Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2015 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2015 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells

  • Authors:
    • Caroline Odewumi
    • Lekan M. Latinwo
    • Andre Sinclair
    • Veera L.D. Badisa
    • Ahkinyala Abdullah
    • Ramesh B. Badisa
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, Florida A&M University, Tallahassee, FL 32307, USA, Department of Integrated Environmental Science and Natural Science, School of Science Engineering and Math, Bethune‑Cookman University, Daytona Beach, FL 32114, USA, Department of Basic Science, College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA
    Copyright: © Odewumi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6422-6426
    |
    Published online on: September 10, 2015
       https://doi.org/10.3892/mmr.2015.4316
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Cadmium is an environmentally hazardous metal, which causes toxicity in humans. Inhalation of cigarette smoke and industrial fumes containing cadmium are sources of cadmium exposure. It is responsible for the malfunction of various organs, leading to disease particularly in the lungs, liver and kidneys. In the present study, the effect of cadmium chloride (CdCl2) on cell viability, and the expression levels of interleukin (IL)‑1α and IL‑10 cytokines at various concentrations and incubation durations were assessed in MRC‑9 human normal lung and A549 human lung cancer cells to elucidate the mechanism of cadmium toxicity. Cell viability was measured using a crystal violet dye binding assay. The expression levels of the cytokines were measured by cytokine specific enzyme‑linked immunosorbent assay kits. The viability assay results revealed higher sensitivity of the A549 lung cancer cells to CdCl2 compared with the normal MRC‑9 lung cells. In the normal MRC‑9 lung cells, higher expression levels of the cytokines were observed at the lowest CdCl2 concentration at a shorter exposure time compared with the lung cancer cells. Higher levels of the cytokines were observed in the A549 lung cancer cells at all other times and concentrations compared with the MRC‑9 cells, indicating higher levels of inflammation. The cytokine levels were reduced at higher CdCl2 concentrations and longer exposure durations, demonstrating the toxic effect of cadmium. The results indicated that CdCl2 affected the expression levels of the cytokines and led to cytotoxicity in human lung cells, and suggested that compounds which reduce inflammation may prevent cadmium toxicity.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

International agency for research on cancer (IARC): Monographs on the Evaluation of the Carcinogenic Risks to Humans, Beryllium, Cadmium, Mercury and Exposures in the Glass Manufacturing industry. IARC Scientific Publications; Lyon, France: pp. 119–238. 1993

2 

Goering PL, Waalkes MP and Klaassen CD: Toxicology of cadmium. Toxicology of metals: Biochemical Aspects. Goyer RA and Cherias M: Handb Exp Pharmacol. 15:189–214. 1995. View Article : Google Scholar

3 

Agency for Toxic Substances Disease Registry (ATSDR): Toxicological Profile for Cadmium. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service; pp. 1–512. 2012

4 

Joseph P: Mechanisms of cadmium carcinogenesis. Toxicol Appl Pharmacol. 238:272–279. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Chin TA and Templeton DM: Protective elevations of glutathione and metallothionein in cadmium-exposed mesangial cells. Toxicology. 77:145–156. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Bridges CC and Zalups RK: Molecular and ionic mimicry and the transport of toxic metals. Toxicol Appl Pharmacol. 204:274–308. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Habeebu SS, Liu J and Klaassen CD: Cadmium-induced apoptosis in mouse liver. Toxicol Appl Pharmacol. 149:203–209. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Rikans LE and Yamano T: Mechanisms of cadmium-mediated acute hepatotoxicity. J Biochem Mol Toxicol. 14:110–117. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Jin T, Lu J and Nordberg M: Toxicokinetics and biochemistry of cadmium with special emphasis on the role of metallothionein. Neurotoxicology. 19:529–535. 1998.PubMed/NCBI

10 

Waalkes MP, Anver M and Diwan BA: Carcinogenic effects of cadmium in the noble (NBL/Cr) rat: Induction of pituitary, testicular and injection site tumors and intraepithelial proliferative lesions of the dorsolateral prostate. Toxicol Sci. 52:154–161. 1999. View Article : Google Scholar

