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Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy

  • Authors:
    • Jun Huang
    • Yujiao He
    • Mingna Chen
    • Juan Du
    • Guoliang Li
    • Shuyu Li
    • Weiping Liu
    • Xiaoyan Long
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Neurology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6509-6516
    |
    Published online on: September 2, 2015
       https://doi.org/10.3892/mmr.2015.4285
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Abstract

The aim of the present study was to investigate adenosine deaminase (ADA) and adenosine kinase (ADK) expression in human glioma and to explore its correlation with glioma‑associated epilepsy. Tumor tissues (n=45) and peritumoral tissues (n=14) were obtained from glioma patients undergoing surgery. Normal control tissues (n=8) were obtained from brain trauma patients. The disease grade was determined by histological evaluation and the degree of tumor invasion was evaluated using immunofluorescence analyses. mRNA and protein expression of ADA and ADK were evaluated using reverse transcription quantitative polymerase chain reaction or western blot analysis, respectively. Based on histological evaluations, four cases were classified as Grade I gliomas, 18 cases as Grade II, 17 cases as Grade III and six cases were considered Grade IV. Increased ADA and ADK expression was observed in tumor tissues. ADA was predominantly distributed in the cytoplasm of tumor cells, whereas ADK was detected in the cytoplasm as well as in the nuclei. ADA and ADK levels were upregulated in patients with Grade II and Grade III gliomas compared to those in control subjects (p<0.05). In addition, tumor invasion was detected in peritumoral tissues. The number of ADA‑positive or ADK‑positive cells in tumor tissues was similar between glioma patients with and without epilepsy (p>0.05). However, ADA and ADK expression was upregulated in peritumoral tissues derived from patients with epilepsy compared to that in glioma patients without epilepsy. The results of the present study suggested that ADA and ADK are involved in glioma progression, and that increased ADA and ADK levels in peritumoral tissues may be associated with epilepsy in glioma patients.
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1 

Pace A, Bove L, Innocenti P, Pietrangeli A, Carapella CM, Oppido P, Raus L, Occhipinti E and Jandolo B: Epilepsy and gliomas: Incidence and treatment in 119 patients. J Exp Clin Cancer Res. 17:479–482. 1998.

2 

Behin A, Hoang-Xuan K, Carpentier AF and Delattre JY: Primary brain tumours in adults. Lancet. 361:323–331. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Pallud J, Audureau E, Blonski M, Sanai N, Bauchet L, Fontaine D, Mandonnet E, Dezamis E, Psimaras D, Guyotat J, et al: Epileptic seizures in diffuse low-grade gliomas in adults. Brain. 137:449–462. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Berntsson SG, Malmer B, Bondy ML, Qu M and Smits A: Tumor-associated epilepsy and glioma: Are there common genetic pathways? Acta Oncol. 48:955–963. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Lynam LM, Lyons MK, Drazkowski JF, Sirven JI, Noe KH, Zimmerman RS and Wilkens JA: Frequency of seizures in patients with newly diagnosed brain tumors: A retrospective review. Clin Neurol Neurosurg. 109:634–638. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Stieber VW: Low-grade gliomas. Curr Treat Options Oncol. 2:495–506. 2001. View Article : Google Scholar

7 

Lee MC, Kim GM, Woo YJ, Kim MK, Kim JH, Nam SC, Suh JJ, Chung WK, Lee JS, Kim HI, et al: Pathogenic significance of neuronal migration disorders in temporal lobe epilepsy. Hum Pathol. 32:643–648. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B and Troost D: Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol. 101:449–459. 2001.PubMed/NCBI

9 

Yuen TI, Morokoff AP, Bjorksten A, D'Abaco G, Paradiso L, Finch S, Wong D, Reid CA, Powell KL, Drummond KJ, et al: Glutamate is associated with a higher risk of seizures in patients with gliomas. Neurology. 79:883–889. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Boison D: Role of adenosine in status epilepticus: A potential new target? Epilepsia. 54(Suppl 6): 20–22. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Boison D: Adenosine and seizure termination: Endogenous mechanisms. Epilepsy Curr. 13:35–37. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Hargus NJ, Jennings C, Perez-Reyes E, Bertram EH and Patel MK: Enhanced actions of adenosine in medial entorhinal cortex layer II stellate neurons in temporal lobe epilepsy are mediated via A(1)-receptor activation. Epilepsia. 53:168–176. 2012. View Article : Google Scholar :

13 

Boison D: Adenosine kinase: Exploitation for therapeutic gain. Pharmacol Rev. 65:906–943. 2013. View Article : Google Scholar : PubMed/NCBI

