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Article

Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells

  • Authors:
    • Yunpeng Cui
    • Feng Yang
    • Lu He
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China, Department of Neurosurgery, The Sixth People's Hospital of Chongqing City, Chongqing 400060, P.R. China, Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, P.R. China
  • Pages: 7027-7032
    |
    Published online on: August 21, 2015
       https://doi.org/10.3892/mmr.2015.4236
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Abstract

Despite advances in the development of treatment methods, glioma remains among the cancer types with a high rate of mortality. Therefore, significant efforts are made to develop novel strategies for the treatment of glioma. Ineffective, long-term cancer chemotherapy can lead to multidrug resistance (MDR), which is one of the most common reasons for the failure of chemotherapy. The present study investigated the effects of cytokine‑induced killer cells (CIK) on reversing MDR in cisplatin-resistant U87MG cells (U87MG/DDP). Mononuclear cells were isolated from the peripheral blood of healthy individuals and cultured in vitro in the presence of a combination of cytokines to generate CIK for the treatment of U87MG/DDP. An MTS assay, flow cytometric analysis of apoptosis, ELISA, western blotting and reverse transcription quantitative polymerase chain reaction were used to investigate the MDR-reversing effects of CIK as well as the underlying mechanisms. The results showed that cisplatin sensitivity and the apoptotic rate following cisplatin treatment were increased, P‑glycoprotein expression was decreased and the intracellular rhodamine‑123 content was increased in U87MG/DDP co‑cultured with CIK. In addition, the present study also identified increased mRNA and protein expression levels of MDR gene 1 (MDR1), MDR‑associated protein 1 (MRP1), B-cell lymphoma 2, Survivin and glutathione S-transferase‑π, while the phosphorylation of AKT and the transcriptional activity of nuclear factor‑κB in CIK co‑cultured U87MG/DDP was decreased. These results indicated that pre‑treatment with CIK reversed the MDR of U87MG/DDP, and that CIK‑induced apoptosis of U87MG/DDP was associated with the inhibition of Akt/NF‑κB. These findings suggested that treatment with CIK may be an effective method to enhance the sensitivity of patients with glioma to chemotherapy.
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1 

Fan QW and Weiss WA: Targeting the RTK-PI3K-mTOR axis in malignant glioma: Overcoming resistance. Curr Top Microbiol Immunol. 347:279–296. 2010.PubMed/NCBI

2 

Desjardins A, Rich JN, Quinn JA, Vredenburgh J, Gururangan S, Sathornsumetee S, Reardon DA, Friedman AH, Bigner DD and Friedman HS: Chemotherapy and novel therapeutic approaches in malignant glioma. Front Biosci. 10:2645–2668. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Curiel TJ: Immunotherapy: A useful strategy to help combat multidrug resistance. Drug Resist Updat. 15:106–113. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Ji J, Black KL and Yu JS: Glioma stem cell research for the development of immunotherapy. Neurosurg Clin N Am. 21:159–166. 2010. View Article : Google Scholar

5 

Tu W, Cheung PT and Lau YL: Insulin-like growth factor 1 promotes cord blood T cell maturation and inhibits its spontaneous and phytohemagglutinin-induced apoptosis through different mechanisms. J Immunol. 165:1331–1336. 2000. View Article : Google Scholar : PubMed/NCBI

6 

Kim JS, Chung IS, Lim SH, Park Y, Park MJ, Kim JY, Kim YG, Hong JT, Kim Y and Han SB: Preclinical and clinical studies on cytokine-induced killer cells for the treatment of renal cell carcinoma. Arch Pharm Res. 37:559–566. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Rettinger E, Kreyenberg H, Merker M, Kuci S, Willasch A, Bug G, Ullrich E, Wels WS, Bonig H, Klingebiel T and Bader P: Immunomagnetic selection or irradiation eliminates alloreactive cells but also reduces anti-tumor potential of cytokine-induced killer cells: Implications for unmanipulated cytokine-induced killer cell infusion. Cytotherapy. 16:835–844. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Zhou W, Fu XQ, Zhang LL, Zhang J, Huang X, Lu XH, Shen L, Liu BN, Liu J, Luo HS, et al: The AKT1/NF-kappaB/Notch1/PTEN axis has an important role in chemoresistance of gastric cancer cells. Cell Death Dis. 4:e8472013. View Article : Google Scholar : PubMed/NCBI

9 

Wang Y, Li CF, Pan LM and Gao ZL: 7,8-Dihydroxycoumarin inhibits A549 human lung adenocarcinoma cell proliferation by inducing apoptosis via suppression of Akt/NF-κB signaling. Exp Ther Med. 5:1770–1774. 2013.PubMed/NCBI

