Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice

Jiang,Yanhua; Fu,Rongrong; Zhao,Jiangyue; Wu,Di; Qiao,Guang; Li,Ruoxi; Zhang,Jinsong

doi:10.3892/mmr.2015.4281

Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice

Authors:
- Yanhua Jiang
- Rongrong Fu
- Jiangyue Zhao
- Di Wu
- Guang Qiao
- Ruoxi Li
- Jinsong Zhang
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Affiliations: Department of Ophthalmology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110005, P.R. China, Department of Ophthalmology, The Fourth People's Hospital of Shenyang, Shenyang, Liaoning 110031, P.R. China
Published online on: September 2, 2015 https://doi.org/10.3892/mmr.2015.4281
Pages: 6605-6611
Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

ELL-associated factor 2 (Eaf2) has an important role in crystalline lens development and maturation; however, its role in ultraviolet radiation (UV)-induced cataract formation has remained elusive. The present study compared UV-induced cell apoptosis, activation of caspase-3 and caspase-9 and changes in protein expression levels of B-cell lymphoma 2 (bcl-2), bcl-2-associated X protein (bax) and phosphorylated extracellular signal-regulated kinase in wild-type and Eaf2-knockout mice. The results showed that Eaf2 knockout can reduce UV-induced apoptosis in crystalline lenses and mitigate the formation of cataracts. Further functional studies indicated that Eaf2 can induce the activation of caspase-3 and caspase-9, increase the protein expression of the pro-apoptotic protein bax and inhibit the expression of the anti-apoptotic protein bcl-2; thereby, Eaf2 promotes cell apoptosis and is implicated in the formation and development of cataracts. The present study laid a theoretical foundation for the development of drugs for cataract treatment.

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