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November-2015 Volume 12 Issue 5

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Article

De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer

  • Authors:
    • Jinhang Gao
    • Shilei Wen
    • Hongying Zhou
    • Shi Feng
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Academy of Preclinical and Forensic Medicine, West China Medicine College, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 7033-7038
    |
    Published online on: August 26, 2015
       https://doi.org/10.3892/mmr.2015.4256
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Abstract

DNA methylation occurs in the displacement loop (D-loop) region of mammals; however, D-loop regions of certain tumor tissue types were found to be de‑methylated. Whether hypomethylation of the D‑loop region is involved in the regulation of the mitochondrial DNA (mtDNA) copy number and nicotinamide adenine dinucleotide subunit 2 (ND‑2) expressions in colorectal cancer has remained elusive. In the present study, the association between methylation status of the D‑loop region, mtDNA copy number and ND‑2 expression was investigated in 65 colorectal cancer specimens and their corresponding non‑cancerous tissues. In addition, a de‑methylation experiment was performed on the Caco‑2 colorectal cancer cell line by using 5‑aza-2'-deoxycytidine (5‑Aza). The methylation rate of the D‑loop region in all 65 colorectal cancer tissues was markedly reduced when compared with that of their corresponding non‑cancerous tissues (13.8 vs. 81.5%; P<0.05). Furthermore, the methylation rate of the D‑loop region in colorectal cancer tissues was markedly decreased in clinicopathological stages III and IV compared with that in clinicopathological stages I and II (7.1 and 0% vs. 25 and 16%; P<0.05). In addition, the mean relative mtDNA copy number and ND‑2 expression in colorectal cancer tissues were increased compared with those in the corresponding non‑cancerous tissues. De‑methylation of the D‑loop region was associated with an elevated mtDNA copy number and an increased ND‑2 expression. Furthermore, the mtDNA copy number and ND‑2 expression in Caco‑2 cells were significantly increased after 5‑Aza treatment. In conclusion, de‑methylation of the D‑loop region is likely to be involved in the regulation of the mtDNA copy number and ND-2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Gao J, Wen S, Zhou H and Feng S: De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer. Mol Med Rep 12: 7033-7038, 2015.
APA
Gao, J., Wen, S., Zhou, H., & Feng, S. (2015). De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer. Molecular Medicine Reports, 12, 7033-7038. https://doi.org/10.3892/mmr.2015.4256
MLA
Gao, J., Wen, S., Zhou, H., Feng, S."De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer". Molecular Medicine Reports 12.5 (2015): 7033-7038.
Chicago
Gao, J., Wen, S., Zhou, H., Feng, S."De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer". Molecular Medicine Reports 12, no. 5 (2015): 7033-7038. https://doi.org/10.3892/mmr.2015.4256
Copy and paste a formatted citation
x
Spandidos Publications style
Gao J, Wen S, Zhou H and Feng S: De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer. Mol Med Rep 12: 7033-7038, 2015.
APA
Gao, J., Wen, S., Zhou, H., & Feng, S. (2015). De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer. Molecular Medicine Reports, 12, 7033-7038. https://doi.org/10.3892/mmr.2015.4256
MLA
Gao, J., Wen, S., Zhou, H., Feng, S."De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer". Molecular Medicine Reports 12.5 (2015): 7033-7038.
Chicago
Gao, J., Wen, S., Zhou, H., Feng, S."De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer". Molecular Medicine Reports 12, no. 5 (2015): 7033-7038. https://doi.org/10.3892/mmr.2015.4256
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