10 

Piil K, Juhler M, Jakobsen J and Jarden M: Controlled rehabilitative and supportive care intervention trials in patients with high-grade gliomas and their caregivers: A systematic review. BMJ Support Palliat Care. pii: bmjspcare-2013-000593. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Dirven L, Aaronson NK, Heimans JJ and Taphoorn MJ: Health-related quality of life in high-grade glioma patients. Chin J Cancer. 33:40–45. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Melin B and Jenkins R: Genetics in glioma: Lessons learned from genome-wide association studies. Curr Opin Neurol. 26:688–692. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Li W, Holsinger RM, Kruse CA, Flügel A and Graeber MB: The potential for genetically altered microglia to influence glioma treatment. CNS Neurol Disord Drug Targets. 12:750–762. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Zhang J, Zhu L, Zhang Q, He X, Yin Y, Gu Y, Guo R, Lu K, Liu L, Liu P and Shu Y: Effects of cytokine-induced killer cell treatment in colorectal cancer patients: A retrospective study. Biomed Pharmacother. 68:715–720. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Queiroz RM, Takiya CM, Guimarães LP, Rocha Gda G, Alviano DS, Blank AF, Alviano CS and Gattass CR: Apoptosis-inducing effects of melissa officinalis L. essential oil in glioblastoma multiforme cells. Cancer Invest. 32:226–235. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Zhu Y, Liu XJ, Yang P, Zhao M, Lv LX, Zhang GD, Wang Q and Zhang L: Alkylglyceronephosphate synthase (AGPS) alters lipid signaling pathways and supports chemotherapy resistance of glioma and hepatic carcinoma cell lines. Asian Pac J Cancer Prev. 15:3219–3226. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Nakai E, Park K, Yawata T, Chihara T, Kumazawa A, Nakabayashi H and Shimizu K: Enhanced MDR1 expression and chemoresistance of cancer stem cells derived from glioblastoma. Cancer Invest. 27:901–908. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Goda K, Bacsó Z and Szabó G: Multidrug resistance through the spectacle of P-glycoprotein. Curr Cancer Drug Targets. 9:281–297. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Lage H: MDR1/P-glycoprotein (ABCB1) as target for RNA interference-mediated reversal of multidrug resistance. Curr Drug Targets. 7:813–821. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Xu D, Tian W and Shen H: Curcumin prevents induced drug resistance: A novel function? Chin J Cancer Res. 23:218–223. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Wang L, Deng Q, Wang J, Bai X, Xiao X, Lv HR, Zhao MF and Liu PJ: Effect of CIK on multidrug-resistance reversal and increasing the sensitivity of ADR in K562/ADR cells. Oncol Lett. 8:1778–1782. 2014.PubMed/NCBI

22 

Peklak-Scott C, Smitherman PK, Townsend AJ and Morrow CS: Role of glutathione S-transferase P1-1 in the cellular detoxification of cisplatin. Mol Cancer Ther. 7:3247–3255. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Calatozzolo C, Gelati M, Ciusani E, Sciacca FL, Pollo B, Cajola L, Marras C, Silvani A, Vitellaro-Zuccarello L, Croci D, et al: Expression of drug resistance proteins Pgp, MRP1, MRP3, MRP5 and GST-pi in human glioma. J Neurooncol. 74:113–121. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cui Y, Yang F and He L: Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells. Mol Med Rep 12: 7027-7032, 2015.
APA
Cui, Y., Yang, F., & He, L. (2015). Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells. Molecular Medicine Reports, 12, 7027-7032. https://doi.org/10.3892/mmr.2015.4236
MLA
Cui, Y., Yang, F., He, L."Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells". Molecular Medicine Reports 12.5 (2015): 7027-7032.
Chicago
Cui, Y., Yang, F., He, L."Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells". Molecular Medicine Reports 12, no. 5 (2015): 7027-7032. https://doi.org/10.3892/mmr.2015.4236
Copy and paste a formatted citation
x
Spandidos Publications style
Cui Y, Yang F and He L: Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells. Mol Med Rep 12: 7027-7032, 2015.
APA
Cui, Y., Yang, F., & He, L. (2015). Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells. Molecular Medicine Reports, 12, 7027-7032. https://doi.org/10.3892/mmr.2015.4236
MLA
Cui, Y., Yang, F., He, L."Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells". Molecular Medicine Reports 12.5 (2015): 7027-7032.
Chicago
Cui, Y., Yang, F., He, L."Cytokine-induced killer cells induce apoptosis and inhibit the Akt/nuclear factor-κB signaling pathway in cisplatin-resistant human glioma U87MG cells". Molecular Medicine Reports 12, no. 5 (2015): 7027-7032. https://doi.org/10.3892/mmr.2015.4236
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