Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library

Wang,Wei; Chen,Tao; Li,Haicheng; Chen,Yuhui; Wu,Zhilong; Feng,Tongming; Zhang,Xilin; Zhong,Qiu; Zhong,Qianhong; Li,Guozhou; Guo,Lina; Zhou,Lin; Zhou,Jie

doi:10.3892/mmr.2015.4248

November-2015 Volume 12 Issue 5

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November-2015 Volume 12 Issue 5

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Article

Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library

Authors:
- Wei Wang
- Tao Chen
- Haicheng Li
- Yuhui Chen
- Zhilong Wu
- Tongming Feng
- Xilin Zhang
- Qiu Zhong
- Qianhong Zhong
- Guozhou Li
- Lina Guo
- Lin Zhou
- Jie Zhou
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory and Disease Control, Foshan Fourth People's Hospital, Foshan, Guangdong 528000, P.R. China, Department of Provincial Reference Laboratory and Disease Control, Center for Tuberculosis Control of Guangdong, Guangzhou, Guangdong 510630, P.R. China, Department of Clinical Laboratory, Chronic Disease Control and Prevention Station of Dongguan, Dongguan, Guangdong 523008, P.R. China, Department of Nutrition, Guangdong Provincial Hospital of Chinese Traditional Medicine, Guangzhou, Guangdong 510120, P.R. China
Pages: 7051-7058
|
Published online on: August 25, 2015

https://doi.org/10.3892/mmr.2015.4248
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Abstract

Accumulating evidence has suggested that fibroblast growth factor 3 (FGF3) is expressed in breast cancer and correlates with the stage and grade of the disease. In the present study, a specific FGF3‑binding peptide (VLWLKNR, termed FP16) was isolated from a phage display heptapeptide library with FGF3. The peptide FP16 contained four identical (WLKN) amino acids and demonstrated high homology to the peptides of the 188‑194 (TMRWLKN) site of the high‑affinity FGF3 receptor fibroblast growth factor receptor 2. Functional analyses indicated that FP16 mediated significant inhibition of FGF3‑induced cell proliferation, arrested the cell cycle at the G0/G1 phase by increasing proliferation‑associated protein 2G4, suppressing cyclin D1 and proliferating cell nuclear antigen, and inhibited the FGF3‑induced activation of extracellular signal‑regulated kinase 1/2 and Akt kinase. Taken together, these results demonstrated that the peptide FP16, acting as an FGF3 antagonist, is a promising therapeutic agent for the treatment of breast cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library

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