11 

Boujelben M, Ghorbel F, Vincent C, Makni-Ayadi F, Guermazi F, Croute F and El-Feki A: Lipid peroxidation and HSP72/73 expression in rat following cadmium chloride administration: Interactions of magnesium supplementation. Exp Toxicol Pathol. 57:437–443. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Ikediobi CO, Badisa VL, Ayuk-Takem LT, Latinwo LM and West J: Response of antioxidant enzymes and redox metabolites to cadmium-induced oxidative stress in CRL-1439 normal rat liver cells. Int J Mol Med. 14:87–92. 2004.PubMed/NCBI

13 

Badisa VL, Latinwo LM, Odewumi CO, Ikediobi CO, Badisa RB, Brooks-Walter A, Lambert AT and Nwoga J: Cytotoxicity and stress gene microarray analysis in cadmium-exposed CRL-1439 normal rat liver cells. Int J Mol Med. 22:213–219. 2008.PubMed/NCBI

14 

Odewumi CO, Badisa VL, Le UT, Latinwo LM, Ikediobi CO, Badisa RB and Darling-Reed SF: Protective effects of N-acetylcysteine against cadmium-induced damage in cultured rat normal liver cells. Int J Mol Med. 27:243–248. 2011. View Article : Google Scholar

15 

Odewumi CO, Buggs R, Badisa VL, Latinwo LM, Badisa RB, Ikediobi CO, Darling-Reed SF and Owens MA: Mitigative action of monoisoamy 1-2,3-dimercaptosuccinate (MiADMS) against cadmium-induced damage in cultured rat normal liver cells. Toxicol In Vitro. 25:1733–1739. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Navarro Silvera SA and Rohan TE: Trace elements and cancer risk: A review of the epidemiologic evidence. Cancer Causes Control. 18:7–27. 2007. View Article : Google Scholar

17 

Straif K, Benbrahim-Tallaa L, Baan R, Grosse Y, Secretan B, El Ghissassi F, Bouvard V, Guha N, Freeman C, Galichet L, et al WHO International Agency for Research on Cancer Monograph Working Group: A review of human Carcinogens part C: Metals, arsenic, dusts and fibres. Lancet Oncol. 10:453–454. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Kundu S, Sengupta S and Bhattacharyya A: EGFR upregulates inflammatory and proliferative responses in human lung adenocarcinoma cell line (A549), induced by lower dose of cadmium chloride. Inhal Toxicol. 23:339–348. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Rogalska J, Pilat-Marcinkiewicz B and Brzóska MM: Protective effect of zinc against cadmium hepatotoxicity depends on this bioelement intake and level of cadmium exposure: A study in a rat model. Chem Biol Interact. 193:191–203. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Shimabukuro DW, Sawa T and Gropper MA: Injury and repair in lung and airways. Crit. Care Med. 31(8 Suppl): S524–S531. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Grivennikov SI, Kuprash DV, Liu ZG and Nedospasov SA: Intracellular signals and events activated by cytokines of the tumor necrosis factor superfamily: From simple paradigms to complex mechanisms. Int Rev Cytol. 252:129–161. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Barksby HE, Lea SR, Preshaw PM and Taylor JJ: The expanding family of interleukin-1 cytokines and their role in destructive inflammatory disorders. Clin Exp Immunol. 149:217–225. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Trandem K, Jin Q, Weiss KA, James BR, Zhao J and Perlman S: Virally expressed interleukin-10 ameliorates acute encephalomyelitis and chronic demyelination in coronavirus-infected mice. J Virol. 85:6822–6831. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Odewumi CO, Fils-Aime S, Badisa VL, Latinwo LM, Ruden ML, Ikediobi C and Badisa RB: Chemoprotective effect of monoisoamyl 2, 3-dimercaptosuccinate (MiADMS) on cytokines expression in cadmium chloride treated human lung cells. Environ Toxicol. 30:704–711. 2015. View Article : Google Scholar

25 

Badisa RB, Tzakou O, Couladis M and Pilarinou E: Cytotoxic activities of some Greek Labiatae herbs. Phytother Res. 17:472–476. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Ipsen J and Feigl P: Bancroft's Introduction to Biostatistics. Second Edition. Harper and Row; New York: pp. 1641970

27 

Krocova Z, Macela A, Kroca M and Hernychova L: The immu-nomodulatory effect(s) of lead and cadmium on the cells of immune system in vitro. Toxicol In Vitro. 14:33–40. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Janssen AM, van Duijn W, Kubben FJ, Griffioen G, Lamers CB, van Krieken JH, van de Velde CJ and Verspaget HW: Prognostic significance of metallothionein in human gastrointestinal cancer. Clin Cancer Res. 8:1889–1896. 2002.PubMed/NCBI

