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DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia

  • Authors:
    • Qi Liao
    • Yunliang Wang
    • Jia Cheng
    • Dongjun Dai
    • Xingyu Zhou
    • Yuzheng Zhang
    • Jinfeng Li
    • Honglei Yin
    • Shugui Gao
    • Shiwei Duan
  • View Affiliations / Copyright

    Affiliations: Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Neurology, The 148th Central Hospital of the People's Liberation Army, Zibo, Shandong 255300, P.R. China, Department of Psychiatry, Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6568-6576
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    Published online on: August 25, 2015
       https://doi.org/10.3892/mmr.2015.4249
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Abstract

Schizophrenia (SCZ) is one of the most complex mental illnesses affecting ~1% of the population worldwide. SCZ pathogenesis is considered to be a result of genetic as well as epigenetic alterations. Previous studies have aimed to identify the causative genes of SCZ. However, DNA methylation of long non‑coding RNAs (lncRNAs) involved in SCZ has not been fully elucidated. In the present study, a comprehensive genome‑wide analysis of DNA methylation was conducted using samples from two male patients with paranoid and undifferentiated SCZ, respectively. Methyl‑CpG binding domain protein‑enriched genome sequencing was used. In the two patients with paranoid and undifferentiated SCZ, 1,397 and 1,437 peaks were identified, respectively. Bioinformatic analysis demonstrated that peaks were enriched in protein‑coding genes, which exhibited nervous system and brain functions. A number of these peaks in gene promoter regions may affect gene expression and, therefore, influence SCZ‑associated pathways. Furthermore, 7 and 20 lncRNAs, respectively, in the Refseq database were hypermethylated. According to the lncRNA dataset in the NONCODE database, ~30% of intergenic peaks overlapped with novel lncRNA loci. The results of the present study demonstrated that aberrant hypermethylation of lncRNA genes may be an important epigenetic factor associated with SCZ. However, further studies using larger sample sizes are required.
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Spandidos Publications style
Liao Q, Wang Y, Cheng J, Dai D, Zhou X, Zhang Y, Li J, Yin H, Gao S, Duan S, Duan S, et al: DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia. Mol Med Rep 12: 6568-6576, 2015.
APA
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y. ... Duan, S. (2015). DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia. Molecular Medicine Reports, 12, 6568-6576. https://doi.org/10.3892/mmr.2015.4249
MLA
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y., Li, J., Yin, H., Gao, S., Duan, S."DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia". Molecular Medicine Reports 12.5 (2015): 6568-6576.
Chicago
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y., Li, J., Yin, H., Gao, S., Duan, S."DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia". Molecular Medicine Reports 12, no. 5 (2015): 6568-6576. https://doi.org/10.3892/mmr.2015.4249
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Spandidos Publications style
Liao Q, Wang Y, Cheng J, Dai D, Zhou X, Zhang Y, Li J, Yin H, Gao S, Duan S, Duan S, et al: DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia. Mol Med Rep 12: 6568-6576, 2015.
APA
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y. ... Duan, S. (2015). DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia. Molecular Medicine Reports, 12, 6568-6576. https://doi.org/10.3892/mmr.2015.4249
MLA
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y., Li, J., Yin, H., Gao, S., Duan, S."DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia". Molecular Medicine Reports 12.5 (2015): 6568-6576.
Chicago
Liao, Q., Wang, Y., Cheng, J., Dai, D., Zhou, X., Zhang, Y., Li, J., Yin, H., Gao, S., Duan, S."DNA methylation patterns of protein-coding genes and long non-coding RNAs in males with schizophrenia". Molecular Medicine Reports 12, no. 5 (2015): 6568-6576. https://doi.org/10.3892/mmr.2015.4249
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