Low temperature induces cryoinjury in mouse corneal endothelial cells by stimulating the Stk11-p53 signal pathway

Zhao,Sijie; Fei,Xinfeng; Liu,Te; Liu,Yan

doi:10.3892/mmr.2015.4301

November-2015 Volume 12 Issue 5

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November-2015 Volume 12 Issue 5

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Article Open Access

Low temperature induces cryoinjury in mouse corneal endothelial cells by stimulating the Stk11-p53 signal pathway

Authors:
- Sijie Zhao
- Xinfeng Fei
- Te Liu
- Yan Liu
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200080, P.R. China, Department of Ophthalmology, The Branch of The First People's Hospital of Shanghai, Shanghai 200081, P.R. China, Basic Research Laboratory, Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, P.R. China

Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 6612-6616
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Published online on: September 9, 2015

https://doi.org/10.3892/mmr.2015.4301
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Abstract

Cryoinjury, or injury caused by extremely low temperatures, can occur in corneal endothelial cells (CECs) and lead to visual impairment. However, the mechanism of cryoinjury in CECs is not clear. The Stk11‑p53 signaling pathway regulates the proliferation and division of cells. Activity of the Stk11‑p53 signaling pathway arrests the cell cycle at the G0/G1 phase and induces apoptosis. In this study, a mouse model of cryoinjury in CECs was used. Following injury, significant mouse CEC death and shedding were observed. In addition, the mRNA and protein levels of core factors from the Stk11‑p53 signaling pathway (Stk11, p21 and p53) were elevated and Caspase‑3 was activated following cryoinjury. In addition, chromatin immunoprecipitation revealed that Stk11 catalyzed p53 serine 15 phosphorylation, and the Stk11‑p53 complex bound to the p21 promoter and stimulated gene transcription. Thus, the results of the present study suggest that cryoinjury leads to the damage and apoptosis of mouse CECs by activation of the Stk11‑p53 signaling pathway, phosphorylation of p53 serine 15 and p21 gene transcription.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Low temperature induces cryoinjury in mouse corneal endothelial cells by stimulating the Stk11-p53 signal pathway

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