14 

de Groot M, Iyer A, Zurolo E, Anink J, Heimans JJ, Boison D, Reijneveld JC and Aronica E: Overexpression of ADK in human astrocytic tumors and peritumoral tissue is related to tumor-associated epilepsy. Epilepsia. 53:58–66. 2012. View Article : Google Scholar :

15 

Siebel AM, Piato AL, Schaefer IC, Nery LR, Bogo MR and Bonan CD: Antiepileptic drugs prevent changes in adenosine deamination during acute seizure episodes in adult zebrafish. Pharmacol Biochem Behav. 104:20–26. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW and Kleihues P: The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 114:97–109. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

18 

Jinnah HA, Sabina RL and Van Den Berghe G: Metabolic disorders of purine metabolism affecting the nervous system. Handb Clin Neurol. 113:1827–1836. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Bardot V, Dutrillaux AM, Delattre JY, Vega F, Poisson M, Dutrillaux B and Luccioni C: Purine and pyrimidine metabolism in human gliomas: Relation to chromosomal aberrations. Br J Cancer. 70:212–218. 1994. View Article : Google Scholar : PubMed/NCBI

20 

Castillo CA, León D, Ruiz MA, Albasanz JL and Martín M: Modulation of adenosine A1 and A2A receptors in C6 glioma cells during hypoxia: Involvement of endogenous adenosine. J Neurochem. 105:2315–2329. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Ohkubo S, Nagata K and Nakahata N: Adenosine uptake-dependent C6 cell growth inhibition. Eur J Pharmacol. 577:35–43. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Swiader MJ, Kotowski J and Łuszczki JJ: Modulation of adenosinergic system and its application for the treatment of epilepsy. Pharmacol Rep. 66:335–342. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Pence S, Erkutlu I, Kurtul N, Bosnak M, Alptekin M and Tan U: Antiepileptogenic effects of glutathione against increased brain ADA in PTZ-induced epilepsy. Int J Neurosci. 119:616–629. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Shen HY, Sun H, Hanthorn MM, Zhi Z, Lan JQ, Poulsen DJ, Wang RK and Boison D: Overexpression of adenosine kinase in cortical astrocytes and focal neocortical epilepsy in mice. J Neurosurg. 120:628–638. 2014. View Article : Google Scholar :

25 

Boison D: Adenosine augmentation therapies (AATs) for epilepsy: Prospect of cell and gene therapies. Epilepsy Res. 85:131–141. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Huber A, Padrun V, Déglon N, Aebischer P, Möhler H and Boison D: Grafts of adenosine-releasing cells suppress seizures in kindling epilepsy. Proc Natl Acad Sci USA. 98:7611–7616. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Güttinger M, Padrun V, Pralong WF and Boison D: Seizure suppression and lack of adenosine A1 receptor desensitization after focal long-term delivery of adenosine by encapsulated myoblasts. Exp Neurol. 193:53–64. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Güttinger M, Fedele D, Koch P, Padrun V, Pralong WF, Brüstle O and Boison D: Suppression of kindled seizures by paracrine adenosine release from stem cell-derived brain implants. Epilepsia. 46:1162–1169. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Fedele DE, Koch P, Scheurer L, Simpson EM, Möhler H, Brüstle O and Boison D: Engineering embryonic stem cell derived glia for adenosine delivery. Neurosci Lett. 370:160–165. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang J, He Y, Chen M, Du J, Li G, Li S, Liu W and Long X: Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy. Mol Med Rep 12: 6509-6516, 2015.
APA
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S. ... Long, X. (2015). Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy. Molecular Medicine Reports, 12, 6509-6516. https://doi.org/10.3892/mmr.2015.4285
MLA
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S., Liu, W., Long, X."Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy". Molecular Medicine Reports 12.5 (2015): 6509-6516.
Chicago
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S., Liu, W., Long, X."Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy". Molecular Medicine Reports 12, no. 5 (2015): 6509-6516. https://doi.org/10.3892/mmr.2015.4285
Copy and paste a formatted citation
x
Spandidos Publications style
Huang J, He Y, Chen M, Du J, Li G, Li S, Liu W and Long X: Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy. Mol Med Rep 12: 6509-6516, 2015.
APA
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S. ... Long, X. (2015). Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy. Molecular Medicine Reports, 12, 6509-6516. https://doi.org/10.3892/mmr.2015.4285
MLA
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S., Liu, W., Long, X."Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy". Molecular Medicine Reports 12.5 (2015): 6509-6516.
Chicago
Huang, J., He, Y., Chen, M., Du, J., Li, G., Li, S., Liu, W., Long, X."Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy". Molecular Medicine Reports 12, no. 5 (2015): 6509-6516. https://doi.org/10.3892/mmr.2015.4285
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