were not expressed in the cultured HTFs. LSCM revealed that eight PKC isoforms, PKCα, PKCδ, PKCε, PKCη, PKCµ, PKCζ, PKCλ and PKCι, appeared almost exclusively in the cytoplasm of the cells. Notably, PKCδ was expressed particularly well in the cytoskeleton. The present study revealed that all 12 PKC isoforms were expressed and that eight of the isoforms were present in cultured HTFs. These results will be of value when determining specific roles for the PKC isoforms in HTF proliferation, which may provide a novel therapeutic target for bleb scarring in glaucoma filtering surgery.
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Copy and paste a formatted citation
Spandidos Publications style
Ma P, Gao Q, Wang Z and Yu K: Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells. Mol Med Rep 12: 6025-6030, 2015.
APA
Ma, P., Gao, Q., Wang, Z., & Yu, K. (2015). Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells. Molecular Medicine Reports, 12, 6025-6030. https://doi.org/10.3892/mmr.2015.4205
MLA
Ma, P., Gao, Q., Wang, Z., Yu, K."Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells". Molecular Medicine Reports 12.4 (2015): 6025-6030.
Chicago
Ma, P., Gao, Q., Wang, Z., Yu, K."Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells". Molecular Medicine Reports 12, no. 4 (2015): 6025-6030. https://doi.org/10.3892/mmr.2015.4205
Copy and paste a formatted citation
x
Spandidos Publications style
Ma P, Gao Q, Wang Z and Yu K: Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells. Mol Med Rep 12: 6025-6030, 2015.
APA
Ma, P., Gao, Q., Wang, Z., & Yu, K. (2015). Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells. Molecular Medicine Reports, 12, 6025-6030. https://doi.org/10.3892/mmr.2015.4205
MLA
Ma, P., Gao, Q., Wang, Z., Yu, K."Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells". Molecular Medicine Reports 12.4 (2015): 6025-6030.
Chicago
Ma, P., Gao, Q., Wang, Z., Yu, K."Expression of protein kinase C isoforms in cultured human tenon's capsule fibroblast cells". Molecular Medicine Reports 12, no. 4 (2015): 6025-6030. https://doi.org/10.3892/mmr.2015.4205
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