29 

Balharry D, Sexton K and BéruBé KA: An in vitro approach to assess the toxicity of inhaled tobacco smoke components: Nicotine, cadmium, formaldehyde and urethane. Toxicology. 244:66–76. 2008. View Article : Google Scholar

30 

Bergman M, Djaldetti M, Salman H and Bessler H: Inflammation and colorectal cancer: Does aspirin affect the interaction between cancer and immune cells? Inflammation. 34:22–28. 2011. View Article : Google Scholar

31 

Romoser AA, Chen PL, Berg JM, Seabury C, Ivanov I, Criscitiello MF and Sayes CM: Quantum dots trigger immunomodulation of the NFκB pathway in human skin cells. Mol Immunol. 48:1349–1359. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Kayama F, Yoshida T, Elwell MR and Luster MI: Role of tumor necrosis factor-alpha in cadmium-induced hepatotoxicity. Toxicol Appl Pharmacol. 131:224–234. 1995. View Article : Google Scholar : PubMed/NCBI

33 

Kawano Y, Furukawa Y, Kawano Y, Abe W, Hirakawa T and Narahara H: Cadmium chloride induces the expression of metallothionein mRNA by endometrial stromal cells and amnion-derived (WISH) cells. Gynecol Obstet Invest. 71:240–244. 2011. View Article : Google Scholar

34 

Katsumata N, Eguchi K, Fukuda M, Yamamoto N, Ohe Y, Oshita F, Tamura T, Shinkai T and Saijo N: Serum levels of cytokines in patients with untreated primary lung cancer. Clin Cancer Res. 2:553–559. 1996.PubMed/NCBI

35 

Bermúdez-Morales VH, Peralta-Zaragoza O, Alcocer-González JM, Moreno J and Madrid-Marina V: IL-10 expression is regulated by HPV E2 protein in cervical cancer cells. Mol Med Report. 4:369–375. 2011.

36 

Thévenod F and Lee WK: Cadmium and cellular signaling cascades: Interactions between cell death and survival pathways. Arch Toxicol. 87:1743–1786. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Odewumi C, Latinwo LM, Sinclair A, Badisa VL, Abdullah A and Badisa RB: Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells. Mol Med Rep 12: 6422-6426, 2015.
APA
Odewumi, C., Latinwo, L.M., Sinclair, A., Badisa, V.L., Abdullah, A., & Badisa, R.B. (2015). Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells. Molecular Medicine Reports, 12, 6422-6426. https://doi.org/10.3892/mmr.2015.4316
MLA
Odewumi, C., Latinwo, L. M., Sinclair, A., Badisa, V. L., Abdullah, A., Badisa, R. B."Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells". Molecular Medicine Reports 12.5 (2015): 6422-6426.
Chicago
Odewumi, C., Latinwo, L. M., Sinclair, A., Badisa, V. L., Abdullah, A., Badisa, R. B."Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells". Molecular Medicine Reports 12, no. 5 (2015): 6422-6426. https://doi.org/10.3892/mmr.2015.4316
Copy and paste a formatted citation
x
Spandidos Publications style
Odewumi C, Latinwo LM, Sinclair A, Badisa VL, Abdullah A and Badisa RB: Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells. Mol Med Rep 12: 6422-6426, 2015.
APA
Odewumi, C., Latinwo, L.M., Sinclair, A., Badisa, V.L., Abdullah, A., & Badisa, R.B. (2015). Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells. Molecular Medicine Reports, 12, 6422-6426. https://doi.org/10.3892/mmr.2015.4316
MLA
Odewumi, C., Latinwo, L. M., Sinclair, A., Badisa, V. L., Abdullah, A., Badisa, R. B."Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells". Molecular Medicine Reports 12.5 (2015): 6422-6426.
Chicago
Odewumi, C., Latinwo, L. M., Sinclair, A., Badisa, V. L., Abdullah, A., Badisa, R. B."Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells". Molecular Medicine Reports 12, no. 5 (2015): 6422-6426. https://doi.org/10.3892/mmr.2015.4316
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team