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Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease

  • Authors:
    • Wanhua Hu
    • Xiaodong Lin
    • Kelong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Wenzhou Hospital of Traditional Chinese Medicine, Wenzhou, Zhejiang 325000, P.R. China, Department of Traditional Chinese Internal Medicine, Wenzhou Seventh People's Hospital, Wenzhou, Zhejiang 325005, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6679-6687
    |
    Published online on: August 28, 2015
       https://doi.org/10.3892/mmr.2015.4271
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Abstract

Alzheimer's disease (AD) is a complex neurodegenerative disorder with largely unknown genetic mechanisms. Identifying altered neuronal gene expression in AD may provide diagnostic or therapeutic targets for AD. The present study aimed to identify differentially expressed genes (DEGs) and their further association with other biological processes that regulate causative factors for AD. The present study performed an integrated analysis of publicly available gene expression omnibus datasets of AD hippocampi. Gene ontology (GO) enrichment analyses, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Protein‑Protein interaction (PPI) network analysis were performed. The present study detected 295 DEGs (109 upregulated and 186 downregulated genes) in hippocampi between AD and control samples by integrating four datasets of gene expression profiles of hippocampi of patients with AD. Respiratory electron transport chain (GO: 0022904; P=1.64x10‑11) was the most significantly enriched GO term among biological processes, while for molecular functions, the most significantly enriched GO term was that of protein binding (GO: 0005515; P=3.03x10‑29), and for cellular components, the most significantly enriched GO term was that of the cytoplasm (GO: 0005737; P=8.67x10‑33). The most significant pathway in the KEGG analysis was oxidative phosphorylation (P=1.61x10‑13). PPI network analysis showed that the significant hub proteins contained β-actin (degree, 268), hepatoma-derived growth factor (degree, 218) and WD repeat‑containing protein 82 (degree, 87). The integrated analysis performed in the present study serves as a basis for identifying novel drug targets to develop improved therapies and interventions for common and devastating neurological diseases such as AD.
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Copy and paste a formatted citation
Spandidos Publications style
Hu W, Lin X and Chen K: Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease. Mol Med Rep 12: 6679-6687, 2015.
APA
Hu, W., Lin, X., & Chen, K. (2015). Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease. Molecular Medicine Reports, 12, 6679-6687. https://doi.org/10.3892/mmr.2015.4271
MLA
Hu, W., Lin, X., Chen, K."Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease". Molecular Medicine Reports 12.5 (2015): 6679-6687.
Chicago
Hu, W., Lin, X., Chen, K."Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease". Molecular Medicine Reports 12, no. 5 (2015): 6679-6687. https://doi.org/10.3892/mmr.2015.4271
Copy and paste a formatted citation
x
Spandidos Publications style
Hu W, Lin X and Chen K: Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease. Mol Med Rep 12: 6679-6687, 2015.
APA
Hu, W., Lin, X., & Chen, K. (2015). Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease. Molecular Medicine Reports, 12, 6679-6687. https://doi.org/10.3892/mmr.2015.4271
MLA
Hu, W., Lin, X., Chen, K."Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease". Molecular Medicine Reports 12.5 (2015): 6679-6687.
Chicago
Hu, W., Lin, X., Chen, K."Integrated analysis of differential gene expression profiles in hippocampi to identify candidate genes involved in Alzheimer's disease". Molecular Medicine Reports 12, no. 5 (2015): 6679-6687. https://doi.org/10.3892/mmr.2015.4271
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